Effect of estradiol and tamoxifen on the anchorage-independent growth of the subpopulations derived from MCF-7 breast carcinoma cells: Cytogenetic analysis of the stem cell subpopulation

The MCF-7 breast carcinoma cell line can be separated by Percoll density gradient centrifugation into several subpopulations, A to F, one of which (E) has been previously suggested to be highly enriched in stem cells. The anchorage-independent growth of the different fractions and its sensitivity to...

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Autores principales:	Podhajcer, Osvaldo Luis, Resnicoff, Mariana, Bover, Laura del Carmen, Lauinger de Medrano, Estela E., Larripa, Irene Beatriz
Publicado:	1988
Materias:	estradiol tamoxifen anchorage independent growth cell culture cell growth cell strain mcf 7 controlled study cytology female human human cell karyotyping priority journal stem cell ultrastructure Breast Neoplasms Cell Adhesion Cell Division Cell Line Estradiol Genetic Markers Human Karyotyping Support, Non-U.S. Gov't Tamoxifen Tumor Stem Cells
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00144827_v179_n1_p58_Podhajcer http://hdl.handle.net/20.500.12110/paper_00144827_v179_n1_p58_Podhajcer
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00144827_v179_n1_p58_Podhajcer
record_format	dspace
spelling	paper:paper_00144827_v179_n1_p58_Podhajcer2023-06-08T14:37:15Z Effect of estradiol and tamoxifen on the anchorage-independent growth of the subpopulations derived from MCF-7 breast carcinoma cells: Cytogenetic analysis of the stem cell subpopulation Podhajcer, Osvaldo Luis Resnicoff, Mariana Bover, Laura del Carmen Lauinger de Medrano, Estela E. Larripa, Irene Beatriz estradiol tamoxifen anchorage independent growth cell culture cell growth cell strain mcf 7 controlled study cytology female human human cell karyotyping priority journal stem cell ultrastructure Breast Neoplasms Cell Adhesion Cell Division Cell Line Estradiol Genetic Markers Human Karyotyping Support, Non-U.S. Gov't Tamoxifen Tumor Stem Cells The MCF-7 breast carcinoma cell line can be separated by Percoll density gradient centrifugation into several subpopulations, A to F, one of which (E) has been previously suggested to be highly enriched in stem cells. The anchorage-independent growth of the different fractions and its sensitivity to estradiol (E2) and tamoxifen (TAM) was assayed. The anchorage-independent growth capacity of the different fractions was E>A>B> D>C,F. The E fraction had the highest clonogenic index (6.62 ± 1.18) and was unaffected by E2 or TAM. The karyotypic analysis of the E fraction revealed features similar to those of the unfractionated cell line. It is suggested that the high growth rate of fraction E is due to an enrichment in stem cells and not to the existence of a different clone. © 1988. Fil:Podhajcer, O.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Resnicoff, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bover, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Medrano, E.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Larripa, I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1988 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00144827_v179_n1_p58_Podhajcer http://hdl.handle.net/20.500.12110/paper_00144827_v179_n1_p58_Podhajcer
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	estradiol tamoxifen anchorage independent growth cell culture cell growth cell strain mcf 7 controlled study cytology female human human cell karyotyping priority journal stem cell ultrastructure Breast Neoplasms Cell Adhesion Cell Division Cell Line Estradiol Genetic Markers Human Karyotyping Support, Non-U.S. Gov't Tamoxifen Tumor Stem Cells
spellingShingle	estradiol tamoxifen anchorage independent growth cell culture cell growth cell strain mcf 7 controlled study cytology female human human cell karyotyping priority journal stem cell ultrastructure Breast Neoplasms Cell Adhesion Cell Division Cell Line Estradiol Genetic Markers Human Karyotyping Support, Non-U.S. Gov't Tamoxifen Tumor Stem Cells Podhajcer, Osvaldo Luis Resnicoff, Mariana Bover, Laura del Carmen Lauinger de Medrano, Estela E. Larripa, Irene Beatriz Effect of estradiol and tamoxifen on the anchorage-independent growth of the subpopulations derived from MCF-7 breast carcinoma cells: Cytogenetic analysis of the stem cell subpopulation
topic_facet	estradiol tamoxifen anchorage independent growth cell culture cell growth cell strain mcf 7 controlled study cytology female human human cell karyotyping priority journal stem cell ultrastructure Breast Neoplasms Cell Adhesion Cell Division Cell Line Estradiol Genetic Markers Human Karyotyping Support, Non-U.S. Gov't Tamoxifen Tumor Stem Cells
description	The MCF-7 breast carcinoma cell line can be separated by Percoll density gradient centrifugation into several subpopulations, A to F, one of which (E) has been previously suggested to be highly enriched in stem cells. The anchorage-independent growth of the different fractions and its sensitivity to estradiol (E2) and tamoxifen (TAM) was assayed. The anchorage-independent growth capacity of the different fractions was E>A>B> D>C,F. The E fraction had the highest clonogenic index (6.62 ± 1.18) and was unaffected by E2 or TAM. The karyotypic analysis of the E fraction revealed features similar to those of the unfractionated cell line. It is suggested that the high growth rate of fraction E is due to an enrichment in stem cells and not to the existence of a different clone. © 1988.
author	Podhajcer, Osvaldo Luis Resnicoff, Mariana Bover, Laura del Carmen Lauinger de Medrano, Estela E. Larripa, Irene Beatriz
author_facet	Podhajcer, Osvaldo Luis Resnicoff, Mariana Bover, Laura del Carmen Lauinger de Medrano, Estela E. Larripa, Irene Beatriz
author_sort	Podhajcer, Osvaldo Luis
title	Effect of estradiol and tamoxifen on the anchorage-independent growth of the subpopulations derived from MCF-7 breast carcinoma cells: Cytogenetic analysis of the stem cell subpopulation
title_short	Effect of estradiol and tamoxifen on the anchorage-independent growth of the subpopulations derived from MCF-7 breast carcinoma cells: Cytogenetic analysis of the stem cell subpopulation
title_full	Effect of estradiol and tamoxifen on the anchorage-independent growth of the subpopulations derived from MCF-7 breast carcinoma cells: Cytogenetic analysis of the stem cell subpopulation
title_fullStr	Effect of estradiol and tamoxifen on the anchorage-independent growth of the subpopulations derived from MCF-7 breast carcinoma cells: Cytogenetic analysis of the stem cell subpopulation
title_full_unstemmed	Effect of estradiol and tamoxifen on the anchorage-independent growth of the subpopulations derived from MCF-7 breast carcinoma cells: Cytogenetic analysis of the stem cell subpopulation
title_sort	effect of estradiol and tamoxifen on the anchorage-independent growth of the subpopulations derived from mcf-7 breast carcinoma cells: cytogenetic analysis of the stem cell subpopulation
publishDate	1988
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00144827_v179_n1_p58_Podhajcer http://hdl.handle.net/20.500.12110/paper_00144827_v179_n1_p58_Podhajcer
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_version_	1768543259902083072

Effect of estradiol and tamoxifen on the anchorage-independent growth of the subpopulations derived from MCF-7 breast carcinoma cells: Cytogenetic analysis of the stem cell subpopulation

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