Trypanosoma cruzi: Analysis of the complete PUF RNA-binding protein family

The members of the PUF family of RNA-binding proteins regulate the fate of mRNAs by binding to their 3′UTR sequence elements in eukaryotes. In trypanosomes, for which gene expression is polycistronic and controlled almost exclusively by post-transcriptional processes, PUF proteins could play a cruci...

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Detalles Bibliográficos
Autores principales:	Caro, Florence, Bercovich, Natalia, Vazquez, Martín Pablo
Publicado:	2006
Materias:	aa amino acids Bioinformatics analysis Chagas disease hidden Markov model HMM nanos response element NRE open reading frame ORF Post-transcriptional regulation TriTryp Trypanosoma brucei, Trypanosoma cruzi, Leishmania major untranslated region UTR Yeast three hybrids mitochondrial protein protein protein kinase protein tcpuf1 protein tcpuf3 protein tcpuf5 protein tcpuf8 RNA binding protein unclassified drug article controlled study cytoplasm gene expression gene sequence genetic transcription genomics immunolocalization nonhuman nucleotide sequence prediction priority journal protein analysis protein family RNA binding RNA sequence Trypanosoma cruzi 3' Untranslated Regions Amino Acid Sequence Animals Base Sequence Mice Mice, Inbred BALB C Molecular Sequence Data Open Reading Frames Protozoan Proteins Repetitive Sequences, Amino Acid RNA-Binding Proteins Eukaryota Leishmania major Trypanosoma brucei
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00144894_v113_n2_p112_Caro http://hdl.handle.net/20.500.12110/paper_00144894_v113_n2_p112_Caro
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00144894_v113_n2_p112_Caro
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spelling	paper:paper_00144894_v113_n2_p112_Caro2023-06-08T14:37:22Z Trypanosoma cruzi: Analysis of the complete PUF RNA-binding protein family Caro, Florence Bercovich, Natalia Vazquez, Martín Pablo aa amino acids Bioinformatics analysis Chagas disease hidden Markov model HMM nanos response element NRE open reading frame ORF Post-transcriptional regulation TriTryp Trypanosoma brucei, Trypanosoma cruzi, Leishmania major untranslated region UTR Yeast three hybrids mitochondrial protein protein protein kinase protein tcpuf1 protein tcpuf3 protein tcpuf5 protein tcpuf8 RNA binding protein unclassified drug article controlled study cytoplasm gene expression gene sequence genetic transcription genomics immunolocalization nonhuman nucleotide sequence prediction priority journal protein analysis protein family RNA binding RNA sequence Trypanosoma cruzi 3' Untranslated Regions Amino Acid Sequence Animals Base Sequence Mice Mice, Inbred BALB C Molecular Sequence Data Open Reading Frames Protozoan Proteins Repetitive Sequences, Amino Acid RNA-Binding Proteins Trypanosoma cruzi Eukaryota Leishmania major Trypanosoma brucei Trypanosoma cruzi The members of the PUF family of RNA-binding proteins regulate the fate of mRNAs by binding to their 3′UTR sequence elements in eukaryotes. In trypanosomes, for which gene expression is polycistronic and controlled almost exclusively by post-transcriptional processes, PUF proteins could play a crucial role. We report here the complete analysis of the PUF protein family of Trypanosoma cruzi composed of 10 members. In silico analysis predicts the existence of at least three major groups within the T. cruzi family, based on their putative binding specificity. Using yeast three hybrid assays, we tested some of these predictions for TcPUF1, TcPUF3, TcPUF5, and TcPUF8 as representatives of these groups. Data mining of the T. cruzi genome led us to describe putative binding targets for the TcPUFs of the most conserved group, TcPUF1 and TcPUF2. The targets include genes for mitochondrial proteins and protein kinases. Finally, immunolocalization experiments showed that TcPUF1 is localized in multiple discrete foci in the cytoplasm supporting its proposed function. © 2005 Elsevier Inc. All rights reserved. Fil:Caro, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bercovich, N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Vázquez, M.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2006 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00144894_v113_n2_p112_Caro http://hdl.handle.net/20.500.12110/paper_00144894_v113_n2_p112_Caro
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	aa amino acids Bioinformatics analysis Chagas disease hidden Markov model HMM nanos response element NRE open reading frame ORF Post-transcriptional regulation TriTryp Trypanosoma brucei, Trypanosoma cruzi, Leishmania major untranslated region UTR Yeast three hybrids mitochondrial protein protein protein kinase protein tcpuf1 protein tcpuf3 protein tcpuf5 protein tcpuf8 RNA binding protein unclassified drug article controlled study cytoplasm gene expression gene sequence genetic transcription genomics immunolocalization nonhuman nucleotide sequence prediction priority journal protein analysis protein family RNA binding RNA sequence Trypanosoma cruzi 3' Untranslated Regions Amino Acid Sequence Animals Base Sequence Mice Mice, Inbred BALB C Molecular Sequence Data Open Reading Frames Protozoan Proteins Repetitive Sequences, Amino Acid RNA-Binding Proteins Trypanosoma cruzi Eukaryota Leishmania major Trypanosoma brucei Trypanosoma cruzi
