Antibody response to a viral transcriptional regulator
The E2 transcriptional activator of the human papillomavirus regulates the expression of most viral transcripts. Its binding to specific target DNA sequences involves large conformational changes in the interacting macromolecules. The high stability of the E2:DNA complex prompted us to analyze the r...
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2003
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00145793_v534_n1-3_p202_Cerutti http://hdl.handle.net/20.500.12110/paper_00145793_v534_n1-3_p202_Cerutti |
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paper:paper_00145793_v534_n1-3_p202_Cerutti2023-06-08T14:37:42Z Antibody response to a viral transcriptional regulator Cerutti, María Laura de Prat Gay, Gonzalo Adjuvant Antibody Antigen binding Conformational epitope Transcription factor adjuvant epitope monoclonal antibody transcription factor transcription factor E2 unclassified drug antibody response antibody specificity article DNA binding DNA determination DNA sequence emulsion enzyme regulation enzyme stability epitope mapping humoral immunity immunization macromolecule molecular recognition nonhuman Papilloma virus priority journal protein conformation protein interaction transcription initiation transcription regulation virus expression virus transcription Human papillomavirus Papillomavirus The E2 transcriptional activator of the human papillomavirus regulates the expression of most viral transcripts. Its binding to specific target DNA sequences involves large conformational changes in the interacting macromolecules. The high stability of the E2:DNA complex prompted us to analyze the role of macromolecular interactions and adjuvant emulsions in the appearance of conformation-specific antibodies. We demonstrate that immunization with free or DNA-complexed E2 emulsified in an oil-in-water adjuvant elicits a humoral response shifted to the recognition of discontinuous epitopes. Epitope mapping and functional analysis of the generated anti-E2 mAbs reveals that two separate antibodies populations can be obtained: those able to form a stable ternary complex with protein and DNA, and those which recognize the DNA-binding surface of the transcription factor, interfering with E2 binding to DNA. © 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved. Fil:Cerutti, M.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:De Prat-Gay, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2003 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00145793_v534_n1-3_p202_Cerutti http://hdl.handle.net/20.500.12110/paper_00145793_v534_n1-3_p202_Cerutti |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Adjuvant Antibody Antigen binding Conformational epitope Transcription factor adjuvant epitope monoclonal antibody transcription factor transcription factor E2 unclassified drug antibody response antibody specificity article DNA binding DNA determination DNA sequence emulsion enzyme regulation enzyme stability epitope mapping humoral immunity immunization macromolecule molecular recognition nonhuman Papilloma virus priority journal protein conformation protein interaction transcription initiation transcription regulation virus expression virus transcription Human papillomavirus Papillomavirus |
| spellingShingle |
Adjuvant Antibody Antigen binding Conformational epitope Transcription factor adjuvant epitope monoclonal antibody transcription factor transcription factor E2 unclassified drug antibody response antibody specificity article DNA binding DNA determination DNA sequence emulsion enzyme regulation enzyme stability epitope mapping humoral immunity immunization macromolecule molecular recognition nonhuman Papilloma virus priority journal protein conformation protein interaction transcription initiation transcription regulation virus expression virus transcription Human papillomavirus Papillomavirus Cerutti, María Laura de Prat Gay, Gonzalo Antibody response to a viral transcriptional regulator |
| topic_facet |
Adjuvant Antibody Antigen binding Conformational epitope Transcription factor adjuvant epitope monoclonal antibody transcription factor transcription factor E2 unclassified drug antibody response antibody specificity article DNA binding DNA determination DNA sequence emulsion enzyme regulation enzyme stability epitope mapping humoral immunity immunization macromolecule molecular recognition nonhuman Papilloma virus priority journal protein conformation protein interaction transcription initiation transcription regulation virus expression virus transcription Human papillomavirus Papillomavirus |
| description |
The E2 transcriptional activator of the human papillomavirus regulates the expression of most viral transcripts. Its binding to specific target DNA sequences involves large conformational changes in the interacting macromolecules. The high stability of the E2:DNA complex prompted us to analyze the role of macromolecular interactions and adjuvant emulsions in the appearance of conformation-specific antibodies. We demonstrate that immunization with free or DNA-complexed E2 emulsified in an oil-in-water adjuvant elicits a humoral response shifted to the recognition of discontinuous epitopes. Epitope mapping and functional analysis of the generated anti-E2 mAbs reveals that two separate antibodies populations can be obtained: those able to form a stable ternary complex with protein and DNA, and those which recognize the DNA-binding surface of the transcription factor, interfering with E2 binding to DNA. © 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved. |
| author |
Cerutti, María Laura de Prat Gay, Gonzalo |
| author_facet |
Cerutti, María Laura de Prat Gay, Gonzalo |
| author_sort |
Cerutti, María Laura |
| title |
Antibody response to a viral transcriptional regulator |
| title_short |
Antibody response to a viral transcriptional regulator |
| title_full |
Antibody response to a viral transcriptional regulator |
| title_fullStr |
Antibody response to a viral transcriptional regulator |
| title_full_unstemmed |
Antibody response to a viral transcriptional regulator |
| title_sort |
antibody response to a viral transcriptional regulator |
| publishDate |
2003 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00145793_v534_n1-3_p202_Cerutti http://hdl.handle.net/20.500.12110/paper_00145793_v534_n1-3_p202_Cerutti |
| work_keys_str_mv |
AT ceruttimarialaura antibodyresponsetoaviraltranscriptionalregulator AT depratgaygonzalo antibodyresponsetoaviraltranscriptionalregulator |
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1768543929332924416 |