Cloning, phylogenetic analysis and expression of somatolactin and its receptor in Cichlasoma dimerus: Their role in long-term background color acclimation

Somatolactin (SL) and SL receptor (SLR) belong to the growth hormone and cytokine type I receptor superfamilies, respectively. However, further research is required to define the duplications and functions of SL and its receptors in basal vertebrates including environmental background color adaptati...

Descripción completa

Detalles Bibliográficos
Autores principales: Cánepa, Maximiliano Martín, Fossati, Mariana, Vissio, Paula Gabriela
Publicado: 2012
Materias:
Background color exposure
Chromatophore
Cichlid
Melanophore
Pituitary
Somatolactin
Somatolactin receptor
Teleost
growth hormone receptor
messenger RNA
somatolectin
somatolectin receptor
unclassified drug
acclimatization
animal cell
animal experiment
article
Cichlasoma dimerus
cichlid
color
controlled study
epidermis cell
female
gene sequence
male
melanophore
molecular cloning
nonhuman
nucleotide sequence
Perciformes
phylogeny
priority journal
protein expression
salmonid
skin cell
species differentiation
unindexed sequence
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00166480_v176_n1_p52_Canepa
http://hdl.handle.net/20.500.12110/paper_00166480_v176_n1_p52_Canepa
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_00166480_v176_n1_p52_Canepa
record_format dspace
spelling paper:paper_00166480_v176_n1_p52_Canepa2023-06-08T14:38:19Z Cloning, phylogenetic analysis and expression of somatolactin and its receptor in Cichlasoma dimerus: Their role in long-term background color acclimation Cánepa, Maximiliano Martín Fossati, Mariana Vissio, Paula Gabriela Background color exposure Chromatophore Cichlid Melanophore Pituitary Somatolactin Somatolactin receptor Teleost growth hormone receptor messenger RNA somatolectin somatolectin receptor unclassified drug acclimatization animal cell animal experiment article Cichlasoma dimerus cichlid color controlled study epidermis cell female gene sequence male melanophore molecular cloning nonhuman nucleotide sequence Perciformes phylogeny priority journal protein expression salmonid skin cell species differentiation unindexed sequence Somatolactin (SL) and SL receptor (SLR) belong to the growth hormone and cytokine type I receptor superfamilies, respectively. However, further research is required to define the duplications and functions of SL and its receptors in basal vertebrates including environmental background color adaptation in fish. In the present study, we cloned and sequenced SL and its putative receptor (SLR), classified and compared the sequences phylogenetically, and determined SL and SLR mRNA expression levels during long-term background color exposure in Cichlasoma dimerus, a freshwater South American cichlid. Our results show that C. dimerus SL and SLR share high sequence similarity with homologous from other perciform fish. Phylogenetic analysis indicates that C. dimerus SL belongs to the SLα clade sub-group. C. dimerus SLR is clearly a member of the GHR1 receptor subgroup, which includes the experimentally validated SLR from salmonids. Higher transcript levels of SLα in the pituitary and SLR in the epidermis and dermis cells of fish scales were observed in fish following long-term black background color exposure compared to those exposed to a white background. A higher number of melanophores was also observed in fish exposed for 10. days to a black background compared to those exposed to a white background. These changes were concomitant to differences in SL or SLR transcript levels found in fish exposed to these two different background colors. Our results suggest, for the first time, that SLR is expressed in fish scales, and that there is an increase in SL in the pituitary and the putative SLR in likely target cells, i.e., melanophores, in long-term black background exposure in C. dimerus. © 2011 Elsevier Inc. Fil:Cánepa, M.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Fossati, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Vissio, P.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2012 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00166480_v176_n1_p52_Canepa http://hdl.handle.net/20.500.12110/paper_00166480_v176_n1_p52_Canepa
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Background color exposure
Chromatophore
Cichlid
Melanophore
Pituitary
Somatolactin
Somatolactin receptor
Teleost
growth hormone receptor
messenger RNA
somatolectin
somatolectin receptor
unclassified drug
acclimatization
animal cell
animal experiment
article
Cichlasoma dimerus
cichlid
color
controlled study
epidermis cell
female
gene sequence
male
melanophore
molecular cloning
nonhuman
nucleotide sequence
Perciformes
phylogeny
priority journal
protein expression
salmonid
skin cell
species differentiation
unindexed sequence
spellingShingle Background color exposure
