Gonadotropin-releasing hormone (GnRH) variants in a lizard brain: Is mammalian GnRH being expressed?

In reptiles as in other vertebrates, multiple forms of gonadotropin-releasing hormone (GnRH) within a single brain have been identified. In this group the following GnRH molecular variants have been characterized either by direct or indirect methods: chicken GnRH I (cGnRH-I), chicken GnRH II (cGnRH-...

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Detalles Bibliográficos
Publicado: 2000
Materias:
Brain
Gonadotropin-releasing hormone
Reptiles
RIA
RP-HPLC
antiserum
brain extract
gonadorelin
animal experiment
animal tissue
article
brain
female
high performance liquid chromatography
hormone determination
hormone release
immunoreactivity
lizard
male
nonhuman
priority journal
protein expression
radioimmunoassay
reptile
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00166480_v119_n2_p121_Montaner
http://hdl.handle.net/20.500.12110/paper_00166480_v119_n2_p121_Montaner
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_00166480_v119_n2_p121_Montaner
record_format dspace
spelling paper:paper_00166480_v119_n2_p121_Montaner2023-06-08T14:38:16Z Gonadotropin-releasing hormone (GnRH) variants in a lizard brain: Is mammalian GnRH being expressed? Brain Gonadotropin-releasing hormone Reptiles RIA RP-HPLC antiserum brain extract gonadorelin animal experiment animal tissue article brain female high performance liquid chromatography hormone determination hormone release immunoreactivity lizard male nonhuman priority journal protein expression radioimmunoassay reptile In reptiles as in other vertebrates, multiple forms of gonadotropin-releasing hormone (GnRH) within a single brain have been identified. In this group the following GnRH molecular variants have been characterized either by direct or indirect methods: chicken GnRH I (cGnRH-I), chicken GnRH II (cGnRH-II), salmon GnRH (sGnRH) and several unidentified GnRH-like forms. In the present study GnRH variants were investigated in brain extracts of the lizard Tupinambis teguixin (= T. merinae) by combining high-performance liquid chromatography (RP-HPLC) followed by radioimmunoassays (RIA). Two peaks showing GnRH immunoreactivity with the elution position of synthetic mammalian GnRH (mGnRH) and cGnRH-II were detected. Both peaks were further analyzed with different radioimmunoassay systems specific for mGnRH, cGnRH-I, and cGnRH-II. Pooled fractions corresponding to the first eluting peak showed no cross-reactivity when analyzed with a cGnRH-I specific assay and logit-log displacement curves were not significantly different from those of synthetic mGnRH with homologous RIA systems. The second peak showed immunological characteristics of cGnRH-II when analyzed with a specific antiserum. The first ir-GnRH peak was selected for further RP-HPLC purification showing similar chromatographic behavior as mGnRH synthetic standard. We demonstrated the absence of cGnRH-I in this lizard using well-characterized antisera. (C) 2000 Academic Press. 2000 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00166480_v119_n2_p121_Montaner http://hdl.handle.net/20.500.12110/paper_00166480_v119_n2_p121_Montaner
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Brain
Gonadotropin-releasing hormone
Reptiles
RIA
RP-HPLC
antiserum
brain extract
gonadorelin
animal experiment
animal tissue
article
brain
female
high performance liquid chromatography
hormone determination
hormone release
immunoreactivity
lizard
male
nonhuman
priority journal
protein expression
radioimmunoassay
reptile
spellingShingle Brain
Gonadotropin-releasing hormone
Reptiles
RIA
RP-HPLC
antiserum
brain extract
gonadorelin
animal experiment
animal tissue
article
brain
female
high performance liquid chromatography
hormone determination
hormone release
immunoreactivity
lizard
male
nonhuman
priority journal
protein expression
radioimmunoassay
reptile
Gonadotropin-releasing hormone (GnRH) variants in a lizard brain: Is mammalian GnRH being expressed?
topic_facet Brain
Gonadotropin-releasing hormone
Reptiles
RIA
RP-HPLC
antiserum
brain extract
gonadorelin
animal experiment
animal tissue
article
brain
female
high performance liquid chromatography
hormone determination
hormone release
immunoreactivity
lizard
male
nonhuman
priority journal
protein expression
radioimmunoassay
reptile
description In reptiles as in other vertebrates, multiple forms of gonadotropin-releasing hormone (GnRH) within a single brain have been identified. In this group the following GnRH molecular variants have been characterized either by direct or indirect methods: chicken GnRH I (cGnRH-I), chicken GnRH II (cGnRH-II), salmon GnRH (sGnRH) and several unidentified GnRH-like forms. In the present study GnRH variants were investigated in brain extracts of the lizard Tupinambis teguixin (= T. merinae) by combining high-performance liquid chromatography (RP-HPLC) followed by radioimmunoassays (RIA). Two peaks showing GnRH immunoreactivity with the elution position of synthetic mammalian GnRH (mGnRH) and cGnRH-II were detected. Both peaks were further analyzed with different radioimmunoassay systems specific for mGnRH, cGnRH-I, and cGnRH-II. Pooled fractions corresponding to the first eluting peak showed no cross-reactivity when analyzed with a cGnRH-I specific assay and logit-log displacement curves were not significantly different from those of synthetic mGnRH with homologous RIA systems. The second peak showed immunological characteristics of cGnRH-II when analyzed with a specific antiserum. The first ir-GnRH peak was selected for further RP-HPLC purification showing similar chromatographic behavior as mGnRH synthetic standard. We demonstrated the absence of cGnRH-I in this lizard using well-characterized antisera. (C) 2000 Academic Press.
title Gonadotropin-releasing hormone (GnRH) variants in a lizard brain: Is mammalian GnRH being expressed?
title_short Gonadotropin-releasing hormone (GnRH) variants in a lizard brain: Is mammalian GnRH being expressed?
title_full Gonadotropin-releasing hormone (GnRH) variants in a lizard brain: Is mammalian GnRH being expressed?
title_fullStr Gonadotropin-releasing hormone (GnRH) variants in a lizard brain: Is mammalian GnRH being expressed?
title_full_unstemmed Gonadotropin-releasing hormone (GnRH) variants in a lizard brain: Is mammalian GnRH being expressed?
title_sort gonadotropin-releasing hormone (gnrh) variants in a lizard brain: is mammalian gnrh being expressed?
publishDate 2000
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00166480_v119_n2_p121_Montaner
http://hdl.handle.net/20.500.12110/paper_00166480_v119_n2_p121_Montaner
_version_ 1768541687686103040

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