Reduced expression of the cytokine transducer gp130 inhibits hormone secretion, cell growth, and tumor development of pituitary lactosomatotrophic GH3 cells

Two of the most potent cytokines that regulate anterior pituitary cell function are leukemia inhibitory factor and IL-6. These and others like IL-11 and ciliary neurotrophic factor are referred to as the gp130 cytokines because they share the gp130 glycoprotein as a common receptor initial signal tr...

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Autores principales:	Perez Castro, Carolina Inés, Giacomini, Damiana Paula, Carbia Nagashima, Alberto Nicolás, Graciarena, Mariana, Kobayashi, Ken, Páez Pereda, Marcelo
Publicado:	2003
Materias:	antisense oligodeoxynucleotide complementary DNA cytokine glycoprotein gp 130 interleukin 11 interleukin 6 leukemia inhibitory factor adenohypophysis animal cell article carcinogenesis cell function cell growth cell proliferation cell strain GH3 clone controlled study gene expression gene overexpression genetic transfection growth hormone release hormone release hypophysis cell nonhuman nude mouse priority journal prolactin release prolactin synthesis rat signal transduction transcription initiation Animals Antigens, CD Cell Division Cytokine Receptor gp130 Cytokines Gene Expression Regulation, Neoplastic Hormones Membrane Glycoproteins Mice Mice, Nude Neoplasm Transplantation Pituitary Neoplasms Rats RNA, Messenger Tumor Cells, Cultured
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00137227_v144_n2_p693_PerezCastro http://hdl.handle.net/20.500.12110/paper_00137227_v144_n2_p693_PerezCastro
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	paper:paper_00137227_v144_n2_p693_PerezCastro2023-06-08T14:36:10Z Reduced expression of the cytokine transducer gp130 inhibits hormone secretion, cell growth, and tumor development of pituitary lactosomatotrophic GH3 cells Perez Castro, Carolina Inés Giacomini, Damiana Paula Carbia Nagashima, Alberto Nicolás Graciarena, Mariana Kobayashi, Ken Páez Pereda, Marcelo antisense oligodeoxynucleotide complementary DNA cytokine glycoprotein gp 130 interleukin 11 interleukin 6 leukemia inhibitory factor adenohypophysis animal cell article carcinogenesis cell function cell growth cell proliferation cell strain GH3 clone controlled study gene expression gene overexpression genetic transfection growth hormone release hormone release hypophysis cell nonhuman nude mouse priority journal prolactin release prolactin synthesis rat signal transduction transcription initiation Animals Antigens, CD Cell Division Cytokine Receptor gp130 Cytokines Gene Expression Regulation, Neoplastic Hormones Membrane Glycoproteins Mice Mice, Nude Neoplasm Transplantation Pituitary Neoplasms Rats RNA, Messenger Tumor Cells, Cultured Two of the most potent cytokines that regulate anterior pituitary cell function are leukemia inhibitory factor and IL-6. These and others like IL-11 and ciliary neurotrophic factor are referred to as the gp130 cytokines because they share the gp130 glycoprotein as a common receptor initial signal transducer. We and others have shown that gp130 cytokines and their receptors are expressed and functional in normal and tumoral anterior pituitary cells. To study the role of gp130 cytokines in tumorigenic process, we generated gp130 cDNA gp130 sense and gp130 antisense (gp130-AS) transfected stable clones derived from lactosomatotroph GH3 cells. We examined hormone secretion and cell proliferation of these clones as well as their tumorigenic properties in athymic nude mice. Although gp130-AS clones, which have low gp130 levels and impaired signal transducer and activator of transcription 3 activity and suppressor of cytokine signaling-3 expression, showed reduced proliferation and hormone secretion (GH and prolactin) in response to gp130 cytokines, they had a normal response to gp130-independent stimuli. Moreover, gp130-AS clones showed a severely impaired in vivo tumor development. In contrast, the overexpressing gp130 clones (gp130 sense) showed no differences, compared with cells transfected with control vector. Thus, the present study provides new evidence supporting a link between gp130 and pituitary abnormal growth. Fil:Perez Castro, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Giacomini, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Carbia Nagashima, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Graciarena, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Kobayashi, K. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Páez-Pereda, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2003 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00137227_v144_n2_p693_PerezCastro http://hdl.handle.net/20.500.12110/paper_00137227_v144_n2_p693_PerezCastro
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	antisense oligodeoxynucleotide complementary DNA cytokine glycoprotein gp 130 interleukin 11 interleukin 6 leukemia inhibitory factor adenohypophysis animal cell article carcinogenesis cell function cell growth cell proliferation cell strain GH3 clone controlled study gene expression gene overexpression genetic transfection growth hormone release hormone release hypophysis cell nonhuman nude mouse priority journal prolactin release prolactin synthesis rat signal transduction transcription initiation Animals Antigens, CD Cell Division Cytokine Receptor gp130 Cytokines Gene Expression Regulation, Neoplastic Hormones Membrane Glycoproteins Mice Mice, Nude Neoplasm Transplantation Pituitary Neoplasms Rats RNA, Messenger Tumor Cells, Cultured
