Effects of airborne particulate matter on alternative pre-mRNA splicing in colon cancer cells

Alternative pre-mRNA splicing plays key roles in determining tissue- and species-specific cell differentiation as well as in the onset of hereditary disease and cancer, being controlled by multiple post- and co-transcriptional regulatory mechanisms. We report here that airborne particulate matter, r...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Petrillo, Ezequiel, Allo, Mariano, Lafaille, Celina, Muñoz, Manuel Javier, Kornblihtt, Alberto Rodolfo
Publicado:	2015
Materias:	Air pollution Alternative splicing Bone morphogenetic protein Colon cancer cells Particulate matter bone morphogenetic protein messenger RNA bone morphogenetic protein 4 particulate matter primer DNA RNA precursor atmospheric pollution cancer cytology industrial waste protein RNA 5' untranslated region air pollution alternative RNA splicing Article cancer cell culture cancer cell line cell proliferation colon cancer colon carcinogenesis controlled study human human cell priority journal transcription elongation colon tumor drug effects genetics HEK293 cell line nucleotide sequence pathology pharmacology reverse transcription polymerase chain reaction tumor cell line Alternative Splicing Base Sequence Bone Morphogenetic Protein 4 Cell Line, Tumor Colonic Neoplasms DNA Primers HEK293 Cells Humans Particulate Matter Reverse Transcriptase Polymerase Chain Reaction RNA Precursors RNA, Messenger
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00139351_v140_n_p185_Buggiano http://hdl.handle.net/20.500.12110/paper_00139351_v140_n_p185_Buggiano
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00139351_v140_n_p185_Buggiano
record_format	dspace
spelling	paper:paper_00139351_v140_n_p185_Buggiano2023-06-08T14:36:28Z Effects of airborne particulate matter on alternative pre-mRNA splicing in colon cancer cells Petrillo, Ezequiel Allo, Mariano Lafaille, Celina Muñoz, Manuel Javier Kornblihtt, Alberto Rodolfo Air pollution Alternative splicing Bone morphogenetic protein Colon cancer cells Particulate matter bone morphogenetic protein messenger RNA bone morphogenetic protein 4 messenger RNA particulate matter primer DNA RNA precursor atmospheric pollution cancer cytology industrial waste particulate matter protein RNA 5' untranslated region air pollution alternative RNA splicing Article cancer cell culture cancer cell line cell proliferation colon cancer colon carcinogenesis controlled study human human cell particulate matter priority journal transcription elongation alternative RNA splicing colon tumor drug effects genetics HEK293 cell line nucleotide sequence particulate matter pathology pharmacology reverse transcription polymerase chain reaction tumor cell line Alternative Splicing Base Sequence Bone Morphogenetic Protein 4 Cell Line, Tumor Colonic Neoplasms DNA Primers HEK293 Cells Humans Particulate Matter Reverse Transcriptase Polymerase Chain Reaction RNA Precursors RNA, Messenger Alternative pre-mRNA splicing plays key roles in determining tissue- and species-specific cell differentiation as well as in the onset of hereditary disease and cancer, being controlled by multiple post- and co-transcriptional regulatory mechanisms. We report here that airborne particulate matter, resulting from industrial pollution, inhibits expression and specifically affects alternative splicing at the 5' untranslated region of the mRNA encoding the bone morphogenetic protein BMP4 in human colon cells in culture. These effects are consistent with a previously reported role for BMP4 in preventing colon cancer development, suggesting that ingestion of particulate matter could contribute to the onset of colon cell proliferation. We also show that the underlying mechanism might involve changes in transcriptional elongation. This is the first study to demonstrate that particulate matter causes non-pleiotropic changes in alternative splicing. © 2015 Elsevier Inc. Fil:Petrillo, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Alló, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lafaille, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Muñoz, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Kornblihtt, A.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2015 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00139351_v140_n_p185_Buggiano http://hdl.handle.net/20.500.12110/paper_00139351_v140_n_p185_Buggiano
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Air pollution Alternative splicing Bone morphogenetic protein Colon cancer cells Particulate matter bone morphogenetic protein messenger RNA bone morphogenetic protein 4 messenger RNA particulate matter primer DNA RNA precursor atmospheric pollution cancer cytology industrial waste particulate matter protein RNA 5' untranslated region air pollution alternative RNA splicing Article cancer cell culture cancer cell line cell proliferation colon cancer colon carcinogenesis controlled study human human cell particulate matter priority journal transcription elongation alternative RNA splicing colon tumor drug effects genetics HEK293 cell line nucleotide sequence particulate matter pathology pharmacology reverse transcription polymerase chain reaction tumor cell line Alternative Splicing Base Sequence Bone Morphogenetic Protein 4 Cell Line, Tumor Colonic Neoplasms DNA Primers HEK293 Cells Humans Particulate Matter Reverse Transcriptase Polymerase Chain Reaction RNA Precursors RNA, Messenger
