Arginine-vasotocin expression and participation in reproduction and social behavior in males of the cichlid fish cichlasoma dimerus
In non-mammalian vertebrates, the nonapeptide arginine-vasotocin (AVT) is involved in the regulation of social behavior related to reproduction and aggression. The cichlid fish Cichlasoma dimerus is a monogamous species with complex social hierarchies. Males are found in one of two basic alternative...
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2012
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00166480_v179_n2_p221_Ramallo http://hdl.handle.net/20.500.12110/paper_00166480_v179_n2_p221_Ramallo |
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paper:paper_00166480_v179_n2_p221_Ramallo2023-06-08T14:38:20Z Arginine-vasotocin expression and participation in reproduction and social behavior in males of the cichlid fish cichlasoma dimerus Ramallo, Martín R. Cánepa, Maximiliano Martín Pandolfi, Matías Cichlids Gonadal androgens Neuroendocrinology Pituitary culture Reproduction androgen argiprestocin gonadotropin luteinizing hormone androgen argiprestocin androgen release androgen synthesis animal tissue article brain nerve cell Cichlasoma dimerus cichlid concentration response controlled study gonadotropin release hypothalamus hypophysis gonad system in vitro study Leydig cell male morphometrics nonhuman priority journal protein expression reproduction reverse transcription polymerase chain reaction social behavior social status testis vasotocinergic neuron adult animal animal cell animal experiment female gene expression hormone action hormone response hormone synthesis immunohistochemistry nerve cell neuroendocrine system nucleotide sequence vasotocinergic nerve cell vasotocinergic system animal animal behavior biosynthesis brain drug effect hypothalamus hypophysis system metabolism physiology sexual behavior social behavior social dominance territoriality Androgens Animals Behavior, Animal Brain Cichlids Hypothalamo-Hypophyseal System Male Neurons Sexual Behavior, Animal Social Behavior Social Dominance Territoriality Testis Vasotocin In non-mammalian vertebrates, the nonapeptide arginine-vasotocin (AVT) is involved in the regulation of social behavior related to reproduction and aggression. The cichlid fish Cichlasoma dimerus is a monogamous species with complex social hierarchies. Males are found in one of two basic alternative phenotypes: Non-territorial and territorial males. In this work we characterize the vasotocinergic system in males of C. dimerus in relation to social status with particular emphasis on the various putative sites of action of AVT across the hypothalamic-pituitary-gonad (HPG) axis, and its effects on reproductive and social behavior. The location and distribution of vasotocinergic neurons in the brain was studied, highlighting a morphometric analysis of AVT producing neurons in males of different social status. The effect of AVT on pituitary gonadotropin secretion was analyzed by single pituitary culture while expression of AVT in peripheral organs was studied by RT-PCR using specific primers. Finally, the role of AVT on testicular androgen release was assessed by in vitro incubation of testis. Results showed a positive effect of AVT on gonadotropin secretion, where β-LH showcased a triphasic response under increasing AVT concentration, while β-FSH's response was dose-dependent and directly proportional. AVT showed a positive and concentration-dependent effect over testicular androgens synthesis and secretion in vitro. Vasotocin expression was observed in testicular somatic tissue located in the interstitial compartment. Thus, the AVT system in C. dimerus appears to be of high complexity, with multiple sites of action in the hypothalamus-pituitary-gonadal axis. © 2012 Elsevier Inc. Fil:Ramallo, M.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cánepa, M.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pandolfi, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2012 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00166480_v179_n2_p221_Ramallo http://hdl.handle.net/20.500.12110/paper_00166480_v179_n2_p221_Ramallo |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Cichlids Gonadal androgens Neuroendocrinology Pituitary culture Reproduction androgen argiprestocin gonadotropin luteinizing hormone androgen argiprestocin androgen release androgen synthesis animal tissue article brain nerve cell Cichlasoma dimerus cichlid concentration response controlled study gonadotropin release hypothalamus hypophysis gonad system in vitro study Leydig cell male morphometrics nonhuman priority journal protein expression reproduction reverse transcription polymerase chain reaction social behavior social status testis vasotocinergic neuron adult animal animal cell animal experiment female gene expression hormone action hormone response hormone synthesis immunohistochemistry nerve cell neuroendocrine system nucleotide sequence vasotocinergic nerve cell vasotocinergic system animal animal behavior biosynthesis brain drug effect hypothalamus hypophysis system metabolism physiology sexual behavior social behavior social dominance territoriality Androgens Animals Behavior, Animal Brain Cichlids Hypothalamo-Hypophyseal System Male Neurons Sexual Behavior, Animal Social Behavior Social Dominance Territoriality Testis Vasotocin |
| spellingShingle |
