Characterization and seasonal changes in LHβ and FSHβ mRNA of Rhinella arenarum (Amphibia, Anura)

In anurans, two types of gonadotropins were described in several species of Ranidae and Pipidae families but only in one of the Bufonidae family. Rhinella arenarum is a bufonid that have the lowest concentration of plasma androgens during the breeding. The objective of this paper was to characterize...

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Autores principales:	Volonteri, María Clara, Regueira, Eleonora, Scaia, María Florencia, Ceballos, Nora Raquel
Publicado:	2013
Materias:	Androgens Gonadotropins Pituitary Seasonal variations Toad amino acid complementary DNA cysteine follitropin beta subunit luteinizing hormone luteinizing hormone beta messenger RNA unclassified drug 3' untranslated region 5' untranslated region Amphibia Anura article bird controlled study gene sequence human male mammal mouse nonhuman nucleotide sequence open reading frame priority journal protein glycosylation quantitative analysis reverse transcription polymerase chain reaction Rhinella arenarum seasonal variation sequence alignment signal transduction teleost tetrapod
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00166480_v187_n_p95_Volonteri http://hdl.handle.net/20.500.12110/paper_00166480_v187_n_p95_Volonteri
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00166480_v187_n_p95_Volonteri
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spelling	paper:paper_00166480_v187_n_p95_Volonteri2023-06-08T14:38:20Z Characterization and seasonal changes in LHβ and FSHβ mRNA of Rhinella arenarum (Amphibia, Anura) Volonteri, María Clara Regueira, Eleonora Scaia, María Florencia Ceballos, Nora Raquel Androgens Gonadotropins Pituitary Seasonal variations Toad amino acid complementary DNA cysteine follitropin beta subunit luteinizing hormone luteinizing hormone beta messenger RNA unclassified drug 3' untranslated region 5' untranslated region Amphibia Anura article bird controlled study gene sequence human male mammal mouse nonhuman nucleotide sequence open reading frame priority journal protein glycosylation quantitative analysis reverse transcription polymerase chain reaction Rhinella arenarum seasonal variation sequence alignment signal transduction teleost tetrapod In anurans, two types of gonadotropins were described in several species of Ranidae and Pipidae families but only in one of the Bufonidae family. Rhinella arenarum is a bufonid that have the lowest concentration of plasma androgens during the breeding. The objective of this paper was to characterize the cDNA sequence of β subunit of LH and FSH from toad pituitary and study seasonal variation in gonadotropins mRNA using quantitative real-time RT-PCR. The LHβ cDNA is a 636. bp sequence containing an open reading frame (ORF), 45. bp of 5'-untranslated region (UTR) and 174. bp of 3'-UTR. The ORF encodes for a signal peptide of 26 amino acids and a mature protein of 113 amino acids with one N-glycosylation site at the 34th position. The FSHβ cDNA sequence is a 535. bp fragment containing an ORF, 8. bp of 5'-UTR and 152. bp of 3'-UTR. The ORF encodes for a signal peptide of 20 amino acids and a mature protein of 104 amino acids with two N-glycosylation sites at 25th and 42nd positions. Multiple alignments of aminoacid deduced sequences of LHβ and FSHβ (teleosts, amphibians, birds, mammals) showed that all the tetrapods studied conserve 12 cysteins and one (LH) or two (FSH) N-Glycosylation sites. LHβ is closer to teleosts than to mammals and birds while FSHβ is closer to mammals. The analysis of seasonal changes in LHβ and FSHβ mRNA indicates that transcript levels have seasonal variations and that the profile of androgens is opposite to that of the gonadotropins mRNA. © 2013 Elsevier Inc. Fil:Volonteri, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Regueira, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Scaia, M.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ceballos, N.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2013 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00166480_v187_n_p95_Volonteri http://hdl.handle.net/20.500.12110/paper_00166480_v187_n_p95_Volonteri
