Therapeutic action of meliacine, a plant-derived antiviral, on HSV-induced ocular disease in mice

Ocular herpes simplex virus type-1 (HSV-1) infections remain an important cause of corneal disease which may result in a loss of vision. Meliacine (MA), an antiviral activity present in crude leaf extracts of Melia azedarach L. that inhibits HSV-1 multiplication in vitro, was studied in a murine her...

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Autores principales:	Pifarré, María Paula, Coto, Celia Esther, Alche, Laura Edith
Publicado:	2002
Materias:	Herpes simplex virus In vivo Latency Melia azedarach L. Murine model Plant antiviral Stromal keratitis Melia azedarach extract meliacine plant extract unclassified drug animal cell animal experiment animal model animal tissue antiviral activity article controlled study disease severity Herpes simplex virus 1 histopathology immunomodulation inoculation keratitis microscopy mouse necrosis nonhuman priority journal vascularization
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00144835_v75_n3_p327_PaulaPifarre http://hdl.handle.net/20.500.12110/paper_00144835_v75_n3_p327_PaulaPifarre
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00144835_v75_n3_p327_PaulaPifarre
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spelling	paper:paper_00144835_v75_n3_p327_PaulaPifarre2023-06-08T14:37:19Z Therapeutic action of meliacine, a plant-derived antiviral, on HSV-induced ocular disease in mice Pifarré, María Paula Coto, Celia Esther Alche, Laura Edith Herpes simplex virus In vivo Latency Melia azedarach L. Murine model Plant antiviral Stromal keratitis Melia azedarach extract meliacine plant extract unclassified drug animal cell animal experiment animal model animal tissue antiviral activity article controlled study disease severity Herpes simplex virus 1 histopathology immunomodulation inoculation keratitis microscopy mouse necrosis nonhuman priority journal vascularization Ocular herpes simplex virus type-1 (HSV-1) infections remain an important cause of corneal disease which may result in a loss of vision. Meliacine (MA), an antiviral activity present in crude leaf extracts of Melia azedarach L. that inhibits HSV-1 multiplication in vitro, was studied in a murine herpetic stromal keratitis experimental model. Adult Balb/c mice were inoculated with HSV-1 at their corneas after abrasion. MA was administered topically three times a day for 3 consecutive days, beginning at 24 and 96 hr after infection. Infected animals treated or not with MA were monitored for the development of ocular disease by a binocular microscope for 16 days. MA significantly reduced the incidence and the severity of blepharitis, neovascularization and stromal keratitis with respect to untreated infected mice, regardless the schedule of treatment assayed. Histological examination of corneas from MA-treated animals revealed no tissue damage, whereas corneal samples from untreated infected mice showed inflammation, vascularization and necrosis. In uninfected mice treated with MA, we found no evidence of corneal damage and histopathological studies showed no changes in the corneas of these mice. Treatment with MA at 24 hours post-infection (h.p.i.) reduced viral multiplication in the eye by 1-1.5 orders of magnitude. Studies on latency revealed that MA sligthly affected the establishment of a latent infection. Thus, MA proved to exert an antiviral action on the development of herpetic stromal keratitis when supplied by post-treatment. Unexpectedly, treatment with MA after 96 h.p.i prevented ocular disease, suggesting an in vivo immunomodulating activity of MA. © 2002 Elsevier Science Ltd. Fil:Paula Pifarré, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Coto, C.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Alché, L.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2002 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00144835_v75_n3_p327_PaulaPifarre http://hdl.handle.net/20.500.12110/paper_00144835_v75_n3_p327_PaulaPifarre
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Herpes simplex virus In vivo Latency Melia azedarach L. Murine model Plant antiviral Stromal keratitis Melia azedarach extract meliacine plant extract unclassified drug animal cell animal experiment animal model animal tissue antiviral activity article controlled study disease severity Herpes simplex virus 1 histopathology immunomodulation inoculation keratitis microscopy mouse necrosis nonhuman priority journal vascularization
spellingShingle	Herpes simplex virus In vivo Latency Melia azedarach L. Murine model Plant antiviral Stromal keratitis Melia azedarach extract meliacine plant extract unclassified drug animal cell animal experiment animal model animal tissue antiviral activity article controlled study disease severity Herpes simplex virus 1 histopathology immunomodulation inoculation keratitis microscopy mouse necrosis nonhuman priority journal vascularization Pifarré, María Paula Coto, Celia Esther Alche, Laura Edith Therapeutic action of meliacine, a plant-derived antiviral, on HSV-induced ocular disease in mice
topic_facet	Herpes simplex virus In vivo Latency Melia azedarach L. Murine model Plant antiviral Stromal keratitis Melia azedarach extract meliacine plant extract unclassified drug animal cell animal experiment animal model animal tissue antiviral activity article controlled study disease severity Herpes simplex virus 1 histopathology immunomodulation inoculation keratitis microscopy mouse necrosis nonhuman priority journal vascularization
description	Ocular herpes simplex virus type-1 (HSV-1) infections remain an important cause of corneal disease which may result in a loss of vision. Meliacine (MA), an antiviral activity present in crude leaf extracts of Melia azedarach L. that inhibits HSV-1 multiplication in vitro, was studied in a murine herpetic stromal keratitis experimental model. Adult Balb/c mice were inoculated with HSV-1 at their corneas after abrasion. MA was administered topically three times a day for 3 consecutive days, beginning at 24 and 96 hr after infection. Infected animals treated or not with MA were monitored for the development of ocular disease by a binocular microscope for 16 days. MA significantly reduced the incidence and the severity of blepharitis, neovascularization and stromal keratitis with respect to untreated infected mice, regardless the schedule of treatment assayed. Histological examination of corneas from MA-treated animals revealed no tissue damage, whereas corneal samples from untreated infected mice showed inflammation, vascularization and necrosis. In uninfected mice treated with MA, we found no evidence of corneal damage and histopathological studies showed no changes in the corneas of these mice. Treatment with MA at 24 hours post-infection (h.p.i.) reduced viral multiplication in the eye by 1-1.5 orders of magnitude. Studies on latency revealed that MA sligthly affected the establishment of a latent infection. Thus, MA proved to exert an antiviral action on the development of herpetic stromal keratitis when supplied by post-treatment. Unexpectedly, treatment with MA after 96 h.p.i prevented ocular disease, suggesting an in vivo immunomodulating activity of MA. © 2002 Elsevier Science Ltd.
author	Pifarré, María Paula Coto, Celia Esther Alche, Laura Edith
author_facet	Pifarré, María Paula Coto, Celia Esther Alche, Laura Edith
author_sort	Pifarré, María Paula
title	Therapeutic action of meliacine, a plant-derived antiviral, on HSV-induced ocular disease in mice
title_short	Therapeutic action of meliacine, a plant-derived antiviral, on HSV-induced ocular disease in mice
title_full	Therapeutic action of meliacine, a plant-derived antiviral, on HSV-induced ocular disease in mice
title_fullStr	Therapeutic action of meliacine, a plant-derived antiviral, on HSV-induced ocular disease in mice
title_full_unstemmed	Therapeutic action of meliacine, a plant-derived antiviral, on HSV-induced ocular disease in mice
title_sort	therapeutic action of meliacine, a plant-derived antiviral, on hsv-induced ocular disease in mice
publishDate	2002
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00144835_v75_n3_p327_PaulaPifarre http://hdl.handle.net/20.500.12110/paper_00144835_v75_n3_p327_PaulaPifarre
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Therapeutic action of meliacine, a plant-derived antiviral, on HSV-induced ocular disease in mice

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