Effect of follicle-stimulating hormone on insulin-like growth factor-I-stimulated rat granulosa cell deoxyribonucleic acid synthesis

Granulosa cells from diethylstilbestrol-treated immature rats were cultured in a defined medium on collagen-coated plates. Thymidine incorporation was significantly increased by insulin (ED50, 656 ±110 ng/ml) and insulin-like growth factor (IGF-I; ED50, 95 ± 10 ng/ml). Insulin and IGF-I stimulations...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Bley, Miguel Andrés, Estévez, Alvaro Germán, Jiménez de Asúa, Luis A.F., Barañao, José Lino S.
Publicado:	1992
Materias:	dna follitropin somatomedin c animal cell article cell growth dna synthesis enzyme activity granulosa cell growth stimulation hormone action nonhuman ovary follicle maturation priority journal rat 1-Methyl-3-isobutylxanthine Animal Bucladesine Cell Division Cell Nucleus Cells, Cultured Cyclic AMP DNA DNA Replication Female Follicle Stimulating Hormone Granulosa Cells Insulin-Like Growth Factor I Kinetics Rats Rats, Inbred Strains Recombinant Proteins Support, Non-U.S. Gov't
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00137227_v131_n3_p1223_Bley http://hdl.handle.net/20.500.12110/paper_00137227_v131_n3_p1223_Bley
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00137227_v131_n3_p1223_Bley
record_format	dspace
spelling	paper:paper_00137227_v131_n3_p1223_Bley2023-06-08T14:36:05Z Effect of follicle-stimulating hormone on insulin-like growth factor-I-stimulated rat granulosa cell deoxyribonucleic acid synthesis Bley, Miguel Andrés Estévez, Alvaro Germán Jiménez de Asúa, Luis A.F. Barañao, José Lino S. dna follitropin somatomedin c animal cell article cell growth dna synthesis enzyme activity granulosa cell growth stimulation hormone action nonhuman ovary follicle maturation priority journal rat 1-Methyl-3-isobutylxanthine Animal Bucladesine Cell Division Cell Nucleus Cells, Cultured Cyclic AMP DNA DNA Replication Female Follicle Stimulating Hormone Granulosa Cells Insulin-Like Growth Factor I Kinetics Rats Rats, Inbred Strains Recombinant Proteins Support, Non-U.S. Gov't Granulosa cells from diethylstilbestrol-treated immature rats were cultured in a defined medium on collagen-coated plates. Thymidine incorporation was significantly increased by insulin (ED50, 656 ±110 ng/ml) and insulin-like growth factor (IGF-I; ED50, 95 ± 10 ng/ml). Insulin and IGF-I stimulations were amplified by methylisobutylxan- thine an inhibitor of phosphodiesterase activity. The effect of both peptides were also enhanced by low doses of (Bu)2cAMP (0.2-1 mM). In contrast, higher concentrations were inhibitory. Similarly, FSH produced a biphasic enhancement of the insulin- and IGF-I-stimulated DNA synthesis. Maximal effects (2- to 6-fold increases) were observed with the lower doses (2-20 ng/ml) of the gonadotropin. FSH enhancement of IGF-I-stimulated DNA synthesis was dependent on cell density. Plating densities of 3-5 X 105 cells/cm2 were required for a maximal interaction. It is concluded that FSH, acting through a cAMP-mediated pathway, may regulate granulosa cell proliferation by modulating the mitogenic effects of insulin and/or IGF-I. © 1992 by The Endocrine Society. Fil:Bley, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Estévez, A.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:De Asúa, L.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Barañao, J.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1992 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00137227_v131_n3_p1223_Bley http://hdl.handle.net/20.500.12110/paper_00137227_v131_n3_p1223_Bley
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	dna follitropin somatomedin c animal cell article cell growth dna synthesis enzyme activity granulosa cell growth stimulation hormone action nonhuman ovary follicle maturation priority journal rat 1-Methyl-3-isobutylxanthine Animal Bucladesine Cell Division Cell Nucleus Cells, Cultured Cyclic AMP DNA DNA Replication Female Follicle Stimulating Hormone Granulosa Cells Insulin-Like Growth Factor I Kinetics Rats Rats, Inbred Strains Recombinant Proteins Support, Non-U.S. Gov't