spellingShingle	antisense oligodeoxynucleotide complementary DNA cytokine glycoprotein gp 130 interleukin 11 interleukin 6 leukemia inhibitory factor adenohypophysis animal cell article carcinogenesis cell function cell growth cell proliferation cell strain GH3 clone controlled study gene expression gene overexpression genetic transfection growth hormone release hormone release hypophysis cell nonhuman nude mouse priority journal prolactin release prolactin synthesis rat signal transduction transcription initiation Animals Antigens, CD Cell Division Cytokine Receptor gp130 Cytokines Gene Expression Regulation, Neoplastic Hormones Membrane Glycoproteins Mice Mice, Nude Neoplasm Transplantation Pituitary Neoplasms Rats RNA, Messenger Tumor Cells, Cultured Perez Castro, Carolina Inés Giacomini, Damiana Paula Carbia Nagashima, Alberto Nicolás Graciarena, Mariana Kobayashi, Ken Páez Pereda, Marcelo Reduced expression of the cytokine transducer gp130 inhibits hormone secretion, cell growth, and tumor development of pituitary lactosomatotrophic GH3 cells
topic_facet	antisense oligodeoxynucleotide complementary DNA cytokine glycoprotein gp 130 interleukin 11 interleukin 6 leukemia inhibitory factor adenohypophysis animal cell article carcinogenesis cell function cell growth cell proliferation cell strain GH3 clone controlled study gene expression gene overexpression genetic transfection growth hormone release hormone release hypophysis cell nonhuman nude mouse priority journal prolactin release prolactin synthesis rat signal transduction transcription initiation Animals Antigens, CD Cell Division Cytokine Receptor gp130 Cytokines Gene Expression Regulation, Neoplastic Hormones Membrane Glycoproteins Mice Mice, Nude Neoplasm Transplantation Pituitary Neoplasms Rats RNA, Messenger Tumor Cells, Cultured
description	Two of the most potent cytokines that regulate anterior pituitary cell function are leukemia inhibitory factor and IL-6. These and others like IL-11 and ciliary neurotrophic factor are referred to as the gp130 cytokines because they share the gp130 glycoprotein as a common receptor initial signal transducer. We and others have shown that gp130 cytokines and their receptors are expressed and functional in normal and tumoral anterior pituitary cells. To study the role of gp130 cytokines in tumorigenic process, we generated gp130 cDNA gp130 sense and gp130 antisense (gp130-AS) transfected stable clones derived from lactosomatotroph GH3 cells. We examined hormone secretion and cell proliferation of these clones as well as their tumorigenic properties in athymic nude mice. Although gp130-AS clones, which have low gp130 levels and impaired signal transducer and activator of transcription 3 activity and suppressor of cytokine signaling-3 expression, showed reduced proliferation and hormone secretion (GH and prolactin) in response to gp130 cytokines, they had a normal response to gp130-independent stimuli. Moreover, gp130-AS clones showed a severely impaired in vivo tumor development. In contrast, the overexpressing gp130 clones (gp130 sense) showed no differences, compared with cells transfected with control vector. Thus, the present study provides new evidence supporting a link between gp130 and pituitary abnormal growth.
author	Perez Castro, Carolina Inés Giacomini, Damiana Paula Carbia Nagashima, Alberto Nicolás Graciarena, Mariana Kobayashi, Ken Páez Pereda, Marcelo
author_facet	Perez Castro, Carolina Inés Giacomini, Damiana Paula Carbia Nagashima, Alberto Nicolás Graciarena, Mariana Kobayashi, Ken Páez Pereda, Marcelo
author_sort	Perez Castro, Carolina Inés
title	Reduced expression of the cytokine transducer gp130 inhibits hormone secretion, cell growth, and tumor development of pituitary lactosomatotrophic GH3 cells
title_short	Reduced expression of the cytokine transducer gp130 inhibits hormone secretion, cell growth, and tumor development of pituitary lactosomatotrophic GH3 cells
title_full	Reduced expression of the cytokine transducer gp130 inhibits hormone secretion, cell growth, and tumor development of pituitary lactosomatotrophic GH3 cells
title_fullStr	Reduced expression of the cytokine transducer gp130 inhibits hormone secretion, cell growth, and tumor development of pituitary lactosomatotrophic GH3 cells
title_full_unstemmed	Reduced expression of the cytokine transducer gp130 inhibits hormone secretion, cell growth, and tumor development of pituitary lactosomatotrophic GH3 cells
title_sort	reduced expression of the cytokine transducer gp130 inhibits hormone secretion, cell growth, and tumor development of pituitary lactosomatotrophic gh3 cells
publishDate	2003
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00137227_v144_n2_p693_PerezCastro http://hdl.handle.net/20.500.12110/paper_00137227_v144_n2_p693_PerezCastro
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_version_	1768546003476021248

Reduced expression of the cytokine transducer gp130 inhibits hormone secretion, cell growth, and tumor development of pituitary lactosomatotrophic GH3 cells

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