Porphyrin Biosynthesis: 5. Biosynthesis of Protoporphyrin IX in Harderian Glands
The enzymic system that catalyzes the total synthesis of protoporphyrin IX has been found in the Harderian gland of the rat. The presence of the enzyme δ‐aminolevulinic acid synthetase was demonstrated in mitochondria by the formation of [14C]δ‐aminolevulinic acid. On incubation of homogenates of th...
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1968
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142956_v6_n1_p80_Tomio http://hdl.handle.net/20.500.12110/paper_00142956_v6_n1_p80_Tomio |
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paper:paper_00142956_v6_n1_p80_Tomio2023-06-08T14:36:44Z Porphyrin Biosynthesis: 5. Biosynthesis of Protoporphyrin IX in Harderian Glands Tomio, Josefina María Grinstein, Moisés amino acid carbon coenzyme A glycine levulinic acid ligase porphyrin succinic acid derivative animal article biosynthesis enzymology lacrimal apparatus male metabolism mitochondrion nictitating membrane rat Amino Acids Animal Carbon Isotopes Coenzyme A Glycine Lacrimal Apparatus Levulinic Acids Ligases Male Mitochondria Nictitating Membrane Porphyrins Rats Succinates The enzymic system that catalyzes the total synthesis of protoporphyrin IX has been found in the Harderian gland of the rat. The presence of the enzyme δ‐aminolevulinic acid synthetase was demonstrated in mitochondria by the formation of [14C]δ‐aminolevulinic acid. On incubation of homogenates of the Harderian glands with δ‐aminolevulinic acid and porphobilinogen, varying conditions such as temperature, time, aero‐or anaerobiosis and cofactor and substrate concentration, porphyrins with a carboxyl content ranging from 8–2 were formed. Thus, the presence of δ‐aminolevulinate dehydratase, porphobilinogenase, decarboxylase and coproporphyrinogenase systems were demonstrated. Copyright © 1968, Wiley Blackwell. All rights reserved Fil:Tomio, J.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Grinstein, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1968 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142956_v6_n1_p80_Tomio http://hdl.handle.net/20.500.12110/paper_00142956_v6_n1_p80_Tomio |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
amino acid carbon coenzyme A glycine levulinic acid ligase porphyrin succinic acid derivative animal article biosynthesis enzymology lacrimal apparatus male metabolism mitochondrion nictitating membrane rat Amino Acids Animal Carbon Isotopes Coenzyme A Glycine Lacrimal Apparatus Levulinic Acids Ligases Male Mitochondria Nictitating Membrane Porphyrins Rats Succinates |
| spellingShingle |
amino acid carbon coenzyme A glycine levulinic acid ligase porphyrin succinic acid derivative animal article biosynthesis enzymology lacrimal apparatus male metabolism mitochondrion nictitating membrane rat Amino Acids Animal Carbon Isotopes Coenzyme A Glycine Lacrimal Apparatus Levulinic Acids Ligases Male Mitochondria Nictitating Membrane Porphyrins Rats Succinates Tomio, Josefina María Grinstein, Moisés Porphyrin Biosynthesis: 5. Biosynthesis of Protoporphyrin IX in Harderian Glands |
| topic_facet |
amino acid carbon coenzyme A glycine levulinic acid ligase porphyrin succinic acid derivative animal article biosynthesis enzymology lacrimal apparatus male metabolism mitochondrion nictitating membrane rat Amino Acids Animal Carbon Isotopes Coenzyme A Glycine Lacrimal Apparatus Levulinic Acids Ligases Male Mitochondria Nictitating Membrane Porphyrins Rats Succinates |
| description |
The enzymic system that catalyzes the total synthesis of protoporphyrin IX has been found in the Harderian gland of the rat. The presence of the enzyme δ‐aminolevulinic acid synthetase was demonstrated in mitochondria by the formation of [14C]δ‐aminolevulinic acid. On incubation of homogenates of the Harderian glands with δ‐aminolevulinic acid and porphobilinogen, varying conditions such as temperature, time, aero‐or anaerobiosis and cofactor and substrate concentration, porphyrins with a carboxyl content ranging from 8–2 were formed. Thus, the presence of δ‐aminolevulinate dehydratase, porphobilinogenase, decarboxylase and coproporphyrinogenase systems were demonstrated. Copyright © 1968, Wiley Blackwell. All rights reserved |
| author |
Tomio, Josefina María Grinstein, Moisés |
| author_facet |
Tomio, Josefina María Grinstein, Moisés |
| author_sort |
Tomio, Josefina María |
| title |
Porphyrin Biosynthesis: 5. Biosynthesis of Protoporphyrin IX in Harderian Glands |
| title_short |
Porphyrin Biosynthesis: 5. Biosynthesis of Protoporphyrin IX in Harderian Glands |
| title_full |
Porphyrin Biosynthesis: 5. Biosynthesis of Protoporphyrin IX in Harderian Glands |
| title_fullStr |
Porphyrin Biosynthesis: 5. Biosynthesis of Protoporphyrin IX in Harderian Glands |
| title_full_unstemmed |
Porphyrin Biosynthesis: 5. Biosynthesis of Protoporphyrin IX in Harderian Glands |
| title_sort |
porphyrin biosynthesis: 5. biosynthesis of protoporphyrin ix in harderian glands |
| publishDate |
1968 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142956_v6_n1_p80_Tomio http://hdl.handle.net/20.500.12110/paper_00142956_v6_n1_p80_Tomio |
| work_keys_str_mv |
AT tomiojosefinamaria porphyrinbiosynthesis5biosynthesisofprotoporphyrinixinharderianglands AT grinsteinmoises porphyrinbiosynthesis5biosynthesisofprotoporphyrinixinharderianglands |
| _version_ |
1768542919781777408 |