Apoptosis and expression of Bcl-2 and Bax in eutopic endometrium from women with endometriosis

Objective: To evaluate and compare spontaneous apoptosis and Bcl-2 and Bax expression in eutopic endometrium from women with and without endometriosis. Design: Apoptosis and Bcl-2 and Bax expression were examined in eutopic endometrium from women with and without endometriosis. Setting: Instituto de...

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Guardado en:
Detalles Bibliográficos
Publicado: 2000
Materias:
Apoptosis
Bax
Bcl-2
Endometriosis
Eutopic endometrium
protein Bax
protein bcl 2
apoptosis
article
clinical article
controlled study
endometriosis
female
human
human tissue
immunohistochemistry
laparoscopy
priority journal
protein expression
Endometrium
Female
Human
In Situ Hybridization
Menstrual Cycle
Proto-Oncogene Proteins
Proto-Oncogene Proteins c-bcl-2
Support, Non-U.S. Gov't
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00150282_v74_n4_p760_Meresman
http://hdl.handle.net/20.500.12110/paper_00150282_v74_n4_p760_Meresman
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:Objective: To evaluate and compare spontaneous apoptosis and Bcl-2 and Bax expression in eutopic endometrium from women with and without endometriosis. Design: Apoptosis and Bcl-2 and Bax expression were examined in eutopic endometrium from women with and without endometriosis. Setting: Instituto de Biologia y Medicina Experimental-CONICET, Department of Gynecology and Department of Gynecological Pathology, Clinicas University Hospital, Buenos Aires, Argentina. Patient(s): Women with untreated endometriosis (n = 14) and controls (n = 16). Intervention(s): Collection of endometrial samples during diagnostic or therapeutic laparoscopy. Main Outcome Measure(s): Apoptotic cells were detected with use of the dUTP nick- end labeling (TUNEL) assay; Bcl-2 and Bax expressions were assessed with use of immunohistochemical techniques. Result(s): Spontaneous apoptosis was significantly lower in eutopic endometrium from patients with endometriosis, compared with healthy controls (2.26 ± 0.53 and 9.37 ± 1.69 apoptotic cells/field, respectively) and was independent of cycle phase. An increased expression of Bcl-2 protein was found in proliferative eutopic endometrium from patients with endometriosis. Bax expression was absent in proliferative endometrium, whereas there was an increase in its expression in secretory endometrium from both patients and controls. Conclusion(s): Women with endometriosis show decreased number of apoptotic cells in eutopic endometrium. The abnormal survival of endometrial cells may result in their continuing growth into ectopic locations. (C) 2000 by American Society for Reproductive Medicine.

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