The gp130 cytokines interleukin-11 and ciliary neurotropic factor regulate through specific receptors the function and growth of lactosomatotropic and folliculostellate pituitary cell lines
Two of the most potent cytokines regulating anterior pituitary cell function are leukemia inhibitory factor and interleukin-6 (IL-6), which belong to the cytokine receptor family using the common gp130 signal transducer. We studied the actions of two other members of this family, IL-11 and ciliary n...
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00137227_v141_n5_p1746_Castro http://hdl.handle.net/20.500.12110/paper_00137227_v141_n5_p1746_Castro |
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paper:paper_00137227_v141_n5_p1746_Castro2023-06-08T14:36:09Z The gp130 cytokines interleukin-11 and ciliary neurotropic factor regulate through specific receptors the function and growth of lactosomatotropic and folliculostellate pituitary cell lines Páez Pereda, Marcelo ciliary neurotrophic factor ciliary neurotrophic factor receptor cytokine glycoprotein gp 130 interleukin 11 interleukin 11 receptor leukemia inhibitory factor vasculotropin adenohypophysis alpha chain angiogenesis animal cell article cell proliferation controlled study dose response hormone synthesis hypophysis cell nonhuman priority journal rat regulatory mechanism signal transduction stellate cell Two of the most potent cytokines regulating anterior pituitary cell function are leukemia inhibitory factor and interleukin-6 (IL-6), which belong to the cytokine receptor family using the common gp130 signal transducer. We studied the actions of two other members of this family, IL-11 and ciliary neurotropic factor (CNTF), on folliculostellate (FS) cells (TtT/GF cell line) and lactosomatotropic cells (GH3 cell line). The messenger RNA (mRNA) for the α-chain specific for the IL-11 receptor (1.7 kb) and CNTF receptor (2 kb) are expressed on both cell types. In addition, we detected CNTF receptor mRNA in normal rat anterior pituitary cells. IL-11 (1.25-5 nM) dose dependently stimulated the proliferation of FS cells. CNTF, at doses from 0.4-2 nM, also significantly stimulated the growth of these cells. In addition, both cytokines significantly stimulated proliferation of lactosomatotropic GH3 cells, and CNTF stimulated hormone production (GH and PRL) at 24 h by these cells. At 16-72 h, IL-11 stimulates the secretion of the angiogenic factor vascular endothelial growth factor by FS cells. In addition, both GH3 and FS cells express CNTF mRNA. These data suggest that IL-11 and CNTF may act as growth and regulatory factors in anterior pituitary cells. Fil:Pereda, M.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2000 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00137227_v141_n5_p1746_Castro http://hdl.handle.net/20.500.12110/paper_00137227_v141_n5_p1746_Castro |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
ciliary neurotrophic factor ciliary neurotrophic factor receptor cytokine glycoprotein gp 130 interleukin 11 interleukin 11 receptor leukemia inhibitory factor vasculotropin adenohypophysis alpha chain angiogenesis animal cell article cell proliferation controlled study dose response hormone synthesis hypophysis cell nonhuman priority journal rat regulatory mechanism signal transduction stellate cell |
| spellingShingle |
ciliary neurotrophic factor ciliary neurotrophic factor receptor cytokine glycoprotein gp 130 interleukin 11 interleukin 11 receptor leukemia inhibitory factor vasculotropin adenohypophysis alpha chain angiogenesis animal cell article cell proliferation controlled study dose response hormone synthesis hypophysis cell nonhuman priority journal rat regulatory mechanism signal transduction stellate cell Páez Pereda, Marcelo The gp130 cytokines interleukin-11 and ciliary neurotropic factor regulate through specific receptors the function and growth of lactosomatotropic and folliculostellate pituitary cell lines |
| topic_facet |
ciliary neurotrophic factor ciliary neurotrophic factor receptor cytokine glycoprotein gp 130 interleukin 11 interleukin 11 receptor leukemia inhibitory factor vasculotropin adenohypophysis alpha chain angiogenesis animal cell article cell proliferation controlled study dose response hormone synthesis hypophysis cell nonhuman priority journal rat regulatory mechanism signal transduction stellate cell |
| description |
Two of the most potent cytokines regulating anterior pituitary cell function are leukemia inhibitory factor and interleukin-6 (IL-6), which belong to the cytokine receptor family using the common gp130 signal transducer. We studied the actions of two other members of this family, IL-11 and ciliary neurotropic factor (CNTF), on folliculostellate (FS) cells (TtT/GF cell line) and lactosomatotropic cells (GH3 cell line). The messenger RNA (mRNA) for the α-chain specific for the IL-11 receptor (1.7 kb) and CNTF receptor (2 kb) are expressed on both cell types. In addition, we detected CNTF receptor mRNA in normal rat anterior pituitary cells. IL-11 (1.25-5 nM) dose dependently stimulated the proliferation of FS cells. CNTF, at doses from 0.4-2 nM, also significantly stimulated the growth of these cells. In addition, both cytokines significantly stimulated proliferation of lactosomatotropic GH3 cells, and CNTF stimulated hormone production (GH and PRL) at 24 h by these cells. At 16-72 h, IL-11 stimulates the secretion of the angiogenic factor vascular endothelial growth factor by FS cells. In addition, both GH3 and FS cells express CNTF mRNA. These data suggest that IL-11 and CNTF may act as growth and regulatory factors in anterior pituitary cells. |
| author |
Páez Pereda, Marcelo |
| author_facet |
Páez Pereda, Marcelo |
| author_sort |
Páez Pereda, Marcelo |
| title |
The gp130 cytokines interleukin-11 and ciliary neurotropic factor regulate through specific receptors the function and growth of lactosomatotropic and folliculostellate pituitary cell lines |
| title_short |
The gp130 cytokines interleukin-11 and ciliary neurotropic factor regulate through specific receptors the function and growth of lactosomatotropic and folliculostellate pituitary cell lines |
| title_full |
The gp130 cytokines interleukin-11 and ciliary neurotropic factor regulate through specific receptors the function and growth of lactosomatotropic and folliculostellate pituitary cell lines |
| title_fullStr |
The gp130 cytokines interleukin-11 and ciliary neurotropic factor regulate through specific receptors the function and growth of lactosomatotropic and folliculostellate pituitary cell lines |
| title_full_unstemmed |
The gp130 cytokines interleukin-11 and ciliary neurotropic factor regulate through specific receptors the function and growth of lactosomatotropic and folliculostellate pituitary cell lines |
| title_sort |
gp130 cytokines interleukin-11 and ciliary neurotropic factor regulate through specific receptors the function and growth of lactosomatotropic and folliculostellate pituitary cell lines |
| publishDate |
2000 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00137227_v141_n5_p1746_Castro http://hdl.handle.net/20.500.12110/paper_00137227_v141_n5_p1746_Castro |
| work_keys_str_mv |
AT paezperedamarcelo thegp130cytokinesinterleukin11andciliaryneurotropicfactorregulatethroughspecificreceptorsthefunctionandgrowthoflactosomatotropicandfolliculostellatepituitarycelllines AT paezperedamarcelo gp130cytokinesinterleukin11andciliaryneurotropicfactorregulatethroughspecificreceptorsthefunctionandgrowthoflactosomatotropicandfolliculostellatepituitarycelllines |
| _version_ |
1768544209202053120 |