Sex-linked differences in the vasorelaxant effects of anandamide in vascular mesenteric beds: Role of oestrogens
Anandamide (0.01 to 10 μM) caused greater concentration-dependent reductions of the contractile-induced responses to noradrenaline in female than in male mesenteric vascular beds isolated from adult Sprague-Dawley rats. Greater relaxant responses in females were also induced by the vanilloid TRPV1 r...
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2004
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142999_v493_n1-3_p151_Peroni http://hdl.handle.net/20.500.12110/paper_00142999_v493_n1-3_p151_Peroni |
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paper:paper_00142999_v493_n1-3_p151_Peroni2023-06-08T14:36:52Z Sex-linked differences in the vasorelaxant effects of anandamide in vascular mesenteric beds: Role of oestrogens (Sprague-Dawley rat) 17β-Oestradiol Anandamide Sex difference Vasorelaxation acetylcholine anandamide cannabinoid 1 receptor cannabinoid receptor antagonist capsaicin capsazepine cycloheximide estradiol estradiol benzoate estrogen nitroprusside sodium noradrenalin protein synthesis inhibitor rimonabant vanilloid receptor animal experiment animal tissue article blood vessel concentration response controlled study dose response drug effect estrogen activity exposure female in vitro study isolated organ male muscle contractility nonhuman ovariectomy priority journal rat sex difference vasodilatation Acetylcholine Animals Arachidonic Acids Argentina Capsaicin Chile Cycloheximide Dose-Response Relationship, Drug Drug Synergism Estradiol Estrogens Female Male Mesentery Muscle, Smooth, Vascular Nitroprusside Norepinephrine Ovariectomy Phenylmethylsulfonyl Fluoride Piperidines Polyunsaturated Alkamides Pyrazoles Rats Rats, Sprague-Dawley Sex Characteristics Time Factors Vasodilation Anandamide (0.01 to 10 μM) caused greater concentration-dependent reductions of the contractile-induced responses to noradrenaline in female than in male mesenteric vascular beds isolated from adult Sprague-Dawley rats. Greater relaxant responses in females were also induced by the vanilloid TRPV1 receptor agonist capsaicin (0.01 to 10 μM), whereas no sex differences were observed for the relaxations caused by either acetylcholine or sodium nitroprusside. The effect of anandamide in either sex was reduced by the vanilloid TRPV1 receptor antagonist capsazepine but not by the cannabinoid CB1 receptor antagonist N-piperidino-5-(4-chlorophenyl)-1-(2,4- dichlorophenyl)-4-methyl-3-pyrazole-carboxamide (SR141716A). In males, the anandamide-induced relaxations were potentiated by in vitro exposure during 5 min to 0.5 μM 17β-oestradiol and unmodified by the protein synthesis inhibitor cycloheximide. The vasorelaxant effects of anandamide in female rats were decreased by ovariectomy. This decrease was prevented by in vivo treatment with 17β-oestradiol-3-benzoate (450 μg/kg i.m., once a week during 3 weeks) and counteracted by in vitro exposure to oestrogen. In vivo treatment with 17β-oestradiol also potentiated anandamide-induced responses in males. In conclusion, this study shows an oestrogen-dependent sensitivity to the vanilloid TRPV1 receptor-mediated vasorelaxant effects of anandamide in the mesenteric vasculature of Sprague-Dawley rats, that could be mediated by both genomic and non-genomic mechanisms. © 2004 Elsevier B.V. All rights reserved. 2004 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142999_v493_n1-3_p151_Peroni http://hdl.handle.net/20.500.12110/paper_00142999_v493_n1-3_p151_Peroni |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
(Sprague-Dawley rat) 17β-Oestradiol Anandamide Sex difference Vasorelaxation acetylcholine anandamide cannabinoid 1 receptor cannabinoid receptor antagonist capsaicin capsazepine cycloheximide estradiol estradiol benzoate estrogen nitroprusside sodium noradrenalin protein synthesis inhibitor rimonabant vanilloid receptor animal experiment animal tissue article blood vessel concentration response controlled study dose response drug effect estrogen activity exposure female in vitro study isolated organ male muscle contractility nonhuman ovariectomy priority journal rat sex difference vasodilatation Acetylcholine Animals Arachidonic Acids Argentina Capsaicin Chile Cycloheximide Dose-Response Relationship, Drug Drug Synergism Estradiol Estrogens Female Male Mesentery Muscle, Smooth, Vascular Nitroprusside Norepinephrine Ovariectomy Phenylmethylsulfonyl Fluoride Piperidines Polyunsaturated Alkamides Pyrazoles Rats Rats, Sprague-Dawley Sex Characteristics Time Factors Vasodilation |
| spellingShingle |
