Remyelination after cuprizone-induced demyelination in the rat is stimulated by apotransferrin
Twenty-one-day-old Wistar rats were fed a diet containing 0.6% cuprizone for 2 weeks. Studies carried out after withdrawal of cuprizone showed histological evidences of marked demyelination in the corpus callosum. Biochemical studies of isolated myelin showed a marked decrease in myelin proteins, ph...
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2006
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00144886_v198_n2_p519_Adamo http://hdl.handle.net/20.500.12110/paper_00144886_v198_n2_p519_Adamo |
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paper:paper_00144886_v198_n2_p519_Adamo2023-06-08T14:37:20Z Remyelination after cuprizone-induced demyelination in the rat is stimulated by apotransferrin Apotransferrin Cuprizone Demyelinating diseases Demyelination Oligodendroglial cell differentiation Remyelination apotransferrin broxuridine cuprizone galactosylceramide myelin protein phospholipid animal experiment animal model animal tissue article chemical analysis controlled study corpus callosum demyelinating disease demyelination female histology immunocytochemistry male nonhuman priority journal protein expression protein isolation rat remyelinization statistical significance stem cell Analysis of Variance Animals Animals, Newborn Antigens Antigens, CD11b Apoproteins Brain Bromodeoxyuridine Cell Count Cuprizone Cytoskeletal Proteins Demyelinating Diseases Drug Interactions Galactolipids Glial Fibrillary Acidic Protein Immunohistochemistry Indoles Myelin Basic Proteins Myelin Sheath Proteoglycans Rats Rats, Wistar Recovery of Function Regeneration Time Factors Transferrin Twenty-one-day-old Wistar rats were fed a diet containing 0.6% cuprizone for 2 weeks. Studies carried out after withdrawal of cuprizone showed histological evidences of marked demyelination in the corpus callosum. Biochemical studies of isolated myelin showed a marked decrease in myelin proteins, phospholipids, and galactocerebrosides as well as a marked decrease in myelin yield. Treatment of these animals with a single intracranial injection of 350 ng of apotransferrin at the time of withdrawal of cuprizone induced a marked increase in myelin deposition resulting in a significantly improved remyelination, evaluated by histological, immunocytochemical, and biochemical parameters, in comparison to what was observed in spontaneous recovery. Immunocytochemical studies of cryotome sections to analyze developmental parameters of the oligodendroglial cell population at the time of termination of cuprizone and at different times thereafter showed that in the untreated animals, there was a marked increase in the number of NG2-BrdU-positive precursor cells together with a marked decrease in MBP expression at the peak of cuprizone-induced demyelination. As expected, the amount of precursor cells decreased markedly during spontaneous remyelination and was accompanied by an increase in MBP reactivity. In the apotransferrin-treated animals, these phenomena occurred much faster, and remyelination was much more efficient than in the untreated controls. The results of this study suggest that apotransferrin is a very active promyelinating agent which could be important for the treatment of certain demyelinating conditions. © 2006 Elsevier Inc. All rights reserved. 2006 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00144886_v198_n2_p519_Adamo http://hdl.handle.net/20.500.12110/paper_00144886_v198_n2_p519_Adamo |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Apotransferrin Cuprizone Demyelinating diseases Demyelination Oligodendroglial cell differentiation Remyelination apotransferrin broxuridine cuprizone galactosylceramide myelin protein phospholipid animal experiment animal model animal tissue article chemical analysis controlled study corpus callosum demyelinating disease demyelination female histology immunocytochemistry male nonhuman priority journal protein expression protein isolation rat remyelinization statistical significance stem cell Analysis of Variance Animals Animals, Newborn Antigens Antigens, CD11b Apoproteins Brain Bromodeoxyuridine Cell Count Cuprizone Cytoskeletal Proteins Demyelinating Diseases Drug Interactions Galactolipids Glial Fibrillary Acidic Protein Immunohistochemistry Indoles Myelin Basic Proteins Myelin Sheath Proteoglycans Rats Rats, Wistar Recovery of Function Regeneration Time Factors Transferrin |
