Cyclic nucleotide phosphodiesterase activity in Neurospora crassa Purification by immunoaffinity chromatography and characterization

Monoclonal antibodies to Neurospora crassa cyclic nucleotide phosphodiesterase (PDE I) were selected by their capacity to inhibit the enzyme activity. The monoclonal immunoglobulin, coupled to Sepharose 4B, was used for the affinity purification of PDE I activity. After SDS-polyacrylamide gel electr...

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Autores principales:	Rubinstein, Clara Patricia, Molina y Vedia, Luis Miguel, Torres, Héctor Norberto
Publicado:	1988
Materias:	(Neurospora crassa) Cyclic AMP phosphodiesterase Immunoaffinity chromatography Monoclonal antibody cyclic amp phosphodiesterase monoclonal antibody enzyme purification fungus immunoaffinity chromatography neurospora crassa nonhuman priority journal 3',5'-Cyclic-Nucleotide Phosphodiesterase Antibodies, Monoclonal Chromatography, Affinity Immunoglobulins, Heavy-Chain Immunoglobulins, Light-Chain Kinetics Molecular Weight Neurospora Neurospora crassa
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00145793_v241_n1-2_p219_Ulloa http://hdl.handle.net/20.500.12110/paper_00145793_v241_n1-2_p219_Ulloa
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00145793_v241_n1-2_p219_Ulloa
record_format	dspace
spelling	paper:paper_00145793_v241_n1-2_p219_Ulloa2023-06-08T14:37:33Z Cyclic nucleotide phosphodiesterase activity in Neurospora crassa Purification by immunoaffinity chromatography and characterization Rubinstein, Clara Patricia Molina y Vedia, Luis Miguel Torres, Héctor Norberto (Neurospora crassa) Cyclic AMP phosphodiesterase Immunoaffinity chromatography Monoclonal antibody cyclic amp phosphodiesterase monoclonal antibody enzyme purification fungus immunoaffinity chromatography neurospora crassa nonhuman priority journal 3',5'-Cyclic-Nucleotide Phosphodiesterase Antibodies, Monoclonal Chromatography, Affinity Immunoglobulins, Heavy-Chain Immunoglobulins, Light-Chain Kinetics Molecular Weight Neurospora Neurospora crassa Monoclonal antibodies to Neurospora crassa cyclic nucleotide phosphodiesterase (PDE I) were selected by their capacity to inhibit the enzyme activity. The monoclonal immunoglobulin, coupled to Sepharose 4B, was used for the affinity purification of PDE I activity. After SDS-polyacrylamide gel electrophoresis the affinity purified PDE I fractions showed a single polypeptide band of about 41 kDa. This band reacted in Western blots with the above mentioned monoclonal immunoglobulin. © 1988. Fil:Rubinstein, C.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Molina y Vedia, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Torres, H.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1988 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00145793_v241_n1-2_p219_Ulloa http://hdl.handle.net/20.500.12110/paper_00145793_v241_n1-2_p219_Ulloa
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	(Neurospora crassa) Cyclic AMP phosphodiesterase Immunoaffinity chromatography Monoclonal antibody cyclic amp phosphodiesterase monoclonal antibody enzyme purification fungus immunoaffinity chromatography neurospora crassa nonhuman priority journal 3',5'-Cyclic-Nucleotide Phosphodiesterase Antibodies, Monoclonal Chromatography, Affinity Immunoglobulins, Heavy-Chain Immunoglobulins, Light-Chain Kinetics Molecular Weight Neurospora Neurospora crassa
spellingShingle	(Neurospora crassa) Cyclic AMP phosphodiesterase Immunoaffinity chromatography Monoclonal antibody cyclic amp phosphodiesterase monoclonal antibody enzyme purification fungus immunoaffinity chromatography neurospora crassa nonhuman priority journal 3',5'-Cyclic-Nucleotide Phosphodiesterase Antibodies, Monoclonal Chromatography, Affinity Immunoglobulins, Heavy-Chain Immunoglobulins, Light-Chain Kinetics Molecular Weight Neurospora Neurospora crassa Rubinstein, Clara Patricia Molina y Vedia, Luis Miguel Torres, Héctor Norberto Cyclic nucleotide phosphodiesterase activity in Neurospora crassa Purification by immunoaffinity chromatography and characterization
topic_facet	(Neurospora crassa) Cyclic AMP phosphodiesterase Immunoaffinity chromatography Monoclonal antibody cyclic amp phosphodiesterase monoclonal antibody enzyme purification fungus immunoaffinity chromatography neurospora crassa nonhuman priority journal 3',5'-Cyclic-Nucleotide Phosphodiesterase Antibodies, Monoclonal Chromatography, Affinity Immunoglobulins, Heavy-Chain Immunoglobulins, Light-Chain Kinetics Molecular Weight Neurospora Neurospora crassa
description	Monoclonal antibodies to Neurospora crassa cyclic nucleotide phosphodiesterase (PDE I) were selected by their capacity to inhibit the enzyme activity. The monoclonal immunoglobulin, coupled to Sepharose 4B, was used for the affinity purification of PDE I activity. After SDS-polyacrylamide gel electrophoresis the affinity purified PDE I fractions showed a single polypeptide band of about 41 kDa. This band reacted in Western blots with the above mentioned monoclonal immunoglobulin. © 1988.
author	Rubinstein, Clara Patricia Molina y Vedia, Luis Miguel Torres, Héctor Norberto
author_facet	Rubinstein, Clara Patricia Molina y Vedia, Luis Miguel Torres, Héctor Norberto
author_sort	Rubinstein, Clara Patricia
title	Cyclic nucleotide phosphodiesterase activity in Neurospora crassa Purification by immunoaffinity chromatography and characterization
title_short	Cyclic nucleotide phosphodiesterase activity in Neurospora crassa Purification by immunoaffinity chromatography and characterization
title_full	Cyclic nucleotide phosphodiesterase activity in Neurospora crassa Purification by immunoaffinity chromatography and characterization
title_fullStr	Cyclic nucleotide phosphodiesterase activity in Neurospora crassa Purification by immunoaffinity chromatography and characterization
title_full_unstemmed	Cyclic nucleotide phosphodiesterase activity in Neurospora crassa Purification by immunoaffinity chromatography and characterization
title_sort	cyclic nucleotide phosphodiesterase activity in neurospora crassa purification by immunoaffinity chromatography and characterization
publishDate	1988
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00145793_v241_n1-2_p219_Ulloa http://hdl.handle.net/20.500.12110/paper_00145793_v241_n1-2_p219_Ulloa
work_keys_str_mv	AT rubinsteinclarapatricia cyclicnucleotidephosphodiesteraseactivityinneurosporacrassapurificationbyimmunoaffinitychromatographyandcharacterization AT molinayvedialuismiguel cyclicnucleotidephosphodiesteraseactivityinneurosporacrassapurificationbyimmunoaffinitychromatographyandcharacterization AT torreshectornorberto cyclicnucleotidephosphodiesteraseactivityinneurosporacrassapurificationbyimmunoaffinitychromatographyandcharacterization
_version_	1768543929148375040

Cyclic nucleotide phosphodiesterase activity in Neurospora crassa Purification by immunoaffinity chromatography and characterization

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