Requirement of DNA topoisomerases for in vitro chromatin assembly by 3T6 mouse cell extracts
Nuclear extracts of 3T6 mouse cells were able to assemble in vitro minichromosomes which displayed a 150‐bp periodicity. Activities of both DNA topoisomerases I and II were detected in these extracts. When a supercoiled pUC DNA was added, it was first relaxed in less than 3 min, then slowly supercoi...
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1989
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142956_v181_n2_p531_CACERES http://hdl.handle.net/20.500.12110/paper_00142956_v181_n2_p531_CACERES |
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paper:paper_00142956_v181_n2_p531_CACERES2023-06-08T14:36:40Z Requirement of DNA topoisomerases for in vitro chromatin assembly by 3T6 mouse cell extracts Cáceres, Javier Fernando Glikin, Gerardo Claudio DNA DNA topoisomerase DNA topoisomerase (ATP hydrolysing) animal article cell culture chromatin DNA supercoiling isolation and purification kinetics metabolism mouse Animal Cells, Cultured Chromatin DNA DNA Topoisomerases, Type I DNA Topoisomerases, Type II DNA, Superhelical Kinetics Mice Support, Non-U.S. Gov't Nuclear extracts of 3T6 mouse cells were able to assemble in vitro minichromosomes which displayed a 150‐bp periodicity. Activities of both DNA topoisomerases I and II were detected in these extracts. When a supercoiled pUC DNA was added, it was first relaxed in less than 3 min, then slowly supercoiled again in 1–4 h. Both reactions occurred either in the absence or the presence of added Mg2+ and/or ATP, they were not blocked by DNA topoisomerase II inhibitors and they were inhibited by an antiserum against DNA topoisomerase I and by camptothecin. These findings led us to propose that, under our in vitro assay conditions, chromatin assembly is mainly carried out by a DNA topoisomerase I. Copyright © 1989, Wiley Blackwell. All rights reserved Fil:CÁCERES, J.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:GLIKIN, G.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1989 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142956_v181_n2_p531_CACERES http://hdl.handle.net/20.500.12110/paper_00142956_v181_n2_p531_CACERES |
| institution |
Universidad de Buenos Aires |
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I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
DNA DNA topoisomerase DNA topoisomerase (ATP hydrolysing) animal article cell culture chromatin DNA supercoiling isolation and purification kinetics metabolism mouse Animal Cells, Cultured Chromatin DNA DNA Topoisomerases, Type I DNA Topoisomerases, Type II DNA, Superhelical Kinetics Mice Support, Non-U.S. Gov't |
| spellingShingle |
DNA DNA topoisomerase DNA topoisomerase (ATP hydrolysing) animal article cell culture chromatin DNA supercoiling isolation and purification kinetics metabolism mouse Animal Cells, Cultured Chromatin DNA DNA Topoisomerases, Type I DNA Topoisomerases, Type II DNA, Superhelical Kinetics Mice Support, Non-U.S. Gov't Cáceres, Javier Fernando Glikin, Gerardo Claudio Requirement of DNA topoisomerases for in vitro chromatin assembly by 3T6 mouse cell extracts |
| topic_facet |
DNA DNA topoisomerase DNA topoisomerase (ATP hydrolysing) animal article cell culture chromatin DNA supercoiling isolation and purification kinetics metabolism mouse Animal Cells, Cultured Chromatin DNA DNA Topoisomerases, Type I DNA Topoisomerases, Type II DNA, Superhelical Kinetics Mice Support, Non-U.S. Gov't |
| description |
Nuclear extracts of 3T6 mouse cells were able to assemble in vitro minichromosomes which displayed a 150‐bp periodicity. Activities of both DNA topoisomerases I and II were detected in these extracts. When a supercoiled pUC DNA was added, it was first relaxed in less than 3 min, then slowly supercoiled again in 1–4 h. Both reactions occurred either in the absence or the presence of added Mg2+ and/or ATP, they were not blocked by DNA topoisomerase II inhibitors and they were inhibited by an antiserum against DNA topoisomerase I and by camptothecin. These findings led us to propose that, under our in vitro assay conditions, chromatin assembly is mainly carried out by a DNA topoisomerase I. Copyright © 1989, Wiley Blackwell. All rights reserved |
| author |
Cáceres, Javier Fernando Glikin, Gerardo Claudio |
| author_facet |
Cáceres, Javier Fernando Glikin, Gerardo Claudio |
| author_sort |
Cáceres, Javier Fernando |
| title |
Requirement of DNA topoisomerases for in vitro chromatin assembly by 3T6 mouse cell extracts |
| title_short |
Requirement of DNA topoisomerases for in vitro chromatin assembly by 3T6 mouse cell extracts |
| title_full |
Requirement of DNA topoisomerases for in vitro chromatin assembly by 3T6 mouse cell extracts |
| title_fullStr |
Requirement of DNA topoisomerases for in vitro chromatin assembly by 3T6 mouse cell extracts |
| title_full_unstemmed |
Requirement of DNA topoisomerases for in vitro chromatin assembly by 3T6 mouse cell extracts |
| title_sort |
requirement of dna topoisomerases for in vitro chromatin assembly by 3t6 mouse cell extracts |
| publishDate |
1989 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142956_v181_n2_p531_CACERES http://hdl.handle.net/20.500.12110/paper_00142956_v181_n2_p531_CACERES |
| work_keys_str_mv |
AT caceresjavierfernando requirementofdnatopoisomerasesforinvitrochromatinassemblyby3t6mousecellextracts AT glikingerardoclaudio requirementofdnatopoisomerasesforinvitrochromatinassemblyby3t6mousecellextracts |
| _version_ |
1768544804854038528 |