Active and total transforming growth factor-β1 are differentially regulated by dopamine and estradiol in the pituitary
Dopamine, acting through the dopamine type 2 receptor (Drd2), is the main inhibitor of pituitary prolactin (PRL) secretion and lactotroph proliferation. TGF-β1 is involved, at least in part, in mediating these actions. It was described that TGF-β1 synthesis in rat pituitary lactotrophs is up-regulat...
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2011
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00137227_v152_n7_p2722_Recouvreux http://hdl.handle.net/20.500.12110/paper_00137227_v152_n7_p2722_Recouvreux |
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paper:paper_00137227_v152_n7_p2722_Recouvreux2023-06-08T14:36:15Z Active and total transforming growth factor-β1 are differentially regulated by dopamine and estradiol in the pituitary dopamine dopamine 2 receptor estradiol messenger RNA prolactin sulpiride transforming growth factor beta1 transforming growth factor beta2 allele animal cell animal experiment article cell proliferation female hypophysis hypophysis cell in vitro study in vivo study mouse nonhuman priority journal prolactin secreting cell Animals Cell Proliferation Cells, Cultured Dopamine Agonists Dopamine Antagonists Estradiol Female Gene Expression Regulation Hyperprolactinemia Lactotrophs Mice Mice, 129 Strain Mice, Inbred C57BL Mice, Transgenic Pituitary Gland, Anterior Prolactin Protein-Serine-Threonine Kinases Receptors, Dopamine D2 Receptors, Transforming Growth Factor beta RNA, Messenger Transforming Growth Factor beta1 Dopamine, acting through the dopamine type 2 receptor (Drd2), is the main inhibitor of pituitary prolactin (PRL) secretion and lactotroph proliferation. TGF-β1 is involved, at least in part, in mediating these actions. It was described that TGF-β1 synthesis in rat pituitary lactotrophs is up-regulated by dopamine and down-regulated by estradiol. TGF-β1 is secreted as a large latent complex. The local regulation of cytokine activation in the pituitary has not yet been explored. In this work, we studied pituitary active and total TGF-β1 content, as well as TGF-β1 mRNA, and the in vivo role of dopamine and estradiol on pituitary TGF-β1 levels. Adult female mice (wild type), and female mice with a null mutation in the Drd2 (Drd2-/-), were used. The loss of dopaminergic tone induced a decrease in TGF-β1 mRNA expression, in active and total cytokine content, and in TGF-β type II receptor expression. Dopamine regulation of pituitary TGF-β1 activation process was inferred by the inhibition of active cytokine by in vivo sulpiride treatment. Interestingly, in the absence of dopaminergic tone, estradiol induced a strong increase in active TGF-β1. PRL secretion correlated with active, but not total cytokine. TGF-β1 inhibitory action on lactotroph proliferation and PRL secretion was decreased in Drd2-/- pituitary cells, in correlation with decreased TGF-β type II receptor. The study of the TGF-β1 activation process and its regulation is essential to understand the cytokine activity. As an intermediary of dopamine inhibition of lactotroph function, TGF-β1 and local activators may be important targets in the treatment of dopamine agonist-resistant prolactinomas. Copyright © 2011 by The Endocrine Society. 2011 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00137227_v152_n7_p2722_Recouvreux http://hdl.handle.net/20.500.12110/paper_00137227_v152_n7_p2722_Recouvreux |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
dopamine dopamine 2 receptor estradiol messenger RNA prolactin sulpiride transforming growth factor beta1 transforming growth factor beta2 allele animal cell animal experiment article cell proliferation female hypophysis hypophysis cell in vitro study in vivo study mouse nonhuman priority journal prolactin secreting cell Animals Cell Proliferation Cells, Cultured Dopamine Agonists Dopamine Antagonists Estradiol Female Gene Expression Regulation Hyperprolactinemia Lactotrophs Mice Mice, 129 Strain Mice, Inbred C57BL Mice, Transgenic Pituitary Gland, Anterior Prolactin Protein-Serine-Threonine Kinases Receptors, Dopamine D2 Receptors, Transforming Growth Factor beta RNA, Messenger Transforming Growth Factor beta1 |
| spellingShingle |
