Studies on Biosynthesis, Assembly and Expression of Human Major Transplantation Antigens

Biosynthesis and regulation of expression of transplantation antigens as detected by a monoclonal antibody to HLA‐A,B,C antigens (human leucocytic antigen) and a polyclonal antiserum to β2‐micro‐globulin have been investigated using radioactive amino acids and sugars to label human lymphoid cells. W...

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Publicado:	1980
Materias:	histocompatibility antigen HLA antigen monoclonal antibody heredity human cell lymphatic system normal human beta 2-Microglobulin Beta-Globulins Cell Line Electrophoresis, Polyacrylamide Gel Epitopes Histocompatibility Antigens Human Lymphoid Tissue Macromolecular Systems Translation, Genetic Tunicamycin
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142956_v103_n1_p197_ALGRANATI http://hdl.handle.net/20.500.12110/paper_00142956_v103_n1_p197_ALGRANATI
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00142956_v103_n1_p197_ALGRANATI
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spelling	paper:paper_00142956_v103_n1_p197_ALGRANATI2023-06-08T14:36:35Z Studies on Biosynthesis, Assembly and Expression of Human Major Transplantation Antigens histocompatibility antigen HLA antigen monoclonal antibody heredity human cell lymphatic system normal human beta 2-Microglobulin Beta-Globulins Cell Line Electrophoresis, Polyacrylamide Gel Epitopes Histocompatibility Antigens Human Lymphoid Tissue Macromolecular Systems Translation, Genetic Tunicamycin Biosynthesis and regulation of expression of transplantation antigens as detected by a monoclonal antibody to HLA‐A,B,C antigens (human leucocytic antigen) and a polyclonal antiserum to β2‐micro‐globulin have been investigated using radioactive amino acids and sugars to label human lymphoid cells. We found unbalanced synthesis of HLA heavy chains and β2‐microglobulin, the latter being in excess and secreted to the extracellular medium. In DAUDI cells, which are defective in β2‐microglobulin, no HLA‐A,B,C could be detected intracellularly even in the presence of added β2‐microglobulin. Treatment of BRI‐8 cells with tunicamycin, an antibiotic which inhibits glycosylation of polypeptides, almost had no effect on the levels of β2‐microglobulin, while it markedly decreased that of HLA heavy chains, both on the cell surface and intracellularly. Glycosylation of the HLA heavy chains appeared to be an essential requirement for the normal expression of HLA‐A,B,C antigens. The translation in vitro in a messenger‐dependent reticulocyte system with total polysomes obtained from BRI‐8 cells showed that β2‐microglobulin was synthesized as a precursor. This larger polypeptide was converted into mature β2‐microglobulin when protein synthesis was performed with microsomes instead of polysomes. Copyright © 1980, Wiley Blackwell. All rights reserved 1980 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142956_v103_n1_p197_ALGRANATI http://hdl.handle.net/20.500.12110/paper_00142956_v103_n1_p197_ALGRANATI
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	histocompatibility antigen HLA antigen monoclonal antibody heredity human cell lymphatic system normal human beta 2-Microglobulin Beta-Globulins Cell Line Electrophoresis, Polyacrylamide Gel Epitopes Histocompatibility Antigens Human Lymphoid Tissue Macromolecular Systems Translation, Genetic Tunicamycin
spellingShingle	histocompatibility antigen HLA antigen monoclonal antibody heredity human cell lymphatic system normal human beta 2-Microglobulin Beta-Globulins Cell Line Electrophoresis, Polyacrylamide Gel Epitopes Histocompatibility Antigens Human Lymphoid Tissue Macromolecular Systems Translation, Genetic Tunicamycin Studies on Biosynthesis, Assembly and Expression of Human Major Transplantation Antigens
topic_facet	histocompatibility antigen HLA antigen monoclonal antibody heredity human cell lymphatic system normal human beta 2-Microglobulin Beta-Globulins Cell Line Electrophoresis, Polyacrylamide Gel Epitopes Histocompatibility Antigens Human Lymphoid Tissue Macromolecular Systems Translation, Genetic Tunicamycin
description	Biosynthesis and regulation of expression of transplantation antigens as detected by a monoclonal antibody to HLA‐A,B,C antigens (human leucocytic antigen) and a polyclonal antiserum to β2‐micro‐globulin have been investigated using radioactive amino acids and sugars to label human lymphoid cells. We found unbalanced synthesis of HLA heavy chains and β2‐microglobulin, the latter being in excess and secreted to the extracellular medium. In DAUDI cells, which are defective in β2‐microglobulin, no HLA‐A,B,C could be detected intracellularly even in the presence of added β2‐microglobulin. Treatment of BRI‐8 cells with tunicamycin, an antibiotic which inhibits glycosylation of polypeptides, almost had no effect on the levels of β2‐microglobulin, while it markedly decreased that of HLA heavy chains, both on the cell surface and intracellularly. Glycosylation of the HLA heavy chains appeared to be an essential requirement for the normal expression of HLA‐A,B,C antigens. The translation in vitro in a messenger‐dependent reticulocyte system with total polysomes obtained from BRI‐8 cells showed that β2‐microglobulin was synthesized as a precursor. This larger polypeptide was converted into mature β2‐microglobulin when protein synthesis was performed with microsomes instead of polysomes. Copyright © 1980, Wiley Blackwell. All rights reserved
title	Studies on Biosynthesis, Assembly and Expression of Human Major Transplantation Antigens
title_short	Studies on Biosynthesis, Assembly and Expression of Human Major Transplantation Antigens
title_full	Studies on Biosynthesis, Assembly and Expression of Human Major Transplantation Antigens
title_fullStr	Studies on Biosynthesis, Assembly and Expression of Human Major Transplantation Antigens
title_full_unstemmed	Studies on Biosynthesis, Assembly and Expression of Human Major Transplantation Antigens
title_sort	studies on biosynthesis, assembly and expression of human major transplantation antigens
publishDate	1980
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00142956_v103_n1_p197_ALGRANATI http://hdl.handle.net/20.500.12110/paper_00142956_v103_n1_p197_ALGRANATI
_version_	1768543543817666560

Studies on Biosynthesis, Assembly and Expression of Human Major Transplantation Antigens

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