Protein kinase C is differentially regulated by thrombin, insulin, and epidermal growth factor in human mammary tumor cells

The exposure of serum-deprived mammary tumor cells MCF-7 and T-47D to insulin, thrombin, and epidermal growth factor (EGF) resulted in dramatic modifications in the activity and in the translocation capacity of protein kinase C from cytosol to membrane fractions. Insulin induces a 600% activation of...

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Detalles Bibliográficos
Autor principal: Gómez, María Laura
Publicado: 1988
Materias:
epidermal growth factor
insulin
protein kinase c
thrombin
breast tumor
cell culture
human
human cell
Breast Neoplasms
Cell Division
Cell Membrane
Cytoplasm
Epidermal Growth Factor
Human
Insulin
Protein Kinase C
Support, Non-U.S. Gov't
Thrombin
Tumor Cells, Cultured
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00144827_v175_n1_p74_Gomez
http://hdl.handle.net/20.500.12110/paper_00144827_v175_n1_p74_Gomez
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_00144827_v175_n1_p74_Gomez
record_format dspace
spelling paper:paper_00144827_v175_n1_p74_Gomez2023-06-08T14:37:14Z Protein kinase C is differentially regulated by thrombin, insulin, and epidermal growth factor in human mammary tumor cells Gómez, María Laura epidermal growth factor insulin protein kinase c thrombin breast tumor cell culture human human cell Breast Neoplasms Cell Division Cell Membrane Cytoplasm Epidermal Growth Factor Human Insulin Protein Kinase C Support, Non-U.S. Gov't Thrombin Tumor Cells, Cultured The exposure of serum-deprived mammary tumor cells MCF-7 and T-47D to insulin, thrombin, and epidermal growth factor (EGF) resulted in dramatic modifications in the activity and in the translocation capacity of protein kinase C from cytosol to membrane fractions. Insulin induces a 600% activation of the enzyme after 5 h of exposure to the hormone in MCF-7 cells; thrombin either activates (200% in MCF-7) or down-regulates (in T-47D), and EGF exerts only a moderate effect. Thus, the growth factors studied modulate differentially the protein kinase C activity in human mammary tumor cells. The physiological significance of the results obtained are discussed in terms of the growth response elicited by insulin, thrombin, and EGF. © 1988. Fil:Gomez, M.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1988 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00144827_v175_n1_p74_Gomez http://hdl.handle.net/20.500.12110/paper_00144827_v175_n1_p74_Gomez
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic epidermal growth factor
insulin
protein kinase c
thrombin
breast tumor
cell culture
human
human cell
Breast Neoplasms
Cell Division
Cell Membrane
Cytoplasm
Epidermal Growth Factor
Human
Insulin
Protein Kinase C
Support, Non-U.S. Gov't
Thrombin
Tumor Cells, Cultured
spellingShingle epidermal growth factor
insulin
protein kinase c
thrombin
breast tumor
cell culture
human
human cell
Breast Neoplasms
Cell Division
Cell Membrane
Cytoplasm
Epidermal Growth Factor
Human
Insulin
Protein Kinase C
Support, Non-U.S. Gov't
Thrombin
Tumor Cells, Cultured
Gómez, María Laura
Protein kinase C is differentially regulated by thrombin, insulin, and epidermal growth factor in human mammary tumor cells
topic_facet epidermal growth factor
insulin
protein kinase c
thrombin
breast tumor
cell culture
human
human cell
Breast Neoplasms
Cell Division
Cell Membrane
Cytoplasm
Epidermal Growth Factor
Human
Insulin
Protein Kinase C
Support, Non-U.S. Gov't
Thrombin
Tumor Cells, Cultured
description The exposure of serum-deprived mammary tumor cells MCF-7 and T-47D to insulin, thrombin, and epidermal growth factor (EGF) resulted in dramatic modifications in the activity and in the translocation capacity of protein kinase C from cytosol to membrane fractions. Insulin induces a 600% activation of the enzyme after 5 h of exposure to the hormone in MCF-7 cells; thrombin either activates (200% in MCF-7) or down-regulates (in T-47D), and EGF exerts only a moderate effect. Thus, the growth factors studied modulate differentially the protein kinase C activity in human mammary tumor cells. The physiological significance of the results obtained are discussed in terms of the growth response elicited by insulin, thrombin, and EGF. © 1988.
author Gómez, María Laura
author_facet Gómez, María Laura
author_sort Gómez, María Laura
title Protein kinase C is differentially regulated by thrombin, insulin, and epidermal growth factor in human mammary tumor cells
title_short Protein kinase C is differentially regulated by thrombin, insulin, and epidermal growth factor in human mammary tumor cells
title_full Protein kinase C is differentially regulated by thrombin, insulin, and epidermal growth factor in human mammary tumor cells
title_fullStr Protein kinase C is differentially regulated by thrombin, insulin, and epidermal growth factor in human mammary tumor cells
title_full_unstemmed Protein kinase C is differentially regulated by thrombin, insulin, and epidermal growth factor in human mammary tumor cells
title_sort protein kinase c is differentially regulated by thrombin, insulin, and epidermal growth factor in human mammary tumor cells
publishDate 1988
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00144827_v175_n1_p74_Gomez
http://hdl.handle.net/20.500.12110/paper_00144827_v175_n1_p74_Gomez
work_keys_str_mv AT gomezmarialaura proteinkinasecisdifferentiallyregulatedbythrombininsulinandepidermalgrowthfactorinhumanmammarytumorcells
_version_ 1768541590120300544

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