dopamine dopamine 2 receptor estradiol messenger RNA prolactin sulpiride transforming growth factor beta1 transforming growth factor beta2 allele animal cell animal experiment article cell proliferation female hypophysis hypophysis cell in vitro study in vivo study mouse nonhuman priority journal prolactin secreting cell Animals Cell Proliferation Cells, Cultured Dopamine Agonists Dopamine Antagonists Estradiol Female Gene Expression Regulation Hyperprolactinemia Lactotrophs Mice Mice, 129 Strain Mice, Inbred C57BL Mice, Transgenic Pituitary Gland, Anterior Prolactin Protein-Serine-Threonine Kinases Receptors, Dopamine D2 Receptors, Transforming Growth Factor beta RNA, Messenger Transforming Growth Factor beta1 Active and total transforming growth factor-β1 are differentially regulated by dopamine and estradiol in the pituitary |
| topic_facet |
dopamine dopamine 2 receptor estradiol messenger RNA prolactin sulpiride transforming growth factor beta1 transforming growth factor beta2 allele animal cell animal experiment article cell proliferation female hypophysis hypophysis cell in vitro study in vivo study mouse nonhuman priority journal prolactin secreting cell Animals Cell Proliferation Cells, Cultured Dopamine Agonists Dopamine Antagonists Estradiol Female Gene Expression Regulation Hyperprolactinemia Lactotrophs Mice Mice, 129 Strain Mice, Inbred C57BL Mice, Transgenic Pituitary Gland, Anterior Prolactin Protein-Serine-Threonine Kinases Receptors, Dopamine D2 Receptors, Transforming Growth Factor beta RNA, Messenger Transforming Growth Factor beta1 |
| description |
Dopamine, acting through the dopamine type 2 receptor (Drd2), is the main inhibitor of pituitary prolactin (PRL) secretion and lactotroph proliferation. TGF-β1 is involved, at least in part, in mediating these actions. It was described that TGF-β1 synthesis in rat pituitary lactotrophs is up-regulated by dopamine and down-regulated by estradiol. TGF-β1 is secreted as a large latent complex. The local regulation of cytokine activation in the pituitary has not yet been explored. In this work, we studied pituitary active and total TGF-β1 content, as well as TGF-β1 mRNA, and the in vivo role of dopamine and estradiol on pituitary TGF-β1 levels. Adult female mice (wild type), and female mice with a null mutation in the Drd2 (Drd2-/-), were used. The loss of dopaminergic tone induced a decrease in TGF-β1 mRNA expression, in active and total cytokine content, and in TGF-β type II receptor expression. Dopamine regulation of pituitary TGF-β1 activation process was inferred by the inhibition of active cytokine by in vivo sulpiride treatment. Interestingly, in the absence of dopaminergic tone, estradiol induced a strong increase in active TGF-β1. PRL secretion correlated with active, but not total cytokine. TGF-β1 inhibitory action on lactotroph proliferation and PRL secretion was decreased in Drd2-/- pituitary cells, in correlation with decreased TGF-β type II receptor. The study of the TGF-β1 activation process and its regulation is essential to understand the cytokine activity. As an intermediary of dopamine inhibition of lactotroph function, TGF-β1 and local activators may be important targets in the treatment of dopamine agonist-resistant prolactinomas. Copyright © 2011 by The Endocrine Society. |
| title |
Active and total transforming growth factor-β1 are differentially regulated by dopamine and estradiol in the pituitary |
| title_short |
Active and total transforming growth factor-β1 are differentially regulated by dopamine and estradiol in the pituitary |
| title_full |
Active and total transforming growth factor-β1 are differentially regulated by dopamine and estradiol in the pituitary |
| title_fullStr |
Active and total transforming growth factor-β1 are differentially regulated by dopamine and estradiol in the pituitary |
| title_full_unstemmed |
Active and total transforming growth factor-β1 are differentially regulated by dopamine and estradiol in the pituitary |
| title_sort |
active and total transforming growth factor-β1 are differentially regulated by dopamine and estradiol in the pituitary |
| publishDate |
2011 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00137227_v152_n7_p2722_Recouvreux http://hdl.handle.net/20.500.12110/paper_00137227_v152_n7_p2722_Recouvreux |
| _version_ |
1768545309808394240 |