Synthesis of Cellulose Precursors: The Involvement of Lipid‐Linked Sugars

Particulate preparations from the Chlorophyta Prototheca zopfii catalyze the incorporation of [14C]glucose from UDP‐[14C]glucose into lipids. These lipids have been characterized as lipid‐P‐glucose, lipid‐PP‐glucose, and lipid‐PP‐oligosaccharides. The lipid‐linked oligosaccharides were a mixture ran...

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Detalles Bibliográficos
Publicado:	1978
Materias:	cellulose radioisotope animal experiment cell wall in vitro study Algae, Green Cellulose Dolichol Glycolipids Guanosine Diphosphate Sugars Oligosaccharides Polyisoprenyl Phosphates Temperature Uridine Diphosphate Uridine Diphosphate Glucose Uridine Monophosphate
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142956_v84_n2_p561_HOPP http://hdl.handle.net/20.500.12110/paper_00142956_v84_n2_p561_HOPP
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00142956_v84_n2_p561_HOPP
record_format	dspace
spelling	paper:paper_00142956_v84_n2_p561_HOPP2023-06-08T14:36:46Z Synthesis of Cellulose Precursors: The Involvement of Lipid‐Linked Sugars cellulose radioisotope animal experiment cell wall in vitro study Algae, Green Cellulose Dolichol Glycolipids Guanosine Diphosphate Sugars Oligosaccharides Polyisoprenyl Phosphates Temperature Uridine Diphosphate Uridine Diphosphate Glucose Uridine Monophosphate Particulate preparations from the Chlorophyta Prototheca zopfii catalyze the incorporation of [14C]glucose from UDP‐[14C]glucose into lipids. These lipids have been characterized as lipid‐P‐glucose, lipid‐PP‐glucose, and lipid‐PP‐oligosaccharides. The lipid‐linked oligosaccharides were a mixture ranging from a disaccharide to approximately a decasaccharide. Cellulase digestion and periodate oxidation showed that the oligosaccharides seem to be built of β‐1,4‐linked glucoses. The lipid moiety had the properties of dolichol. The glucolipids described appeared as precursors of a water‐soluble polymer. Treatments of this polymer with hydrolytic enzymes and periodate oxidation indicate that it could be a glycoprotein containing β‐1,4‐linked glucoses. When GDP‐glucose was added to the incubation mixture, the 14C‐labelled soluble polymer became insoluble in hot alkali. This insoluble polymer had the properties expected for cellulose. A scheme is proposed with the reactions involved in the initiation of cellulose biosynthesis. Copyright © 1978, Wiley Blackwell. All rights reserved 1978 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142956_v84_n2_p561_HOPP http://hdl.handle.net/20.500.12110/paper_00142956_v84_n2_p561_HOPP
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	cellulose radioisotope animal experiment cell wall in vitro study Algae, Green Cellulose Dolichol Glycolipids Guanosine Diphosphate Sugars Oligosaccharides Polyisoprenyl Phosphates Temperature Uridine Diphosphate Uridine Diphosphate Glucose Uridine Monophosphate
spellingShingle	cellulose radioisotope animal experiment cell wall in vitro study Algae, Green Cellulose Dolichol Glycolipids Guanosine Diphosphate Sugars Oligosaccharides Polyisoprenyl Phosphates Temperature Uridine Diphosphate Uridine Diphosphate Glucose Uridine Monophosphate Synthesis of Cellulose Precursors: The Involvement of Lipid‐Linked Sugars
topic_facet	cellulose radioisotope animal experiment cell wall in vitro study Algae, Green Cellulose Dolichol Glycolipids Guanosine Diphosphate Sugars Oligosaccharides Polyisoprenyl Phosphates Temperature Uridine Diphosphate Uridine Diphosphate Glucose Uridine Monophosphate
description	Particulate preparations from the Chlorophyta Prototheca zopfii catalyze the incorporation of [14C]glucose from UDP‐[14C]glucose into lipids. These lipids have been characterized as lipid‐P‐glucose, lipid‐PP‐glucose, and lipid‐PP‐oligosaccharides. The lipid‐linked oligosaccharides were a mixture ranging from a disaccharide to approximately a decasaccharide. Cellulase digestion and periodate oxidation showed that the oligosaccharides seem to be built of β‐1,4‐linked glucoses. The lipid moiety had the properties of dolichol. The glucolipids described appeared as precursors of a water‐soluble polymer. Treatments of this polymer with hydrolytic enzymes and periodate oxidation indicate that it could be a glycoprotein containing β‐1,4‐linked glucoses. When GDP‐glucose was added to the incubation mixture, the 14C‐labelled soluble polymer became insoluble in hot alkali. This insoluble polymer had the properties expected for cellulose. A scheme is proposed with the reactions involved in the initiation of cellulose biosynthesis. Copyright © 1978, Wiley Blackwell. All rights reserved
title	Synthesis of Cellulose Precursors: The Involvement of Lipid‐Linked Sugars
title_short	Synthesis of Cellulose Precursors: The Involvement of Lipid‐Linked Sugars
title_full	Synthesis of Cellulose Precursors: The Involvement of Lipid‐Linked Sugars
title_fullStr	Synthesis of Cellulose Precursors: The Involvement of Lipid‐Linked Sugars
title_full_unstemmed	Synthesis of Cellulose Precursors: The Involvement of Lipid‐Linked Sugars
title_sort	synthesis of cellulose precursors: the involvement of lipid‐linked sugars
publishDate	1978
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142956_v84_n2_p561_HOPP http://hdl.handle.net/20.500.12110/paper_00142956_v84_n2_p561_HOPP
_version_	1768543828602519552

Synthesis of Cellulose Precursors: The Involvement of Lipid‐Linked Sugars

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