Activation of nitric oxide synthase through muscarinic receptors in rat parotid gland
Muscarinic receptors play an important role in secretory and vasodilator responses in rat salivary glands. Nitric oxide synthase (NOS) activity was found coupled to muscarinic receptor activation as well as to nitric oxide-mediated amylase secretion elicited by carbachol. Parotid glands presented a...
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2002
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142999_v439_n1-3_p27_Rosignoli http://hdl.handle.net/20.500.12110/paper_00142999_v439_n1-3_p27_Rosignoli |
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paper:paper_00142999_v439_n1-3_p27_Rosignoli2023-06-08T14:36:51Z Activation of nitric oxide synthase through muscarinic receptors in rat parotid gland Rosignoli, Florencia Pérez Leirós, Claudia Amylase secretion Nitric oxide (NO) signaling Nitric oxide (NO) synthase Parotid gland 4 diphenylacetoxy 1 methylpiperidine amylase carbachol methylene blue muscarinic M1 receptor muscarinic M3 receptor n(g) nitroarginine methyl ester nitric oxide nitric oxide synthase phorbol 13 acetate 12 myristate pirenzepine protein kinase C amylase release animal tissue article concentration response controlled study drug mechanism enzyme activation enzyme activity enzyme inhibition male nonhuman parotid gland priority journal rat receptor density signal transduction Amylases Animals Atropine Binding, Competitive Carbachol Cell Membrane Dose-Response Relationship, Drug Enzyme Activation Enzyme Inhibitors Male Muscarinic Antagonists NG-Nitroarginine Methyl Ester Nitric Oxide Nitric Oxide Synthase Parotid Gland Piperidines Pirenzepine Quinuclidinyl Benzilate Rats Rats, Wistar Receptors, Muscarinic Specific Pathogen-Free Organisms Muscarinic receptors play an important role in secretory and vasodilator responses in rat salivary glands. Nitric oxide synthase (NOS) activity was found coupled to muscarinic receptor activation as well as to nitric oxide-mediated amylase secretion elicited by carbachol. Parotid glands presented a predominant M3 and a minor muscarinic M1 acetylcholine receptor population, though carbachol stimulated NOS activity only through muscarinic M3 receptors as revealed in the presence of 4-diphenylacetoxy-N-methylpiperidine methiodide (4-DAMP) and pirenzepine. Amylase secretion induced by carbachol appeared to be partly mediated by nitric oxide and nitric oxide-induced signaling since N-nitro-L-arginine methyl ester (L-NAME) inhibited the effect as well as did methylene blue. A negative regulation of NOS by protein kinase C activation in the presence of a high concentration of carbachol was seen in parotid glands and this inhibition was paralleled by amylase secretion. © 2002 Elsevier Science B.V. All rights reserved. Fil:Rosignoli, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pérez Leirós, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2002 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142999_v439_n1-3_p27_Rosignoli http://hdl.handle.net/20.500.12110/paper_00142999_v439_n1-3_p27_Rosignoli |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Amylase secretion Nitric oxide (NO) signaling Nitric oxide (NO) synthase Parotid gland 4 diphenylacetoxy 1 methylpiperidine amylase carbachol methylene blue muscarinic M1 receptor muscarinic M3 receptor n(g) nitroarginine methyl ester nitric oxide nitric oxide synthase phorbol 13 acetate 12 myristate pirenzepine protein kinase C amylase release animal tissue article concentration response controlled study drug mechanism enzyme activation enzyme activity enzyme inhibition male nonhuman parotid gland priority journal rat receptor density signal transduction Amylases Animals Atropine Binding, Competitive Carbachol Cell Membrane Dose-Response Relationship, Drug Enzyme Activation Enzyme Inhibitors Male Muscarinic Antagonists NG-Nitroarginine Methyl Ester Nitric Oxide Nitric Oxide Synthase Parotid Gland Piperidines Pirenzepine Quinuclidinyl Benzilate Rats Rats, Wistar Receptors, Muscarinic Specific Pathogen-Free Organisms |
| spellingShingle |