spellingShingle	Air pollution Alternative splicing Bone morphogenetic protein Colon cancer cells Particulate matter bone morphogenetic protein messenger RNA bone morphogenetic protein 4 messenger RNA particulate matter primer DNA RNA precursor atmospheric pollution cancer cytology industrial waste particulate matter protein RNA 5' untranslated region air pollution alternative RNA splicing Article cancer cell culture cancer cell line cell proliferation colon cancer colon carcinogenesis controlled study human human cell particulate matter priority journal transcription elongation alternative RNA splicing colon tumor drug effects genetics HEK293 cell line nucleotide sequence particulate matter pathology pharmacology reverse transcription polymerase chain reaction tumor cell line Alternative Splicing Base Sequence Bone Morphogenetic Protein 4 Cell Line, Tumor Colonic Neoplasms DNA Primers HEK293 Cells Humans Particulate Matter Reverse Transcriptase Polymerase Chain Reaction RNA Precursors RNA, Messenger Petrillo, Ezequiel Allo, Mariano Lafaille, Celina Muñoz, Manuel Javier Kornblihtt, Alberto Rodolfo Effects of airborne particulate matter on alternative pre-mRNA splicing in colon cancer cells
topic_facet	Air pollution Alternative splicing Bone morphogenetic protein Colon cancer cells Particulate matter bone morphogenetic protein messenger RNA bone morphogenetic protein 4 messenger RNA particulate matter primer DNA RNA precursor atmospheric pollution cancer cytology industrial waste particulate matter protein RNA 5' untranslated region air pollution alternative RNA splicing Article cancer cell culture cancer cell line cell proliferation colon cancer colon carcinogenesis controlled study human human cell particulate matter priority journal transcription elongation alternative RNA splicing colon tumor drug effects genetics HEK293 cell line nucleotide sequence particulate matter pathology pharmacology reverse transcription polymerase chain reaction tumor cell line Alternative Splicing Base Sequence Bone Morphogenetic Protein 4 Cell Line, Tumor Colonic Neoplasms DNA Primers HEK293 Cells Humans Particulate Matter Reverse Transcriptase Polymerase Chain Reaction RNA Precursors RNA, Messenger
description	Alternative pre-mRNA splicing plays key roles in determining tissue- and species-specific cell differentiation as well as in the onset of hereditary disease and cancer, being controlled by multiple post- and co-transcriptional regulatory mechanisms. We report here that airborne particulate matter, resulting from industrial pollution, inhibits expression and specifically affects alternative splicing at the 5' untranslated region of the mRNA encoding the bone morphogenetic protein BMP4 in human colon cells in culture. These effects are consistent with a previously reported role for BMP4 in preventing colon cancer development, suggesting that ingestion of particulate matter could contribute to the onset of colon cell proliferation. We also show that the underlying mechanism might involve changes in transcriptional elongation. This is the first study to demonstrate that particulate matter causes non-pleiotropic changes in alternative splicing. © 2015 Elsevier Inc.
author	Petrillo, Ezequiel Allo, Mariano Lafaille, Celina Muñoz, Manuel Javier Kornblihtt, Alberto Rodolfo
author_facet	Petrillo, Ezequiel Allo, Mariano Lafaille, Celina Muñoz, Manuel Javier Kornblihtt, Alberto Rodolfo
author_sort	Petrillo, Ezequiel
title	Effects of airborne particulate matter on alternative pre-mRNA splicing in colon cancer cells
title_short	Effects of airborne particulate matter on alternative pre-mRNA splicing in colon cancer cells
title_full	Effects of airborne particulate matter on alternative pre-mRNA splicing in colon cancer cells
title_fullStr	Effects of airborne particulate matter on alternative pre-mRNA splicing in colon cancer cells
title_full_unstemmed	Effects of airborne particulate matter on alternative pre-mRNA splicing in colon cancer cells
title_sort	effects of airborne particulate matter on alternative pre-mrna splicing in colon cancer cells
publishDate	2015
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00139351_v140_n_p185_Buggiano http://hdl.handle.net/20.500.12110/paper_00139351_v140_n_p185_Buggiano
work_keys_str_mv	AT petrilloezequiel effectsofairborneparticulatematteronalternativepremrnasplicingincoloncancercells AT allomariano effectsofairborneparticulatematteronalternativepremrnasplicingincoloncancercells AT lafaillecelina effectsofairborneparticulatematteronalternativepremrnasplicingincoloncancercells AT munozmanueljavier effectsofairborneparticulatematteronalternativepremrnasplicingincoloncancercells AT kornblihttalbertorodolfo effectsofairborneparticulatematteronalternativepremrnasplicingincoloncancercells
_version_	1768542818288009216

Effects of airborne particulate matter on alternative pre-mRNA splicing in colon cancer cells

Ejemplares similares