Reconstitution of a light‐stimulated adenylate cyclase from retina and Neurospora crassa preparations: Characterization of the heterologous systems using normal and degenerative retinas

Adenylate cyclase catalytic subunits from Neurospora crassa membranes may interact with regulatory factors from membranes of bovine retinal rod outer segments (pretreated with N‐ethylmaleimide), reconstituting a heterologous system which, in the presence of light, is catalytically active in assay mi...

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Detalles Bibliográficos
Autores principales:	Muschietti, Jorge P., Bianchini, Graciela Mabel, Martinetto, Horacio E., Carricarte, Valentina, Torres, Héctor Norberto, Flawiá, Mirtha María
Publicado:	1989
Materias:	adenylate cyclase radioisotope animal cell cattle fungus light membrane neurospora crassa nonhuman priority journal retina Adenosine Diphosphate Ribose Adenylate Cyclase Animal Cattle Cyclic GMP Light Mice Mice, Inbred C57BL Neurospora Neurospora crassa Retina
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142956_v185_n1_p205_Muschietti http://hdl.handle.net/20.500.12110/paper_00142956_v185_n1_p205_Muschietti
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00142956_v185_n1_p205_Muschietti
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spelling	paper:paper_00142956_v185_n1_p205_Muschietti2023-06-08T14:36:41Z Reconstitution of a light‐stimulated adenylate cyclase from retina and Neurospora crassa preparations: Characterization of the heterologous systems using normal and degenerative retinas Muschietti, Jorge P. Bianchini, Graciela Mabel Martinetto, Horacio E. Carricarte, Valentina Torres, Héctor Norberto Flawiá, Mirtha María adenylate cyclase radioisotope animal cell cattle fungus light membrane neurospora crassa nonhuman priority journal retina Adenosine Diphosphate Ribose Adenylate Cyclase Animal Cattle Cyclic GMP Light Mice Mice, Inbred C57BL Neurospora Neurospora crassa Retina Adenylate cyclase catalytic subunits from Neurospora crassa membranes may interact with regulatory factors from membranes of bovine retinal rod outer segments (pretreated with N‐ethylmaleimide), reconstituting a heterologous system which, in the presence of light, is catalytically active in assay mixtures containing MgATP. Maximal activation was observed at 550 nm. Transducin‐depleted retinal membranes were not capable of reconstituting the heterologous light‐stimulated adenylate cyclase system. Addition of a transducin preparation to depleted membranes restored the reconstitution capacity of these membranes. A similar heterologous adenylate cyclase system was reconstituted with Neurospora and mouse retinal whole membranes (pretreated with N‐ethylmaleimide). Membranes from mice suffering photoreceptor degeneration (rd homozygotes) did not reconstitute an heterologous adenylate cyclase system. Copyright © 1989, Wiley Blackwell. All rights reserved Fil:Muschietti, J.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bianchini, G.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Martinetto, H.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Carricarte, V.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Torres, H.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Flawia, M.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1989 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142956_v185_n1_p205_Muschietti http://hdl.handle.net/20.500.12110/paper_00142956_v185_n1_p205_Muschietti
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	adenylate cyclase radioisotope animal cell cattle fungus light membrane neurospora crassa nonhuman priority journal retina Adenosine Diphosphate Ribose Adenylate Cyclase Animal Cattle Cyclic GMP Light Mice Mice, Inbred C57BL Neurospora Neurospora crassa Retina
spellingShingle	adenylate cyclase radioisotope animal cell cattle fungus light membrane neurospora crassa nonhuman priority journal retina Adenosine Diphosphate Ribose Adenylate Cyclase Animal Cattle Cyclic GMP Light Mice Mice, Inbred C57BL Neurospora Neurospora crassa Retina Muschietti, Jorge P. Bianchini, Graciela Mabel Martinetto, Horacio E. Carricarte, Valentina Torres, Héctor Norberto Flawiá, Mirtha María Reconstitution of a light‐stimulated adenylate cyclase from retina and Neurospora crassa preparations: Characterization of the heterologous systems using normal and degenerative retinas
topic_facet	adenylate cyclase radioisotope animal cell cattle fungus light membrane neurospora crassa nonhuman priority journal retina Adenosine Diphosphate Ribose Adenylate Cyclase Animal Cattle Cyclic GMP Light Mice Mice, Inbred C57BL Neurospora Neurospora crassa Retina
description	Adenylate cyclase catalytic subunits from Neurospora crassa membranes may interact with regulatory factors from membranes of bovine retinal rod outer segments (pretreated with N‐ethylmaleimide), reconstituting a heterologous system which, in the presence of light, is catalytically active in assay mixtures containing MgATP. Maximal activation was observed at 550 nm. Transducin‐depleted retinal membranes were not capable of reconstituting the heterologous light‐stimulated adenylate cyclase system. Addition of a transducin preparation to depleted membranes restored the reconstitution capacity of these membranes. A similar heterologous adenylate cyclase system was reconstituted with Neurospora and mouse retinal whole membranes (pretreated with N‐ethylmaleimide). Membranes from mice suffering photoreceptor degeneration (rd homozygotes) did not reconstitute an heterologous adenylate cyclase system. Copyright © 1989, Wiley Blackwell. All rights reserved
author	Muschietti, Jorge P. Bianchini, Graciela Mabel Martinetto, Horacio E. Carricarte, Valentina Torres, Héctor Norberto Flawiá, Mirtha María
author_facet	Muschietti, Jorge P. Bianchini, Graciela Mabel Martinetto, Horacio E. Carricarte, Valentina Torres, Héctor Norberto Flawiá, Mirtha María
author_sort	Muschietti, Jorge P.
title	Reconstitution of a light‐stimulated adenylate cyclase from retina and Neurospora crassa preparations: Characterization of the heterologous systems using normal and degenerative retinas
title_short	Reconstitution of a light‐stimulated adenylate cyclase from retina and Neurospora crassa preparations: Characterization of the heterologous systems using normal and degenerative retinas
title_full	Reconstitution of a light‐stimulated adenylate cyclase from retina and Neurospora crassa preparations: Characterization of the heterologous systems using normal and degenerative retinas
title_fullStr	Reconstitution of a light‐stimulated adenylate cyclase from retina and Neurospora crassa preparations: Characterization of the heterologous systems using normal and degenerative retinas
title_full_unstemmed	Reconstitution of a light‐stimulated adenylate cyclase from retina and Neurospora crassa preparations: Characterization of the heterologous systems using normal and degenerative retinas
title_sort	reconstitution of a light‐stimulated adenylate cyclase from retina and neurospora crassa preparations: characterization of the heterologous systems using normal and degenerative retinas
publishDate	1989
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142956_v185_n1_p205_Muschietti http://hdl.handle.net/20.500.12110/paper_00142956_v185_n1_p205_Muschietti
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_version_	1768546237355655168

Reconstitution of a light‐stimulated adenylate cyclase from retina and Neurospora crassa preparations: Characterization of the heterologous systems using normal and degenerative retinas

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