Amylase secretion Nitric oxide (NO) signaling Nitric oxide (NO) synthase Parotid gland 4 diphenylacetoxy 1 methylpiperidine amylase carbachol methylene blue muscarinic M1 receptor muscarinic M3 receptor n(g) nitroarginine methyl ester nitric oxide nitric oxide synthase phorbol 13 acetate 12 myristate pirenzepine protein kinase C amylase release animal tissue article concentration response controlled study drug mechanism enzyme activation enzyme activity enzyme inhibition male nonhuman parotid gland priority journal rat receptor density signal transduction Amylases Animals Atropine Binding, Competitive Carbachol Cell Membrane Dose-Response Relationship, Drug Enzyme Activation Enzyme Inhibitors Male Muscarinic Antagonists NG-Nitroarginine Methyl Ester Nitric Oxide Nitric Oxide Synthase Parotid Gland Piperidines Pirenzepine Quinuclidinyl Benzilate Rats Rats, Wistar Receptors, Muscarinic Specific Pathogen-Free Organisms Rosignoli, Florencia Pérez Leirós, Claudia Activation of nitric oxide synthase through muscarinic receptors in rat parotid gland |
| topic_facet |
Amylase secretion Nitric oxide (NO) signaling Nitric oxide (NO) synthase Parotid gland 4 diphenylacetoxy 1 methylpiperidine amylase carbachol methylene blue muscarinic M1 receptor muscarinic M3 receptor n(g) nitroarginine methyl ester nitric oxide nitric oxide synthase phorbol 13 acetate 12 myristate pirenzepine protein kinase C amylase release animal tissue article concentration response controlled study drug mechanism enzyme activation enzyme activity enzyme inhibition male nonhuman parotid gland priority journal rat receptor density signal transduction Amylases Animals Atropine Binding, Competitive Carbachol Cell Membrane Dose-Response Relationship, Drug Enzyme Activation Enzyme Inhibitors Male Muscarinic Antagonists NG-Nitroarginine Methyl Ester Nitric Oxide Nitric Oxide Synthase Parotid Gland Piperidines Pirenzepine Quinuclidinyl Benzilate Rats Rats, Wistar Receptors, Muscarinic Specific Pathogen-Free Organisms |
| description |
Muscarinic receptors play an important role in secretory and vasodilator responses in rat salivary glands. Nitric oxide synthase (NOS) activity was found coupled to muscarinic receptor activation as well as to nitric oxide-mediated amylase secretion elicited by carbachol. Parotid glands presented a predominant M3 and a minor muscarinic M1 acetylcholine receptor population, though carbachol stimulated NOS activity only through muscarinic M3 receptors as revealed in the presence of 4-diphenylacetoxy-N-methylpiperidine methiodide (4-DAMP) and pirenzepine. Amylase secretion induced by carbachol appeared to be partly mediated by nitric oxide and nitric oxide-induced signaling since N-nitro-L-arginine methyl ester (L-NAME) inhibited the effect as well as did methylene blue. A negative regulation of NOS by protein kinase C activation in the presence of a high concentration of carbachol was seen in parotid glands and this inhibition was paralleled by amylase secretion. © 2002 Elsevier Science B.V. All rights reserved. |
| author |
Rosignoli, Florencia Pérez Leirós, Claudia |
| author_facet |
Rosignoli, Florencia Pérez Leirós, Claudia |
| author_sort |
Rosignoli, Florencia |
| title |
Activation of nitric oxide synthase through muscarinic receptors in rat parotid gland |
| title_short |
Activation of nitric oxide synthase through muscarinic receptors in rat parotid gland |
| title_full |
Activation of nitric oxide synthase through muscarinic receptors in rat parotid gland |
| title_fullStr |
Activation of nitric oxide synthase through muscarinic receptors in rat parotid gland |
| title_full_unstemmed |
Activation of nitric oxide synthase through muscarinic receptors in rat parotid gland |
| title_sort |
activation of nitric oxide synthase through muscarinic receptors in rat parotid gland |
| publishDate |
2002 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142999_v439_n1-3_p27_Rosignoli http://hdl.handle.net/20.500.12110/paper_00142999_v439_n1-3_p27_Rosignoli |
| work_keys_str_mv |
AT rosignoliflorencia activationofnitricoxidesynthasethroughmuscarinicreceptorsinratparotidgland AT perezleirosclaudia activationofnitricoxidesynthasethroughmuscarinicreceptorsinratparotidgland |
| _version_ |
1768545170689622016 |