spellingShingle	dna follitropin somatomedin c animal cell article cell growth dna synthesis enzyme activity granulosa cell growth stimulation hormone action nonhuman ovary follicle maturation priority journal rat 1-Methyl-3-isobutylxanthine Animal Bucladesine Cell Division Cell Nucleus Cells, Cultured Cyclic AMP DNA DNA Replication Female Follicle Stimulating Hormone Granulosa Cells Insulin-Like Growth Factor I Kinetics Rats Rats, Inbred Strains Recombinant Proteins Support, Non-U.S. Gov't Bley, Miguel Andrés Estévez, Alvaro Germán Jiménez de Asúa, Luis A.F. Barañao, José Lino S. Effect of follicle-stimulating hormone on insulin-like growth factor-I-stimulated rat granulosa cell deoxyribonucleic acid synthesis
topic_facet	dna follitropin somatomedin c animal cell article cell growth dna synthesis enzyme activity granulosa cell growth stimulation hormone action nonhuman ovary follicle maturation priority journal rat 1-Methyl-3-isobutylxanthine Animal Bucladesine Cell Division Cell Nucleus Cells, Cultured Cyclic AMP DNA DNA Replication Female Follicle Stimulating Hormone Granulosa Cells Insulin-Like Growth Factor I Kinetics Rats Rats, Inbred Strains Recombinant Proteins Support, Non-U.S. Gov't
description	Granulosa cells from diethylstilbestrol-treated immature rats were cultured in a defined medium on collagen-coated plates. Thymidine incorporation was significantly increased by insulin (ED50, 656 ±110 ng/ml) and insulin-like growth factor (IGF-I; ED50, 95 ± 10 ng/ml). Insulin and IGF-I stimulations were amplified by methylisobutylxan- thine an inhibitor of phosphodiesterase activity. The effect of both peptides were also enhanced by low doses of (Bu)2cAMP (0.2-1 mM). In contrast, higher concentrations were inhibitory. Similarly, FSH produced a biphasic enhancement of the insulin- and IGF-I-stimulated DNA synthesis. Maximal effects (2- to 6-fold increases) were observed with the lower doses (2-20 ng/ml) of the gonadotropin. FSH enhancement of IGF-I-stimulated DNA synthesis was dependent on cell density. Plating densities of 3-5 X 105 cells/cm2 were required for a maximal interaction. It is concluded that FSH, acting through a cAMP-mediated pathway, may regulate granulosa cell proliferation by modulating the mitogenic effects of insulin and/or IGF-I. © 1992 by The Endocrine Society.
author	Bley, Miguel Andrés Estévez, Alvaro Germán Jiménez de Asúa, Luis A.F. Barañao, José Lino S.
author_facet	Bley, Miguel Andrés Estévez, Alvaro Germán Jiménez de Asúa, Luis A.F. Barañao, José Lino S.
author_sort	Bley, Miguel Andrés
title	Effect of follicle-stimulating hormone on insulin-like growth factor-I-stimulated rat granulosa cell deoxyribonucleic acid synthesis
title_short	Effect of follicle-stimulating hormone on insulin-like growth factor-I-stimulated rat granulosa cell deoxyribonucleic acid synthesis
title_full	Effect of follicle-stimulating hormone on insulin-like growth factor-I-stimulated rat granulosa cell deoxyribonucleic acid synthesis
title_fullStr	Effect of follicle-stimulating hormone on insulin-like growth factor-I-stimulated rat granulosa cell deoxyribonucleic acid synthesis
title_full_unstemmed	Effect of follicle-stimulating hormone on insulin-like growth factor-I-stimulated rat granulosa cell deoxyribonucleic acid synthesis
title_sort	effect of follicle-stimulating hormone on insulin-like growth factor-i-stimulated rat granulosa cell deoxyribonucleic acid synthesis
publishDate	1992
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00137227_v131_n3_p1223_Bley http://hdl.handle.net/20.500.12110/paper_00137227_v131_n3_p1223_Bley
work_keys_str_mv	AT bleymiguelandres effectoffolliclestimulatinghormoneoninsulinlikegrowthfactoristimulatedratgranulosacelldeoxyribonucleicacidsynthesis AT estevezalvarogerman effectoffolliclestimulatinghormoneoninsulinlikegrowthfactoristimulatedratgranulosacelldeoxyribonucleicacidsynthesis AT jimenezdeasualuisaf effectoffolliclestimulatinghormoneoninsulinlikegrowthfactoristimulatedratgranulosacelldeoxyribonucleicacidsynthesis AT baranaojoselinos effectoffolliclestimulatinghormoneoninsulinlikegrowthfactoristimulatedratgranulosacelldeoxyribonucleicacidsynthesis
_version_	1768544893963075584

Effect of follicle-stimulating hormone on insulin-like growth factor-I-stimulated rat granulosa cell deoxyribonucleic acid synthesis

Ejemplares similares