Transforming growth factor-β stimulates vascular endothelial growth factor production by folliculostellate pituitary cells
TGF-β isoforms are expressed in the anterior pituitary and modulate the growth and function of endocrine pituitary cells. Recently, TGF-β has been shown to stimulate growth and basic fibroblast growth factor secretion in nonendocrine folliculostellate (FS) pituitary cells. We therefore studied wheth...
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2002
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00137227_v143_n10_p3759_Renner http://hdl.handle.net/20.500.12110/paper_00137227_v143_n10_p3759_Renner |
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paper:paper_00137227_v143_n10_p3759_Renner2023-06-08T14:36:09Z Transforming growth factor-β stimulates vascular endothelial growth factor production by folliculostellate pituitary cells cycloheximide dexamethasone messenger RNA Smad2 protein Smad3 protein Smad4 protein transforming growth factor beta receptor transforming growth factor beta1 transforming growth factor beta2 transforming growth factor beta3 vasculotropin angiogenesis animal cell article blood vessel permeability cell nucleus controlled study hypophysis cell hypophysis tumor mouse nonhuman priority journal protein analysis protein expression protein transport rat reverse transcription polymerase chain reaction Animals Biological Transport Cell Nucleus Cells, Cultured Dexamethasone DNA-Binding Proteins Endothelial Growth Factors Glucocorticoids Lymphokines Male Mice Pituitary Gland Pituitary Gland, Anterior Protein Isoforms Rats Rats, Sprague-Dawley Receptors, Transforming Growth Factor beta RNA, Messenger Smad2 Protein Trans-Activators Transforming Growth Factor beta Vascular Endothelial Growth Factor A Vascular Endothelial Growth Factors TGF-β isoforms are expressed in the anterior pituitary and modulate the growth and function of endocrine pituitary cells. Recently, TGF-β has been shown to stimulate growth and basic fibroblast growth factor secretion in nonendocrine folliculostellate (FS) pituitary cells. We therefore studied whether the production of FS cell-derived vascular endothelial growth factor (VEGF), the most important regulator of vascular permeability and angiogenesis, is affected by TGF-β. We observed by RT-PCR that TtT/GF cells, which are FS mouse pituitary tumor cells, synthesize TGF-β1, -β2, and -β3. They also express TGF-β receptors types 1 and 2, as well as Smad2, Smad3, and Smad4 proteins, which are essential for TGF-β binding and signaling. Stimulation of TtT/GF cells with either TGF-β1 or TGF-β3 induced a rapid translocation of Smad2 into the cell nuclei. Both TGF-β isoforms dose dependently stimulated VEGF production in TtT/GF cells, but not in lactosomatotroph GH3 cells. Time-course studies and suppression of TGF-β-induced VEGF production by cycloheximide suggest that TGF-β induces de novo synthesis of VEGF in folliculostellate cells, which is completely blocked by dexamethasone. In primary rat pituitary cell cultures, TGF-β and -β3 stimulated VEGF production. TGF-β stimulation of VEGF production by folliculostellate cells could modulate intrapituitary vascular permeability and integrity as well as angiogenesis in an auto-/paracrine manner. 2002 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00137227_v143_n10_p3759_Renner http://hdl.handle.net/20.500.12110/paper_00137227_v143_n10_p3759_Renner |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
cycloheximide dexamethasone messenger RNA Smad2 protein Smad3 protein Smad4 protein transforming growth factor beta receptor transforming growth factor beta1 transforming growth factor beta2 transforming growth factor beta3 vasculotropin angiogenesis animal cell article blood vessel permeability cell nucleus controlled study hypophysis cell hypophysis tumor mouse nonhuman priority journal protein analysis protein expression protein transport rat reverse transcription polymerase chain reaction Animals Biological Transport Cell Nucleus Cells, Cultured Dexamethasone DNA-Binding Proteins Endothelial Growth Factors Glucocorticoids Lymphokines Male Mice Pituitary Gland Pituitary Gland, Anterior Protein Isoforms Rats Rats, Sprague-Dawley Receptors, Transforming Growth Factor beta RNA, Messenger Smad2 Protein Trans-Activators Transforming Growth Factor beta Vascular Endothelial Growth Factor A Vascular Endothelial Growth Factors |
| spellingShingle |
