Injury elicited increase in spinal cord neurosteroid content analyzed by gas chromatography mass spectrometry
The effects of spinal cord injury (SCI), combined with castration and adrenalectomy, and of progesterone (PROG) treatment on neurosteroid levels and steroidogenic enzyme expression were investigated in the adult male rat spinal cord (SC). Steroid levels were quantified by gas chromatography/mass spe...
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2006
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00137227_v147_n4_p1847_Labombarda http://hdl.handle.net/20.500.12110/paper_00137227_v147_n4_p1847_Labombarda |
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paper:paper_00137227_v147_n4_p1847_Labombarda2023-06-08T14:36:13Z Injury elicited increase in spinal cord neurosteroid content analyzed by gas chromatography mass spectrometry 3(or 17)beta hydroxysteroid dehydrogenase 3alpha hydroxy 5alpha pregnan 20 one 5alpha pregnane 3,20 dione cytochrome P450 messenger RNA neurosteroid pregnenolone progesterone steroid delta isomerase adrenalectomy animal experiment article biosynthesis controlled study gas chromatography gonadectomy male mass spectrometry nonhuman priority journal quantitative analysis rat reverse transcription polymerase chain reaction spinal cord 17-Hydroxysteroid Dehydrogenases 5-alpha-Dihydroprogesterone Animals Cholesterol Side-Chain Cleavage Enzyme Gas Chromatography-Mass Spectrometry Male Pregnanolone Pregnenolone Progesterone Rats Rats, Sprague-Dawley Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger Spinal Cord Spinal Cord Injuries The effects of spinal cord injury (SCI), combined with castration and adrenalectomy, and of progesterone (PROG) treatment on neurosteroid levels and steroidogenic enzyme expression were investigated in the adult male rat spinal cord (SC). Steroid levels were quantified by gas chromatography/mass spectrometry in SC and plasma, and mRNAs of enzymes by quantitative real-time RT-PCR. The levels of pregnenolone (PREG), PROG, 5α-dihydroprogesterone, 3α,5α-tetrahydroprogesterone increased in SC 75 h after transection without significant increase in the plasma. After combined adrenalectomy and gonadectomy, significant levels of PREG and PROG remained in the SC, suggesting their local biosynthesis. In the SC of adrenalectomized and gonadectomized rats, there was an increase of PREG 24 h after SCI, followed at 75 h by a concomitant increase in its direct metabolite, PROG. These observations are consistent with a sequential increase of PREG biosynthesis and its conversion to PROG within the SC in response to injury. However, no significant change in P450-side chain cleavage and 3β-hydroxysteroid dehydrogenase/Δ5-Δ4 isomerase mRNA levels was observed after SCI. Systemic PROG treatment after SCI, resulted in a very large increase in PROG, 5α-dihydroprogesterone, and 3α,5α-tetrahydroprogesterone in both plasma and SC. Furthermore, high levels of 3β,5α-tetrahydroprogesterone were detected in SC, whereas their plasma levels remained barely detectable. Because the ratio of reduced metabolites to PROG was 65-times higher in SC than in the plasma, it appears likely that reduced metabolites mainly originated from local biosynthesis. Our results strongly suggest an important role for locally biosynthesized neurosteroids in the response of the SC to injury. Copyright © 2006 by The Endocrine Society. 2006 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00137227_v147_n4_p1847_Labombarda http://hdl.handle.net/20.500.12110/paper_00137227_v147_n4_p1847_Labombarda |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
3(or 17)beta hydroxysteroid dehydrogenase 3alpha hydroxy 5alpha pregnan 20 one 5alpha pregnane 3,20 dione cytochrome P450 messenger RNA neurosteroid pregnenolone progesterone steroid delta isomerase adrenalectomy animal experiment article biosynthesis controlled study gas chromatography gonadectomy male mass spectrometry nonhuman priority journal quantitative analysis rat reverse transcription polymerase chain reaction spinal cord 17-Hydroxysteroid Dehydrogenases 5-alpha-Dihydroprogesterone Animals Cholesterol Side-Chain Cleavage Enzyme Gas Chromatography-Mass Spectrometry Male Pregnanolone Pregnenolone Progesterone Rats Rats, Sprague-Dawley Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger Spinal Cord Spinal Cord Injuries |
| spellingShingle |