Cichlids Gonadal androgens Neuroendocrinology Pituitary culture Reproduction androgen argiprestocin gonadotropin luteinizing hormone androgen argiprestocin androgen release androgen synthesis animal tissue article brain nerve cell Cichlasoma dimerus cichlid concentration response controlled study gonadotropin release hypothalamus hypophysis gonad system in vitro study Leydig cell male morphometrics nonhuman priority journal protein expression reproduction reverse transcription polymerase chain reaction social behavior social status testis vasotocinergic neuron adult animal animal cell animal experiment female gene expression hormone action hormone response hormone synthesis immunohistochemistry nerve cell neuroendocrine system nucleotide sequence vasotocinergic nerve cell vasotocinergic system animal animal behavior biosynthesis brain drug effect hypothalamus hypophysis system metabolism physiology sexual behavior social behavior social dominance territoriality Androgens Animals Behavior, Animal Brain Cichlids Hypothalamo-Hypophyseal System Male Neurons Sexual Behavior, Animal Social Behavior Social Dominance Territoriality Testis Vasotocin Ramallo, Martín R. Cánepa, Maximiliano Martín Pandolfi, Matías Arginine-vasotocin expression and participation in reproduction and social behavior in males of the cichlid fish cichlasoma dimerus |
| topic_facet |
Cichlids Gonadal androgens Neuroendocrinology Pituitary culture Reproduction androgen argiprestocin gonadotropin luteinizing hormone androgen argiprestocin androgen release androgen synthesis animal tissue article brain nerve cell Cichlasoma dimerus cichlid concentration response controlled study gonadotropin release hypothalamus hypophysis gonad system in vitro study Leydig cell male morphometrics nonhuman priority journal protein expression reproduction reverse transcription polymerase chain reaction social behavior social status testis vasotocinergic neuron adult animal animal cell animal experiment female gene expression hormone action hormone response hormone synthesis immunohistochemistry nerve cell neuroendocrine system nucleotide sequence vasotocinergic nerve cell vasotocinergic system animal animal behavior biosynthesis brain drug effect hypothalamus hypophysis system metabolism physiology sexual behavior social behavior social dominance territoriality Androgens Animals Behavior, Animal Brain Cichlids Hypothalamo-Hypophyseal System Male Neurons Sexual Behavior, Animal Social Behavior Social Dominance Territoriality Testis Vasotocin |
| description |
In non-mammalian vertebrates, the nonapeptide arginine-vasotocin (AVT) is involved in the regulation of social behavior related to reproduction and aggression. The cichlid fish Cichlasoma dimerus is a monogamous species with complex social hierarchies. Males are found in one of two basic alternative phenotypes: Non-territorial and territorial males. In this work we characterize the vasotocinergic system in males of C. dimerus in relation to social status with particular emphasis on the various putative sites of action of AVT across the hypothalamic-pituitary-gonad (HPG) axis, and its effects on reproductive and social behavior. The location and distribution of vasotocinergic neurons in the brain was studied, highlighting a morphometric analysis of AVT producing neurons in males of different social status. The effect of AVT on pituitary gonadotropin secretion was analyzed by single pituitary culture while expression of AVT in peripheral organs was studied by RT-PCR using specific primers. Finally, the role of AVT on testicular androgen release was assessed by in vitro incubation of testis. Results showed a positive effect of AVT on gonadotropin secretion, where β-LH showcased a triphasic response under increasing AVT concentration, while β-FSH's response was dose-dependent and directly proportional. AVT showed a positive and concentration-dependent effect over testicular androgens synthesis and secretion in vitro. Vasotocin expression was observed in testicular somatic tissue located in the interstitial compartment. Thus, the AVT system in C. dimerus appears to be of high complexity, with multiple sites of action in the hypothalamus-pituitary-gonadal axis. © 2012 Elsevier Inc. |
| author |
Ramallo, Martín R. Cánepa, Maximiliano Martín Pandolfi, Matías |
| author_facet |
Ramallo, Martín R. Cánepa, Maximiliano Martín Pandolfi, Matías |
| author_sort |
Ramallo, Martín R. |
| title |
Arginine-vasotocin expression and participation in reproduction and social behavior in males of the cichlid fish cichlasoma dimerus |
| title_short |
Arginine-vasotocin expression and participation in reproduction and social behavior in males of the cichlid fish cichlasoma dimerus |
| title_full |
Arginine-vasotocin expression and participation in reproduction and social behavior in males of the cichlid fish cichlasoma dimerus |
| title_fullStr |
Arginine-vasotocin expression and participation in reproduction and social behavior in males of the cichlid fish cichlasoma dimerus |
| title_full_unstemmed |
Arginine-vasotocin expression and participation in reproduction and social behavior in males of the cichlid fish cichlasoma dimerus |
| title_sort |
arginine-vasotocin expression and participation in reproduction and social behavior in males of the cichlid fish cichlasoma dimerus |
| publishDate |
2012 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00166480_v179_n2_p221_Ramallo http://hdl.handle.net/20.500.12110/paper_00166480_v179_n2_p221_Ramallo |
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