Arginine metabolism in Trypanosoma cruzi is coupled to parasite stage and replication
L-Arginine plays an essential role in the energetic metabolism of Trypanosoma cruzi. In this work we propose a relationship between L-arginine uptake, arginine kinase activity and the parasite replication ability. In epimastigote cultures L-arginine uptake decreases continuously accompanying a cell...
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2002
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00145793_v526_n1-3_p111_Pereira http://hdl.handle.net/20.500.12110/paper_00145793_v526_n1-3_p111_Pereira |
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paper:paper_00145793_v526_n1-3_p111_Pereira2023-06-08T14:37:41Z Arginine metabolism in Trypanosoma cruzi is coupled to parasite stage and replication Arginine kinase Arginine transport Arginine uptake Phosphoarginine Trypanosoma cruzi adenosine triphosphate antimitotic agent arginine arginine kinase hydroxyurea amino acid metabolism article cell division enzyme activity epimastigote life cycle nonhuman polyacrylamide gel electrophoresis priority journal synthesis Trypanosoma cruzi trypomastigote Western blotting Animals Arginine Arginine Kinase Biological Transport Life Cycle Stages Trypanosoma cruzi Trypanosoma Trypanosoma cruzi L-Arginine plays an essential role in the energetic metabolism of Trypanosoma cruzi. In this work we propose a relationship between L-arginine uptake, arginine kinase activity and the parasite replication ability. In epimastigote cultures L-arginine uptake decreases continuously accompanying a cell replication rate reduction. The use of conditioned or fresh medium mimics uptake variations. Interestingly, in non-replicative trypomastigote cells, L-arginine uptake was undetectable. The association between L-arginine uptake and cell replication was demonstrated using the antimitotic agent hydroxyurea. Arginine kinase, the enzyme responsible for phosphoarginine and ATP synthesis, also shows a differential activity in epimastigote and trypomastigote parasite stages. © 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies. 2002 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00145793_v526_n1-3_p111_Pereira http://hdl.handle.net/20.500.12110/paper_00145793_v526_n1-3_p111_Pereira |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Arginine kinase Arginine transport Arginine uptake Phosphoarginine Trypanosoma cruzi adenosine triphosphate antimitotic agent arginine arginine kinase hydroxyurea amino acid metabolism article cell division enzyme activity epimastigote life cycle nonhuman polyacrylamide gel electrophoresis priority journal synthesis Trypanosoma cruzi trypomastigote Western blotting Animals Arginine Arginine Kinase Biological Transport Life Cycle Stages Trypanosoma cruzi Trypanosoma Trypanosoma cruzi |
| spellingShingle |
Arginine kinase Arginine transport Arginine uptake Phosphoarginine Trypanosoma cruzi adenosine triphosphate antimitotic agent arginine arginine kinase hydroxyurea amino acid metabolism article cell division enzyme activity epimastigote life cycle nonhuman polyacrylamide gel electrophoresis priority journal synthesis Trypanosoma cruzi trypomastigote Western blotting Animals Arginine Arginine Kinase Biological Transport Life Cycle Stages Trypanosoma cruzi Trypanosoma Trypanosoma cruzi Arginine metabolism in Trypanosoma cruzi is coupled to parasite stage and replication |
| topic_facet |
Arginine kinase Arginine transport Arginine uptake Phosphoarginine Trypanosoma cruzi adenosine triphosphate antimitotic agent arginine arginine kinase hydroxyurea amino acid metabolism article cell division enzyme activity epimastigote life cycle nonhuman polyacrylamide gel electrophoresis priority journal synthesis Trypanosoma cruzi trypomastigote Western blotting Animals Arginine Arginine Kinase Biological Transport Life Cycle Stages Trypanosoma cruzi Trypanosoma Trypanosoma cruzi |
| description |
L-Arginine plays an essential role in the energetic metabolism of Trypanosoma cruzi. In this work we propose a relationship between L-arginine uptake, arginine kinase activity and the parasite replication ability. In epimastigote cultures L-arginine uptake decreases continuously accompanying a cell replication rate reduction. The use of conditioned or fresh medium mimics uptake variations. Interestingly, in non-replicative trypomastigote cells, L-arginine uptake was undetectable. The association between L-arginine uptake and cell replication was demonstrated using the antimitotic agent hydroxyurea. Arginine kinase, the enzyme responsible for phosphoarginine and ATP synthesis, also shows a differential activity in epimastigote and trypomastigote parasite stages. © 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies. |
| title |
Arginine metabolism in Trypanosoma cruzi is coupled to parasite stage and replication |
| title_short |
Arginine metabolism in Trypanosoma cruzi is coupled to parasite stage and replication |
| title_full |
Arginine metabolism in Trypanosoma cruzi is coupled to parasite stage and replication |
| title_fullStr |
Arginine metabolism in Trypanosoma cruzi is coupled to parasite stage and replication |
| title_full_unstemmed |
Arginine metabolism in Trypanosoma cruzi is coupled to parasite stage and replication |
| title_sort |
arginine metabolism in trypanosoma cruzi is coupled to parasite stage and replication |
| publishDate |
2002 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00145793_v526_n1-3_p111_Pereira http://hdl.handle.net/20.500.12110/paper_00145793_v526_n1-3_p111_Pereira |
| _version_ |
1768542869599027200 |