3(or 17)beta hydroxysteroid dehydrogenase 3alpha hydroxy 5alpha pregnan 20 one 5alpha pregnane 3,20 dione cytochrome P450 messenger RNA neurosteroid pregnenolone progesterone steroid delta isomerase adrenalectomy animal experiment article biosynthesis controlled study gas chromatography gonadectomy male mass spectrometry nonhuman priority journal quantitative analysis rat reverse transcription polymerase chain reaction spinal cord 17-Hydroxysteroid Dehydrogenases 5-alpha-Dihydroprogesterone Animals Cholesterol Side-Chain Cleavage Enzyme Gas Chromatography-Mass Spectrometry Male Pregnanolone Pregnenolone Progesterone Rats Rats, Sprague-Dawley Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger Spinal Cord Spinal Cord Injuries Injury elicited increase in spinal cord neurosteroid content analyzed by gas chromatography mass spectrometry |
| topic_facet |
3(or 17)beta hydroxysteroid dehydrogenase 3alpha hydroxy 5alpha pregnan 20 one 5alpha pregnane 3,20 dione cytochrome P450 messenger RNA neurosteroid pregnenolone progesterone steroid delta isomerase adrenalectomy animal experiment article biosynthesis controlled study gas chromatography gonadectomy male mass spectrometry nonhuman priority journal quantitative analysis rat reverse transcription polymerase chain reaction spinal cord 17-Hydroxysteroid Dehydrogenases 5-alpha-Dihydroprogesterone Animals Cholesterol Side-Chain Cleavage Enzyme Gas Chromatography-Mass Spectrometry Male Pregnanolone Pregnenolone Progesterone Rats Rats, Sprague-Dawley Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger Spinal Cord Spinal Cord Injuries |
| description |
The effects of spinal cord injury (SCI), combined with castration and adrenalectomy, and of progesterone (PROG) treatment on neurosteroid levels and steroidogenic enzyme expression were investigated in the adult male rat spinal cord (SC). Steroid levels were quantified by gas chromatography/mass spectrometry in SC and plasma, and mRNAs of enzymes by quantitative real-time RT-PCR. The levels of pregnenolone (PREG), PROG, 5α-dihydroprogesterone, 3α,5α-tetrahydroprogesterone increased in SC 75 h after transection without significant increase in the plasma. After combined adrenalectomy and gonadectomy, significant levels of PREG and PROG remained in the SC, suggesting their local biosynthesis. In the SC of adrenalectomized and gonadectomized rats, there was an increase of PREG 24 h after SCI, followed at 75 h by a concomitant increase in its direct metabolite, PROG. These observations are consistent with a sequential increase of PREG biosynthesis and its conversion to PROG within the SC in response to injury. However, no significant change in P450-side chain cleavage and 3β-hydroxysteroid dehydrogenase/Δ5-Δ4 isomerase mRNA levels was observed after SCI. Systemic PROG treatment after SCI, resulted in a very large increase in PROG, 5α-dihydroprogesterone, and 3α,5α-tetrahydroprogesterone in both plasma and SC. Furthermore, high levels of 3β,5α-tetrahydroprogesterone were detected in SC, whereas their plasma levels remained barely detectable. Because the ratio of reduced metabolites to PROG was 65-times higher in SC than in the plasma, it appears likely that reduced metabolites mainly originated from local biosynthesis. Our results strongly suggest an important role for locally biosynthesized neurosteroids in the response of the SC to injury. Copyright © 2006 by The Endocrine Society. |
| title |
Injury elicited increase in spinal cord neurosteroid content analyzed by gas chromatography mass spectrometry |
| title_short |
Injury elicited increase in spinal cord neurosteroid content analyzed by gas chromatography mass spectrometry |
| title_full |
Injury elicited increase in spinal cord neurosteroid content analyzed by gas chromatography mass spectrometry |
| title_fullStr |
Injury elicited increase in spinal cord neurosteroid content analyzed by gas chromatography mass spectrometry |
| title_full_unstemmed |
Injury elicited increase in spinal cord neurosteroid content analyzed by gas chromatography mass spectrometry |
| title_sort |
injury elicited increase in spinal cord neurosteroid content analyzed by gas chromatography mass spectrometry |
| publishDate |
2006 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00137227_v147_n4_p1847_Labombarda http://hdl.handle.net/20.500.12110/paper_00137227_v147_n4_p1847_Labombarda |
| _version_ |
1768542868585054208 |