Inhibitory Effect of 1‐β‐d‐Arabinofuranosylcytosine on the Synthesis of Phosphatidyl‐dCMP

The synthesis of phosphatidyl‐dCMP in mouse thymocytes is inhibited by the antineoplastic agent 1‐β‐d‐arabinofuranosyl cytosine (AraCyt)50% inhibition (ID50) being reached at an AraCyt concentration of 0.18 mM. IN the same cells, ID50 for DNA synthesis is 0.03 mM. This inhibition is probably mediate...

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Autor principal:	Iujvidin, Sonia
Publicado:	1983
Materias:	cytarabine deoxyribonuclease radioisotope cancer chemotherapy drug inhibition in vitro study mouse nonhuman phosphatidyldeoxycytidine phosphate therapy thymocyte thymus Animal Cytarabine Deoxycytidine Monophosphate Deoxycytosine Nucleotides In Vitro Mice Mice, Inbred BALB C Support, Non-U.S. Gov't Thymus Gland
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142956_v130_n2_p293_IUJVIDIN http://hdl.handle.net/20.500.12110/paper_00142956_v130_n2_p293_IUJVIDIN
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00142956_v130_n2_p293_IUJVIDIN
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spelling	paper:paper_00142956_v130_n2_p293_IUJVIDIN2023-06-08T14:36:37Z Inhibitory Effect of 1‐β‐d‐Arabinofuranosylcytosine on the Synthesis of Phosphatidyl‐dCMP Iujvidin, Sonia cytarabine deoxyribonuclease radioisotope cancer chemotherapy drug inhibition in vitro study mouse nonhuman phosphatidyldeoxycytidine phosphate therapy thymocyte thymus Animal Cytarabine Deoxycytidine Monophosphate Deoxycytosine Nucleotides In Vitro Mice Mice, Inbred BALB C Support, Non-U.S. Gov't Thymus Gland The synthesis of phosphatidyl‐dCMP in mouse thymocytes is inhibited by the antineoplastic agent 1‐β‐d‐arabinofuranosyl cytosine (AraCyt)50% inhibition (ID50) being reached at an AraCyt concentration of 0.18 mM. IN the same cells, ID50 for DNA synthesis is 0.03 mM. This inhibition is probably mediated by the phosphorylated derivative of AraCyt (aCTP) since the synthesis of phosphatidyl‐dCMP from dCTP using permeabilized thymocytes is inhibited by a aCTP (ID50= 0.11 mM). The incorporatioin of [3H]AraCyt inbto the organic phase could also be detected, suggesting that this drug may act as a substrate for the enzyme that catalyzes the transfer of dCTP into phosphatidic acid. Copyright © 1983, Wiley Blackwell. All rights reserved Fil:IUJVIDIN, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1983 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142956_v130_n2_p293_IUJVIDIN http://hdl.handle.net/20.500.12110/paper_00142956_v130_n2_p293_IUJVIDIN
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	cytarabine deoxyribonuclease radioisotope cancer chemotherapy drug inhibition in vitro study mouse nonhuman phosphatidyldeoxycytidine phosphate therapy thymocyte thymus Animal Cytarabine Deoxycytidine Monophosphate Deoxycytosine Nucleotides In Vitro Mice Mice, Inbred BALB C Support, Non-U.S. Gov't Thymus Gland
spellingShingle	cytarabine deoxyribonuclease radioisotope cancer chemotherapy drug inhibition in vitro study mouse nonhuman phosphatidyldeoxycytidine phosphate therapy thymocyte thymus Animal Cytarabine Deoxycytidine Monophosphate Deoxycytosine Nucleotides In Vitro Mice Mice, Inbred BALB C Support, Non-U.S. Gov't Thymus Gland Iujvidin, Sonia Inhibitory Effect of 1‐β‐d‐Arabinofuranosylcytosine on the Synthesis of Phosphatidyl‐dCMP
topic_facet	cytarabine deoxyribonuclease radioisotope cancer chemotherapy drug inhibition in vitro study mouse nonhuman phosphatidyldeoxycytidine phosphate therapy thymocyte thymus Animal Cytarabine Deoxycytidine Monophosphate Deoxycytosine Nucleotides In Vitro Mice Mice, Inbred BALB C Support, Non-U.S. Gov't Thymus Gland
description	The synthesis of phosphatidyl‐dCMP in mouse thymocytes is inhibited by the antineoplastic agent 1‐β‐d‐arabinofuranosyl cytosine (AraCyt)50% inhibition (ID50) being reached at an AraCyt concentration of 0.18 mM. IN the same cells, ID50 for DNA synthesis is 0.03 mM. This inhibition is probably mediated by the phosphorylated derivative of AraCyt (aCTP) since the synthesis of phosphatidyl‐dCMP from dCTP using permeabilized thymocytes is inhibited by a aCTP (ID50= 0.11 mM). The incorporatioin of [3H]AraCyt inbto the organic phase could also be detected, suggesting that this drug may act as a substrate for the enzyme that catalyzes the transfer of dCTP into phosphatidic acid. Copyright © 1983, Wiley Blackwell. All rights reserved
author	Iujvidin, Sonia
author_facet	Iujvidin, Sonia
author_sort	Iujvidin, Sonia
title	Inhibitory Effect of 1‐β‐d‐Arabinofuranosylcytosine on the Synthesis of Phosphatidyl‐dCMP
title_short	Inhibitory Effect of 1‐β‐d‐Arabinofuranosylcytosine on the Synthesis of Phosphatidyl‐dCMP
title_full	Inhibitory Effect of 1‐β‐d‐Arabinofuranosylcytosine on the Synthesis of Phosphatidyl‐dCMP
title_fullStr	Inhibitory Effect of 1‐β‐d‐Arabinofuranosylcytosine on the Synthesis of Phosphatidyl‐dCMP
title_full_unstemmed	Inhibitory Effect of 1‐β‐d‐Arabinofuranosylcytosine on the Synthesis of Phosphatidyl‐dCMP
title_sort	inhibitory effect of 1‐β‐d‐arabinofuranosylcytosine on the synthesis of phosphatidyl‐dcmp
publishDate	1983
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142956_v130_n2_p293_IUJVIDIN http://hdl.handle.net/20.500.12110/paper_00142956_v130_n2_p293_IUJVIDIN
work_keys_str_mv	AT iujvidinsonia inhibitoryeffectof1bdarabinofuranosylcytosineonthesynthesisofphosphatidyldcmp
_version_	1768546611605012480

Inhibitory Effect of 1‐β‐d‐Arabinofuranosylcytosine on the Synthesis of Phosphatidyl‐dCMP

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