(Sprague-Dawley rat) 17β-Oestradiol Anandamide Sex difference Vasorelaxation acetylcholine anandamide cannabinoid 1 receptor cannabinoid receptor antagonist capsaicin capsazepine cycloheximide estradiol estradiol benzoate estrogen nitroprusside sodium noradrenalin protein synthesis inhibitor rimonabant vanilloid receptor animal experiment animal tissue article blood vessel concentration response controlled study dose response drug effect estrogen activity exposure female in vitro study isolated organ male muscle contractility nonhuman ovariectomy priority journal rat sex difference vasodilatation Acetylcholine Animals Arachidonic Acids Argentina Capsaicin Chile Cycloheximide Dose-Response Relationship, Drug Drug Synergism Estradiol Estrogens Female Male Mesentery Muscle, Smooth, Vascular Nitroprusside Norepinephrine Ovariectomy Phenylmethylsulfonyl Fluoride Piperidines Polyunsaturated Alkamides Pyrazoles Rats Rats, Sprague-Dawley Sex Characteristics Time Factors Vasodilation Sex-linked differences in the vasorelaxant effects of anandamide in vascular mesenteric beds: Role of oestrogens |
| topic_facet |
(Sprague-Dawley rat) 17β-Oestradiol Anandamide Sex difference Vasorelaxation acetylcholine anandamide cannabinoid 1 receptor cannabinoid receptor antagonist capsaicin capsazepine cycloheximide estradiol estradiol benzoate estrogen nitroprusside sodium noradrenalin protein synthesis inhibitor rimonabant vanilloid receptor animal experiment animal tissue article blood vessel concentration response controlled study dose response drug effect estrogen activity exposure female in vitro study isolated organ male muscle contractility nonhuman ovariectomy priority journal rat sex difference vasodilatation Acetylcholine Animals Arachidonic Acids Argentina Capsaicin Chile Cycloheximide Dose-Response Relationship, Drug Drug Synergism Estradiol Estrogens Female Male Mesentery Muscle, Smooth, Vascular Nitroprusside Norepinephrine Ovariectomy Phenylmethylsulfonyl Fluoride Piperidines Polyunsaturated Alkamides Pyrazoles Rats Rats, Sprague-Dawley Sex Characteristics Time Factors Vasodilation |
| description |
Anandamide (0.01 to 10 μM) caused greater concentration-dependent reductions of the contractile-induced responses to noradrenaline in female than in male mesenteric vascular beds isolated from adult Sprague-Dawley rats. Greater relaxant responses in females were also induced by the vanilloid TRPV1 receptor agonist capsaicin (0.01 to 10 μM), whereas no sex differences were observed for the relaxations caused by either acetylcholine or sodium nitroprusside. The effect of anandamide in either sex was reduced by the vanilloid TRPV1 receptor antagonist capsazepine but not by the cannabinoid CB1 receptor antagonist N-piperidino-5-(4-chlorophenyl)-1-(2,4- dichlorophenyl)-4-methyl-3-pyrazole-carboxamide (SR141716A). In males, the anandamide-induced relaxations were potentiated by in vitro exposure during 5 min to 0.5 μM 17β-oestradiol and unmodified by the protein synthesis inhibitor cycloheximide. The vasorelaxant effects of anandamide in female rats were decreased by ovariectomy. This decrease was prevented by in vivo treatment with 17β-oestradiol-3-benzoate (450 μg/kg i.m., once a week during 3 weeks) and counteracted by in vitro exposure to oestrogen. In vivo treatment with 17β-oestradiol also potentiated anandamide-induced responses in males. In conclusion, this study shows an oestrogen-dependent sensitivity to the vanilloid TRPV1 receptor-mediated vasorelaxant effects of anandamide in the mesenteric vasculature of Sprague-Dawley rats, that could be mediated by both genomic and non-genomic mechanisms. © 2004 Elsevier B.V. All rights reserved. |
| title |
Sex-linked differences in the vasorelaxant effects of anandamide in vascular mesenteric beds: Role of oestrogens |
| title_short |
Sex-linked differences in the vasorelaxant effects of anandamide in vascular mesenteric beds: Role of oestrogens |
| title_full |
Sex-linked differences in the vasorelaxant effects of anandamide in vascular mesenteric beds: Role of oestrogens |
| title_fullStr |
Sex-linked differences in the vasorelaxant effects of anandamide in vascular mesenteric beds: Role of oestrogens |
| title_full_unstemmed |
Sex-linked differences in the vasorelaxant effects of anandamide in vascular mesenteric beds: Role of oestrogens |
| title_sort |
sex-linked differences in the vasorelaxant effects of anandamide in vascular mesenteric beds: role of oestrogens |
| publishDate |
2004 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142999_v493_n1-3_p151_Peroni http://hdl.handle.net/20.500.12110/paper_00142999_v493_n1-3_p151_Peroni |
| _version_ |
1768544485555306496 |