Apoptosis and expression of Bcl-2 and Bax in eutopic endometrium from women with endometriosis

Objective: To evaluate and compare spontaneous apoptosis and Bcl-2 and Bax expression in eutopic endometrium from women with and without endometriosis. Design: Apoptosis and Bcl-2 and Bax expression were examined in eutopic endometrium from women with and without endometriosis. Setting: Instituto de...

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Publicado: 2000
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Bax
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00150282_v74_n4_p760_Meresman
http://hdl.handle.net/20.500.12110/paper_00150282_v74_n4_p760_Meresman
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spelling paper:paper_00150282_v74_n4_p760_Meresman2023-06-08T14:37:53Z Apoptosis and expression of Bcl-2 and Bax in eutopic endometrium from women with endometriosis Apoptosis Bax Bcl-2 Endometriosis Eutopic endometrium protein Bax protein bcl 2 apoptosis article clinical article controlled study endometriosis female human human tissue immunohistochemistry laparoscopy priority journal protein expression Apoptosis Endometriosis Endometrium Female Human In Situ Hybridization Menstrual Cycle Proto-Oncogene Proteins Proto-Oncogene Proteins c-bcl-2 Support, Non-U.S. Gov't Objective: To evaluate and compare spontaneous apoptosis and Bcl-2 and Bax expression in eutopic endometrium from women with and without endometriosis. Design: Apoptosis and Bcl-2 and Bax expression were examined in eutopic endometrium from women with and without endometriosis. Setting: Instituto de Biologia y Medicina Experimental-CONICET, Department of Gynecology and Department of Gynecological Pathology, Clinicas University Hospital, Buenos Aires, Argentina. Patient(s): Women with untreated endometriosis (n = 14) and controls (n = 16). Intervention(s): Collection of endometrial samples during diagnostic or therapeutic laparoscopy. Main Outcome Measure(s): Apoptotic cells were detected with use of the dUTP nick- end labeling (TUNEL) assay; Bcl-2 and Bax expressions were assessed with use of immunohistochemical techniques. Result(s): Spontaneous apoptosis was significantly lower in eutopic endometrium from patients with endometriosis, compared with healthy controls (2.26 ± 0.53 and 9.37 ± 1.69 apoptotic cells/field, respectively) and was independent of cycle phase. An increased expression of Bcl-2 protein was found in proliferative eutopic endometrium from patients with endometriosis. Bax expression was absent in proliferative endometrium, whereas there was an increase in its expression in secretory endometrium from both patients and controls. Conclusion(s): Women with endometriosis show decreased number of apoptotic cells in eutopic endometrium. The abnormal survival of endometrial cells may result in their continuing growth into ectopic locations. (C) 2000 by American Society for Reproductive Medicine. 2000 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00150282_v74_n4_p760_Meresman http://hdl.handle.net/20.500.12110/paper_00150282_v74_n4_p760_Meresman
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Apoptosis
Bax
Bcl-2
Endometriosis
Eutopic endometrium
protein Bax
protein bcl 2
apoptosis
article
clinical article
controlled study
endometriosis
female
human
human tissue
immunohistochemistry
laparoscopy
priority journal
protein expression
Apoptosis
Endometriosis
Endometrium
Female
Human
In Situ Hybridization
Menstrual Cycle
Proto-Oncogene Proteins
Proto-Oncogene Proteins c-bcl-2
Support, Non-U.S. Gov't
spellingShingle Apoptosis
Bax
Bcl-2
Endometriosis
Eutopic endometrium
protein Bax
protein bcl 2
apoptosis
article
clinical article
controlled study
endometriosis
female
human
human tissue
immunohistochemistry
laparoscopy
priority journal
protein expression
Apoptosis
Endometriosis
Endometrium
Female
Human
In Situ Hybridization
Menstrual Cycle
Proto-Oncogene Proteins
Proto-Oncogene Proteins c-bcl-2
Support, Non-U.S. Gov't
Apoptosis and expression of Bcl-2 and Bax in eutopic endometrium from women with endometriosis
topic_facet Apoptosis
Bax
Bcl-2
Endometriosis
Eutopic endometrium
protein Bax
protein bcl 2
apoptosis
article
clinical article
controlled study
endometriosis
female
human
human tissue
immunohistochemistry
laparoscopy
priority journal
protein expression
Apoptosis
Endometriosis
Endometrium
Female
Human
In Situ Hybridization
Menstrual Cycle
Proto-Oncogene Proteins
Proto-Oncogene Proteins c-bcl-2
Support, Non-U.S. Gov't
description Objective: To evaluate and compare spontaneous apoptosis and Bcl-2 and Bax expression in eutopic endometrium from women with and without endometriosis. Design: Apoptosis and Bcl-2 and Bax expression were examined in eutopic endometrium from women with and without endometriosis. Setting: Instituto de Biologia y Medicina Experimental-CONICET, Department of Gynecology and Department of Gynecological Pathology, Clinicas University Hospital, Buenos Aires, Argentina. Patient(s): Women with untreated endometriosis (n = 14) and controls (n = 16). Intervention(s): Collection of endometrial samples during diagnostic or therapeutic laparoscopy. Main Outcome Measure(s): Apoptotic cells were detected with use of the dUTP nick- end labeling (TUNEL) assay; Bcl-2 and Bax expressions were assessed with use of immunohistochemical techniques. Result(s): Spontaneous apoptosis was significantly lower in eutopic endometrium from patients with endometriosis, compared with healthy controls (2.26 ± 0.53 and 9.37 ± 1.69 apoptotic cells/field, respectively) and was independent of cycle phase. An increased expression of Bcl-2 protein was found in proliferative eutopic endometrium from patients with endometriosis. Bax expression was absent in proliferative endometrium, whereas there was an increase in its expression in secretory endometrium from both patients and controls. Conclusion(s): Women with endometriosis show decreased number of apoptotic cells in eutopic endometrium. The abnormal survival of endometrial cells may result in their continuing growth into ectopic locations. (C) 2000 by American Society for Reproductive Medicine.
title Apoptosis and expression of Bcl-2 and Bax in eutopic endometrium from women with endometriosis
title_short Apoptosis and expression of Bcl-2 and Bax in eutopic endometrium from women with endometriosis
title_full Apoptosis and expression of Bcl-2 and Bax in eutopic endometrium from women with endometriosis
title_fullStr Apoptosis and expression of Bcl-2 and Bax in eutopic endometrium from women with endometriosis
title_full_unstemmed Apoptosis and expression of Bcl-2 and Bax in eutopic endometrium from women with endometriosis
title_sort apoptosis and expression of bcl-2 and bax in eutopic endometrium from women with endometriosis
publishDate 2000
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00150282_v74_n4_p760_Meresman
http://hdl.handle.net/20.500.12110/paper_00150282_v74_n4_p760_Meresman
_version_ 1768541968364732416