| spellingShingle |
Apotransferrin Cuprizone Demyelinating diseases Demyelination Oligodendroglial cell differentiation Remyelination apotransferrin broxuridine cuprizone galactosylceramide myelin protein phospholipid animal experiment animal model animal tissue article chemical analysis controlled study corpus callosum demyelinating disease demyelination female histology immunocytochemistry male nonhuman priority journal protein expression protein isolation rat remyelinization statistical significance stem cell Analysis of Variance Animals Animals, Newborn Antigens Antigens, CD11b Apoproteins Brain Bromodeoxyuridine Cell Count Cuprizone Cytoskeletal Proteins Demyelinating Diseases Drug Interactions Galactolipids Glial Fibrillary Acidic Protein Immunohistochemistry Indoles Myelin Basic Proteins Myelin Sheath Proteoglycans Rats Rats, Wistar Recovery of Function Regeneration Time Factors Transferrin Remyelination after cuprizone-induced demyelination in the rat is stimulated by apotransferrin |
| topic_facet |
Apotransferrin Cuprizone Demyelinating diseases Demyelination Oligodendroglial cell differentiation Remyelination apotransferrin broxuridine cuprizone galactosylceramide myelin protein phospholipid animal experiment animal model animal tissue article chemical analysis controlled study corpus callosum demyelinating disease demyelination female histology immunocytochemistry male nonhuman priority journal protein expression protein isolation rat remyelinization statistical significance stem cell Analysis of Variance Animals Animals, Newborn Antigens Antigens, CD11b Apoproteins Brain Bromodeoxyuridine Cell Count Cuprizone Cytoskeletal Proteins Demyelinating Diseases Drug Interactions Galactolipids Glial Fibrillary Acidic Protein Immunohistochemistry Indoles Myelin Basic Proteins Myelin Sheath Proteoglycans Rats Rats, Wistar Recovery of Function Regeneration Time Factors Transferrin |
| description |
Twenty-one-day-old Wistar rats were fed a diet containing 0.6% cuprizone for 2 weeks. Studies carried out after withdrawal of cuprizone showed histological evidences of marked demyelination in the corpus callosum. Biochemical studies of isolated myelin showed a marked decrease in myelin proteins, phospholipids, and galactocerebrosides as well as a marked decrease in myelin yield. Treatment of these animals with a single intracranial injection of 350 ng of apotransferrin at the time of withdrawal of cuprizone induced a marked increase in myelin deposition resulting in a significantly improved remyelination, evaluated by histological, immunocytochemical, and biochemical parameters, in comparison to what was observed in spontaneous recovery. Immunocytochemical studies of cryotome sections to analyze developmental parameters of the oligodendroglial cell population at the time of termination of cuprizone and at different times thereafter showed that in the untreated animals, there was a marked increase in the number of NG2-BrdU-positive precursor cells together with a marked decrease in MBP expression at the peak of cuprizone-induced demyelination. As expected, the amount of precursor cells decreased markedly during spontaneous remyelination and was accompanied by an increase in MBP reactivity. In the apotransferrin-treated animals, these phenomena occurred much faster, and remyelination was much more efficient than in the untreated controls. The results of this study suggest that apotransferrin is a very active promyelinating agent which could be important for the treatment of certain demyelinating conditions. © 2006 Elsevier Inc. All rights reserved. |
| title |
Remyelination after cuprizone-induced demyelination in the rat is stimulated by apotransferrin |
| title_short |
Remyelination after cuprizone-induced demyelination in the rat is stimulated by apotransferrin |
| title_full |
Remyelination after cuprizone-induced demyelination in the rat is stimulated by apotransferrin |
| title_fullStr |
Remyelination after cuprizone-induced demyelination in the rat is stimulated by apotransferrin |
| title_full_unstemmed |
Remyelination after cuprizone-induced demyelination in the rat is stimulated by apotransferrin |
| title_sort |
remyelination after cuprizone-induced demyelination in the rat is stimulated by apotransferrin |
| publishDate |
2006 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00144886_v198_n2_p519_Adamo http://hdl.handle.net/20.500.12110/paper_00144886_v198_n2_p519_Adamo |
| _version_ |
1768545401833521152 |