spellingShingle	aa amino acids Bioinformatics analysis Chagas disease hidden Markov model HMM nanos response element NRE open reading frame ORF Post-transcriptional regulation TriTryp Trypanosoma brucei, Trypanosoma cruzi, Leishmania major untranslated region UTR Yeast three hybrids mitochondrial protein protein protein kinase protein tcpuf1 protein tcpuf3 protein tcpuf5 protein tcpuf8 RNA binding protein unclassified drug article controlled study cytoplasm gene expression gene sequence genetic transcription genomics immunolocalization nonhuman nucleotide sequence prediction priority journal protein analysis protein family RNA binding RNA sequence Trypanosoma cruzi 3' Untranslated Regions Amino Acid Sequence Animals Base Sequence Mice Mice, Inbred BALB C Molecular Sequence Data Open Reading Frames Protozoan Proteins Repetitive Sequences, Amino Acid RNA-Binding Proteins Trypanosoma cruzi Eukaryota Leishmania major Trypanosoma brucei Trypanosoma cruzi Caro, Florence Bercovich, Natalia Vazquez, Martín Pablo Trypanosoma cruzi: Analysis of the complete PUF RNA-binding protein family
topic_facet	aa amino acids Bioinformatics analysis Chagas disease hidden Markov model HMM nanos response element NRE open reading frame ORF Post-transcriptional regulation TriTryp Trypanosoma brucei, Trypanosoma cruzi, Leishmania major untranslated region UTR Yeast three hybrids mitochondrial protein protein protein kinase protein tcpuf1 protein tcpuf3 protein tcpuf5 protein tcpuf8 RNA binding protein unclassified drug article controlled study cytoplasm gene expression gene sequence genetic transcription genomics immunolocalization nonhuman nucleotide sequence prediction priority journal protein analysis protein family RNA binding RNA sequence Trypanosoma cruzi 3' Untranslated Regions Amino Acid Sequence Animals Base Sequence Mice Mice, Inbred BALB C Molecular Sequence Data Open Reading Frames Protozoan Proteins Repetitive Sequences, Amino Acid RNA-Binding Proteins Trypanosoma cruzi Eukaryota Leishmania major Trypanosoma brucei Trypanosoma cruzi
description	The members of the PUF family of RNA-binding proteins regulate the fate of mRNAs by binding to their 3′UTR sequence elements in eukaryotes. In trypanosomes, for which gene expression is polycistronic and controlled almost exclusively by post-transcriptional processes, PUF proteins could play a crucial role. We report here the complete analysis of the PUF protein family of Trypanosoma cruzi composed of 10 members. In silico analysis predicts the existence of at least three major groups within the T. cruzi family, based on their putative binding specificity. Using yeast three hybrid assays, we tested some of these predictions for TcPUF1, TcPUF3, TcPUF5, and TcPUF8 as representatives of these groups. Data mining of the T. cruzi genome led us to describe putative binding targets for the TcPUFs of the most conserved group, TcPUF1 and TcPUF2. The targets include genes for mitochondrial proteins and protein kinases. Finally, immunolocalization experiments showed that TcPUF1 is localized in multiple discrete foci in the cytoplasm supporting its proposed function. © 2005 Elsevier Inc. All rights reserved.
author	Caro, Florence Bercovich, Natalia Vazquez, Martín Pablo
author_facet	Caro, Florence Bercovich, Natalia Vazquez, Martín Pablo
author_sort	Caro, Florence
title	Trypanosoma cruzi: Analysis of the complete PUF RNA-binding protein family
title_short	Trypanosoma cruzi: Analysis of the complete PUF RNA-binding protein family
title_full	Trypanosoma cruzi: Analysis of the complete PUF RNA-binding protein family
title_fullStr	Trypanosoma cruzi: Analysis of the complete PUF RNA-binding protein family
title_full_unstemmed	Trypanosoma cruzi: Analysis of the complete PUF RNA-binding protein family
title_sort	trypanosoma cruzi: analysis of the complete puf rna-binding protein family
publishDate	2006
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00144894_v113_n2_p112_Caro http://hdl.handle.net/20.500.12110/paper_00144894_v113_n2_p112_Caro
work_keys_str_mv	AT caroflorence trypanosomacruzianalysisofthecompletepufrnabindingproteinfamily AT bercovichnatalia trypanosomacruzianalysisofthecompletepufrnabindingproteinfamily AT vazquezmartinpablo trypanosomacruzianalysisofthecompletepufrnabindingproteinfamily
_version_	1768546426936098816

Trypanosoma cruzi: Analysis of the complete PUF RNA-binding protein family

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