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Androgens Gonadotropins Pituitary Seasonal variations Toad amino acid complementary DNA cysteine follitropin beta subunit luteinizing hormone luteinizing hormone beta messenger RNA unclassified drug 3' untranslated region 5' untranslated region Amphibia Anura article bird controlled study gene sequence human male mammal mouse nonhuman nucleotide sequence open reading frame priority journal protein glycosylation quantitative analysis reverse transcription polymerase chain reaction Rhinella arenarum seasonal variation sequence alignment signal transduction teleost tetrapod
spellingShingle	Androgens Gonadotropins Pituitary Seasonal variations Toad amino acid complementary DNA cysteine follitropin beta subunit luteinizing hormone luteinizing hormone beta messenger RNA unclassified drug 3' untranslated region 5' untranslated region Amphibia Anura article bird controlled study gene sequence human male mammal mouse nonhuman nucleotide sequence open reading frame priority journal protein glycosylation quantitative analysis reverse transcription polymerase chain reaction Rhinella arenarum seasonal variation sequence alignment signal transduction teleost tetrapod Volonteri, María Clara Regueira, Eleonora Scaia, María Florencia Ceballos, Nora Raquel Characterization and seasonal changes in LHβ and FSHβ mRNA of Rhinella arenarum (Amphibia, Anura)
topic_facet	Androgens Gonadotropins Pituitary Seasonal variations Toad amino acid complementary DNA cysteine follitropin beta subunit luteinizing hormone luteinizing hormone beta messenger RNA unclassified drug 3' untranslated region 5' untranslated region Amphibia Anura article bird controlled study gene sequence human male mammal mouse nonhuman nucleotide sequence open reading frame priority journal protein glycosylation quantitative analysis reverse transcription polymerase chain reaction Rhinella arenarum seasonal variation sequence alignment signal transduction teleost tetrapod
description	In anurans, two types of gonadotropins were described in several species of Ranidae and Pipidae families but only in one of the Bufonidae family. Rhinella arenarum is a bufonid that have the lowest concentration of plasma androgens during the breeding. The objective of this paper was to characterize the cDNA sequence of β subunit of LH and FSH from toad pituitary and study seasonal variation in gonadotropins mRNA using quantitative real-time RT-PCR. The LHβ cDNA is a 636. bp sequence containing an open reading frame (ORF), 45. bp of 5'-untranslated region (UTR) and 174. bp of 3'-UTR. The ORF encodes for a signal peptide of 26 amino acids and a mature protein of 113 amino acids with one N-glycosylation site at the 34th position. The FSHβ cDNA sequence is a 535. bp fragment containing an ORF, 8. bp of 5'-UTR and 152. bp of 3'-UTR. The ORF encodes for a signal peptide of 20 amino acids and a mature protein of 104 amino acids with two N-glycosylation sites at 25th and 42nd positions. Multiple alignments of aminoacid deduced sequences of LHβ and FSHβ (teleosts, amphibians, birds, mammals) showed that all the tetrapods studied conserve 12 cysteins and one (LH) or two (FSH) N-Glycosylation sites. LHβ is closer to teleosts than to mammals and birds while FSHβ is closer to mammals. The analysis of seasonal changes in LHβ and FSHβ mRNA indicates that transcript levels have seasonal variations and that the profile of androgens is opposite to that of the gonadotropins mRNA. © 2013 Elsevier Inc.
author	Volonteri, María Clara Regueira, Eleonora Scaia, María Florencia Ceballos, Nora Raquel
author_facet	Volonteri, María Clara Regueira, Eleonora Scaia, María Florencia Ceballos, Nora Raquel
author_sort	Volonteri, María Clara
title	Characterization and seasonal changes in LHβ and FSHβ mRNA of Rhinella arenarum (Amphibia, Anura)
title_short	Characterization and seasonal changes in LHβ and FSHβ mRNA of Rhinella arenarum (Amphibia, Anura)
title_full	Characterization and seasonal changes in LHβ and FSHβ mRNA of Rhinella arenarum (Amphibia, Anura)
title_fullStr	Characterization and seasonal changes in LHβ and FSHβ mRNA of Rhinella arenarum (Amphibia, Anura)
title_full_unstemmed	Characterization and seasonal changes in LHβ and FSHβ mRNA of Rhinella arenarum (Amphibia, Anura)
title_sort	characterization and seasonal changes in lhβ and fshβ mrna of rhinella arenarum (amphibia, anura)
publishDate	2013
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00166480_v187_n_p95_Volonteri http://hdl.handle.net/20.500.12110/paper_00166480_v187_n_p95_Volonteri
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_version_	1768544439682203648

Characterization and seasonal changes in LHβ and FSHβ mRNA of Rhinella arenarum (Amphibia, Anura)

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