Nitric oxide induces gelatinase A (matrix metalloproteinase 2) during rat embryo implantation

Objective: To evaluate a reciprocal signaling interaction initiated by embryo-derived nitric oxide (NO) to facilitate implantation by increased production of gelatinase A (matrix metalloproteinase 2, MMP2) in uterine stroma. Design: Experimental animal studies. Setting: Reproductive-physiology resea...

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Autores principales:	Novaro, Virginia, Pustovrh, Maria Carolina, Colman Lerner, Alejandro Ariel, Lo Nostro, Fabiana Laura, Paz, Dante Agustin, Jawerbaum, Alicia Sandra
Publicado:	2002
Materias:	Decidualization Maternal-embryo interaction Pregnancy Rat uterus gelatinase A nitric oxide nitric oxide donor nitric oxide synthase inhibitor tissue extract animal experiment animal tissue article assay blastocyst controlled study embryo endometrium enzyme activity female immunofluorescence test immunolocalization nidation nonhuman organ culture pregnancy priority journal protein expression rat rat strain uterine tube ligation uterus vagina smear Western blotting zymography Animals Blastocyst Embryo Implantation Enzyme Induction Enzyme Inhibitors Female Matrix Metalloproteinase 2 Nitric Oxide Nitric Oxide Donors Nitric Oxide Synthase omega-N-Methylarginine Organ Culture Techniques Rats Rats, Wistar Uterus
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00150282_v78_n6_p1278_Novaro http://hdl.handle.net/20.500.12110/paper_00150282_v78_n6_p1278_Novaro
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00150282_v78_n6_p1278_Novaro
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spelling	paper:paper_00150282_v78_n6_p1278_Novaro2023-06-08T14:37:54Z Nitric oxide induces gelatinase A (matrix metalloproteinase 2) during rat embryo implantation Novaro, Virginia Pustovrh, Maria Carolina Colman Lerner, Alejandro Ariel Lo Nostro, Fabiana Laura Paz, Dante Agustin Jawerbaum, Alicia Sandra Decidualization Maternal-embryo interaction Pregnancy Rat uterus gelatinase A nitric oxide nitric oxide donor nitric oxide synthase inhibitor tissue extract animal experiment animal tissue article assay blastocyst controlled study embryo endometrium enzyme activity female immunofluorescence test immunolocalization nidation nonhuman organ culture pregnancy priority journal protein expression rat rat strain uterine tube ligation uterus vagina smear Western blotting zymography Animals Blastocyst Embryo Implantation Enzyme Induction Enzyme Inhibitors Female Matrix Metalloproteinase 2 Nitric Oxide Nitric Oxide Donors Nitric Oxide Synthase omega-N-Methylarginine Organ Culture Techniques Pregnancy Rats Rats, Wistar Uterus Objective: To evaluate a reciprocal signaling interaction initiated by embryo-derived nitric oxide (NO) to facilitate implantation by increased production of gelatinase A (matrix metalloproteinase 2, MMP2) in uterine stroma. Design: Experimental animal studies. Setting: Reproductive-physiology research laboratory. Animal(s): Female syngeneic Wistar rats aged 14 weeks. Intervention(s): Vaginal smears to confirm pregnancy. Oviductal ligature to avoid the descent of blastocysts to the uterine lumen. Plasma exudation assays to locate uterine blastocyst implantation sites. Organ cultures treated with NO donors and nitric oxide synthase (NOS) inhibitors. Main Outcome Measure(s): Expression of MMP2 and NO was assessed by Western blot and zymography of tissue extracts and by immunofluorescence of tissue sections. Result(s): An increase in MMP2 activity was found in uterine extracts in early pregnant rats and was concentrated at implantation sites. Immunolocalization experiments showed that inducible NOS was expressed on the surface of the implanting blastocyst adjacent to the uterine epithelium at the sites of increased MMP2 expression. In organ culture experiments, NO donors were found to increase, whereas NOS inhibitors were found to decrease MMP2 activity in uterine tissue sections. Conclusion(s): Blastocyst-derived NO contributes to the production of uterine-derived MMP2, an essential component of implantation and initiation of placentation. © 2002 by American Society for Reproductive Medicine. Fil:Novaro, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pustovrh, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Colman-Lerner, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lo Nostro, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Paz, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Jawerbaum, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2002 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00150282_v78_n6_p1278_Novaro http://hdl.handle.net/20.500.12110/paper_00150282_v78_n6_p1278_Novaro
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Decidualization Maternal-embryo interaction Pregnancy Rat uterus gelatinase A nitric oxide nitric oxide donor nitric oxide synthase inhibitor tissue extract animal experiment animal tissue article assay blastocyst controlled study embryo endometrium enzyme activity female immunofluorescence test immunolocalization nidation nonhuman organ culture pregnancy priority journal protein expression rat rat strain uterine tube ligation uterus vagina smear Western blotting zymography Animals Blastocyst Embryo Implantation Enzyme Induction Enzyme Inhibitors Female Matrix Metalloproteinase 2 Nitric Oxide Nitric Oxide Donors Nitric Oxide Synthase omega-N-Methylarginine Organ Culture Techniques Pregnancy Rats Rats, Wistar Uterus