cycloheximide dexamethasone messenger RNA Smad2 protein Smad3 protein Smad4 protein transforming growth factor beta receptor transforming growth factor beta1 transforming growth factor beta2 transforming growth factor beta3 vasculotropin angiogenesis animal cell article blood vessel permeability cell nucleus controlled study hypophysis cell hypophysis tumor mouse nonhuman priority journal protein analysis protein expression protein transport rat reverse transcription polymerase chain reaction Animals Biological Transport Cell Nucleus Cells, Cultured Dexamethasone DNA-Binding Proteins Endothelial Growth Factors Glucocorticoids Lymphokines Male Mice Pituitary Gland Pituitary Gland, Anterior Protein Isoforms Rats Rats, Sprague-Dawley Receptors, Transforming Growth Factor beta RNA, Messenger Smad2 Protein Trans-Activators Transforming Growth Factor beta Vascular Endothelial Growth Factor A Vascular Endothelial Growth Factors Transforming growth factor-β stimulates vascular endothelial growth factor production by folliculostellate pituitary cells |
| topic_facet |
cycloheximide dexamethasone messenger RNA Smad2 protein Smad3 protein Smad4 protein transforming growth factor beta receptor transforming growth factor beta1 transforming growth factor beta2 transforming growth factor beta3 vasculotropin angiogenesis animal cell article blood vessel permeability cell nucleus controlled study hypophysis cell hypophysis tumor mouse nonhuman priority journal protein analysis protein expression protein transport rat reverse transcription polymerase chain reaction Animals Biological Transport Cell Nucleus Cells, Cultured Dexamethasone DNA-Binding Proteins Endothelial Growth Factors Glucocorticoids Lymphokines Male Mice Pituitary Gland Pituitary Gland, Anterior Protein Isoforms Rats Rats, Sprague-Dawley Receptors, Transforming Growth Factor beta RNA, Messenger Smad2 Protein Trans-Activators Transforming Growth Factor beta Vascular Endothelial Growth Factor A Vascular Endothelial Growth Factors |
| description |
TGF-β isoforms are expressed in the anterior pituitary and modulate the growth and function of endocrine pituitary cells. Recently, TGF-β has been shown to stimulate growth and basic fibroblast growth factor secretion in nonendocrine folliculostellate (FS) pituitary cells. We therefore studied whether the production of FS cell-derived vascular endothelial growth factor (VEGF), the most important regulator of vascular permeability and angiogenesis, is affected by TGF-β. We observed by RT-PCR that TtT/GF cells, which are FS mouse pituitary tumor cells, synthesize TGF-β1, -β2, and -β3. They also express TGF-β receptors types 1 and 2, as well as Smad2, Smad3, and Smad4 proteins, which are essential for TGF-β binding and signaling. Stimulation of TtT/GF cells with either TGF-β1 or TGF-β3 induced a rapid translocation of Smad2 into the cell nuclei. Both TGF-β isoforms dose dependently stimulated VEGF production in TtT/GF cells, but not in lactosomatotroph GH3 cells. Time-course studies and suppression of TGF-β-induced VEGF production by cycloheximide suggest that TGF-β induces de novo synthesis of VEGF in folliculostellate cells, which is completely blocked by dexamethasone. In primary rat pituitary cell cultures, TGF-β and -β3 stimulated VEGF production. TGF-β stimulation of VEGF production by folliculostellate cells could modulate intrapituitary vascular permeability and integrity as well as angiogenesis in an auto-/paracrine manner. |
| title |
Transforming growth factor-β stimulates vascular endothelial growth factor production by folliculostellate pituitary cells |
| title_short |
Transforming growth factor-β stimulates vascular endothelial growth factor production by folliculostellate pituitary cells |
| title_full |
Transforming growth factor-β stimulates vascular endothelial growth factor production by folliculostellate pituitary cells |
| title_fullStr |
Transforming growth factor-β stimulates vascular endothelial growth factor production by folliculostellate pituitary cells |
| title_full_unstemmed |
Transforming growth factor-β stimulates vascular endothelial growth factor production by folliculostellate pituitary cells |
| title_sort |
transforming growth factor-β stimulates vascular endothelial growth factor production by folliculostellate pituitary cells |
| publishDate |
2002 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00137227_v143_n10_p3759_Renner http://hdl.handle.net/20.500.12110/paper_00137227_v143_n10_p3759_Renner |
| _version_ |
1768543211639275520 |