Chromatophore
Cichlid
Melanophore
Pituitary
Somatolactin
Somatolactin receptor
Teleost
growth hormone receptor
messenger RNA
somatolectin
somatolectin receptor
unclassified drug
acclimatization
animal cell
animal experiment
article
Cichlasoma dimerus
cichlid
color
controlled study
epidermis cell
female
gene sequence
male
melanophore
molecular cloning
nonhuman
nucleotide sequence
Perciformes
phylogeny
priority journal
protein expression
salmonid
skin cell
species differentiation
unindexed sequence
Cánepa, Maximiliano Martín
Fossati, Mariana
Vissio, Paula Gabriela
Cloning, phylogenetic analysis and expression of somatolactin and its receptor in Cichlasoma dimerus: Their role in long-term background color acclimation
topic_facet Background color exposure
Chromatophore
Cichlid
Melanophore
Pituitary
Somatolactin
Somatolactin receptor
Teleost
growth hormone receptor
messenger RNA
somatolectin
somatolectin receptor
unclassified drug
acclimatization
animal cell
animal experiment
article
Cichlasoma dimerus
cichlid
color
controlled study
epidermis cell
female
gene sequence
male
melanophore
molecular cloning
nonhuman
nucleotide sequence
Perciformes
phylogeny
priority journal
protein expression
salmonid
skin cell
species differentiation
unindexed sequence
description Somatolactin (SL) and SL receptor (SLR) belong to the growth hormone and cytokine type I receptor superfamilies, respectively. However, further research is required to define the duplications and functions of SL and its receptors in basal vertebrates including environmental background color adaptation in fish. In the present study, we cloned and sequenced SL and its putative receptor (SLR), classified and compared the sequences phylogenetically, and determined SL and SLR mRNA expression levels during long-term background color exposure in Cichlasoma dimerus, a freshwater South American cichlid. Our results show that C. dimerus SL and SLR share high sequence similarity with homologous from other perciform fish. Phylogenetic analysis indicates that C. dimerus SL belongs to the SLα clade sub-group. C. dimerus SLR is clearly a member of the GHR1 receptor subgroup, which includes the experimentally validated SLR from salmonids. Higher transcript levels of SLα in the pituitary and SLR in the epidermis and dermis cells of fish scales were observed in fish following long-term black background color exposure compared to those exposed to a white background. A higher number of melanophores was also observed in fish exposed for 10. days to a black background compared to those exposed to a white background. These changes were concomitant to differences in SL or SLR transcript levels found in fish exposed to these two different background colors. Our results suggest, for the first time, that SLR is expressed in fish scales, and that there is an increase in SL in the pituitary and the putative SLR in likely target cells, i.e., melanophores, in long-term black background exposure in C. dimerus. © 2011 Elsevier Inc.
author Cánepa, Maximiliano Martín
Fossati, Mariana
Vissio, Paula Gabriela
author_facet Cánepa, Maximiliano Martín
Fossati, Mariana
Vissio, Paula Gabriela
author_sort Cánepa, Maximiliano Martín
title Cloning, phylogenetic analysis and expression of somatolactin and its receptor in Cichlasoma dimerus: Their role in long-term background color acclimation
title_short Cloning, phylogenetic analysis and expression of somatolactin and its receptor in Cichlasoma dimerus: Their role in long-term background color acclimation
title_full Cloning, phylogenetic analysis and expression of somatolactin and its receptor in Cichlasoma dimerus: Their role in long-term background color acclimation
title_fullStr Cloning, phylogenetic analysis and expression of somatolactin and its receptor in Cichlasoma dimerus: Their role in long-term background color acclimation
title_full_unstemmed Cloning, phylogenetic analysis and expression of somatolactin and its receptor in Cichlasoma dimerus: Their role in long-term background color acclimation
title_sort cloning, phylogenetic analysis and expression of somatolactin and its receptor in cichlasoma dimerus: their role in long-term background color acclimation
publishDate 2012
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00166480_v176_n1_p52_Canepa
http://hdl.handle.net/20.500.12110/paper_00166480_v176_n1_p52_Canepa
work_keys_str_mv AT canepamaximilianomartin cloningphylogeneticanalysisandexpressionofsomatolactinanditsreceptorincichlasomadimerustheirroleinlongtermbackgroundcoloracclimation
AT fossatimariana cloningphylogeneticanalysisandexpressionofsomatolactinanditsreceptorincichlasomadimerustheirroleinlongtermbackgroundcoloracclimation
AT vissiopaulagabriela cloningphylogeneticanalysisandexpressionofsomatolactinanditsreceptorincichlasomadimerustheirroleinlongtermbackgroundcoloracclimation
_version_ 1768544023397531648

Ejemplares similares