spellingShingle	Decidualization Maternal-embryo interaction Pregnancy Rat uterus gelatinase A nitric oxide nitric oxide donor nitric oxide synthase inhibitor tissue extract animal experiment animal tissue article assay blastocyst controlled study embryo endometrium enzyme activity female immunofluorescence test immunolocalization nidation nonhuman organ culture pregnancy priority journal protein expression rat rat strain uterine tube ligation uterus vagina smear Western blotting zymography Animals Blastocyst Embryo Implantation Enzyme Induction Enzyme Inhibitors Female Matrix Metalloproteinase 2 Nitric Oxide Nitric Oxide Donors Nitric Oxide Synthase omega-N-Methylarginine Organ Culture Techniques Pregnancy Rats Rats, Wistar Uterus Novaro, Virginia Pustovrh, Maria Carolina Colman Lerner, Alejandro Ariel Lo Nostro, Fabiana Laura Paz, Dante Agustin Jawerbaum, Alicia Sandra Nitric oxide induces gelatinase A (matrix metalloproteinase 2) during rat embryo implantation
topic_facet	Decidualization Maternal-embryo interaction Pregnancy Rat uterus gelatinase A nitric oxide nitric oxide donor nitric oxide synthase inhibitor tissue extract animal experiment animal tissue article assay blastocyst controlled study embryo endometrium enzyme activity female immunofluorescence test immunolocalization nidation nonhuman organ culture pregnancy priority journal protein expression rat rat strain uterine tube ligation uterus vagina smear Western blotting zymography Animals Blastocyst Embryo Implantation Enzyme Induction Enzyme Inhibitors Female Matrix Metalloproteinase 2 Nitric Oxide Nitric Oxide Donors Nitric Oxide Synthase omega-N-Methylarginine Organ Culture Techniques Pregnancy Rats Rats, Wistar Uterus
description	Objective: To evaluate a reciprocal signaling interaction initiated by embryo-derived nitric oxide (NO) to facilitate implantation by increased production of gelatinase A (matrix metalloproteinase 2, MMP2) in uterine stroma. Design: Experimental animal studies. Setting: Reproductive-physiology research laboratory. Animal(s): Female syngeneic Wistar rats aged 14 weeks. Intervention(s): Vaginal smears to confirm pregnancy. Oviductal ligature to avoid the descent of blastocysts to the uterine lumen. Plasma exudation assays to locate uterine blastocyst implantation sites. Organ cultures treated with NO donors and nitric oxide synthase (NOS) inhibitors. Main Outcome Measure(s): Expression of MMP2 and NO was assessed by Western blot and zymography of tissue extracts and by immunofluorescence of tissue sections. Result(s): An increase in MMP2 activity was found in uterine extracts in early pregnant rats and was concentrated at implantation sites. Immunolocalization experiments showed that inducible NOS was expressed on the surface of the implanting blastocyst adjacent to the uterine epithelium at the sites of increased MMP2 expression. In organ culture experiments, NO donors were found to increase, whereas NOS inhibitors were found to decrease MMP2 activity in uterine tissue sections. Conclusion(s): Blastocyst-derived NO contributes to the production of uterine-derived MMP2, an essential component of implantation and initiation of placentation. © 2002 by American Society for Reproductive Medicine.
author	Novaro, Virginia Pustovrh, Maria Carolina Colman Lerner, Alejandro Ariel Lo Nostro, Fabiana Laura Paz, Dante Agustin Jawerbaum, Alicia Sandra
author_facet	Novaro, Virginia Pustovrh, Maria Carolina Colman Lerner, Alejandro Ariel Lo Nostro, Fabiana Laura Paz, Dante Agustin Jawerbaum, Alicia Sandra
author_sort	Novaro, Virginia
title	Nitric oxide induces gelatinase A (matrix metalloproteinase 2) during rat embryo implantation
title_short	Nitric oxide induces gelatinase A (matrix metalloproteinase 2) during rat embryo implantation
title_full	Nitric oxide induces gelatinase A (matrix metalloproteinase 2) during rat embryo implantation
title_fullStr	Nitric oxide induces gelatinase A (matrix metalloproteinase 2) during rat embryo implantation
title_full_unstemmed	Nitric oxide induces gelatinase A (matrix metalloproteinase 2) during rat embryo implantation
title_sort	nitric oxide induces gelatinase a (matrix metalloproteinase 2) during rat embryo implantation
publishDate	2002
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00150282_v78_n6_p1278_Novaro http://hdl.handle.net/20.500.12110/paper_00150282_v78_n6_p1278_Novaro
work_keys_str_mv	AT novarovirginia nitricoxideinducesgelatinaseamatrixmetalloproteinase2duringratembryoimplantation AT pustovrhmariacarolina nitricoxideinducesgelatinaseamatrixmetalloproteinase2duringratembryoimplantation AT colmanlerneralejandroariel nitricoxideinducesgelatinaseamatrixmetalloproteinase2duringratembryoimplantation AT lonostrofabianalaura nitricoxideinducesgelatinaseamatrixmetalloproteinase2duringratembryoimplantation AT pazdanteagustin nitricoxideinducesgelatinaseamatrixmetalloproteinase2duringratembryoimplantation AT jawerbaumaliciasandra nitricoxideinducesgelatinaseamatrixmetalloproteinase2duringratembryoimplantation
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Nitric oxide induces gelatinase A (matrix metalloproteinase 2) during rat embryo implantation

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