Androgen receptors in the diabetic rat
Male rats rendered diabetic by IV streptozotocin (65mg/kg body weight) were treated with exogenous insulin or testosterone. Charcoal-coated dextran and polyacrylemide gel electrophoresis techniques were employed in studying the characteristics of androgen (R1881) binding to prostate cytosol protein....
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1980
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0012186X_v18_n5_p385_Tesone http://hdl.handle.net/20.500.12110/paper_0012186X_v18_n5_p385_Tesone |
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paper:paper_0012186X_v18_n5_p385_Tesone2023-06-08T14:35:21Z Androgen receptors in the diabetic rat Tesone, Marta Calvo, Juan Carlos Barañao, José Lino S. Charreau, Eduardo Hernán Androgen receptors cytosol binding sites R1881 rat prostate streptozotocin-diabetes androgen receptor androstanolone androstanolone h 3 drug receptor insulin methyltrienolone h 3 metribolone radioisotope streptozocin testosterone unclassified drug animal experiment article diabetes mellitus endocrine system male genital system prostate rat Animals Body Weight Cytosol Diabetes Mellitus, Experimental Epididymis Estrenes Insulin, Long-Acting Male Metribolone Organ Size Prostate Rats Receptors, Androgen Receptors, Steroid Testis Testosterone Testosterone Congeners Male rats rendered diabetic by IV streptozotocin (65mg/kg body weight) were treated with exogenous insulin or testosterone. Charcoal-coated dextran and polyacrylemide gel electrophoresis techniques were employed in studying the characteristics of androgen (R1881) binding to prostate cytosol protein. In comparison with normal (N) rats, the replacement therapy of diabetic (D) animals with insulin (D+I) or testosterone (D+T) was able to restore epididymal weight (N = 0.40 ±0.04 g; D = 0.18 ± 0.02 g; D+I = 0.42 ± 0.05 g; D+T = 0.40 ± 0.06 g) and total prostate weight (N = 0.24 ± 0.02 g; D = 0.15 ± 0.02 g; D+I = 0.24 ± 0.05 g; D+T = 0.35 ± 0.06 g). Testicular endogenous content of testosterone was restored after insulin treatment (N = 154 ± 13 ng/testis; D = 41 ± 5 ng/testis; D+I = 142 ± 9 ng/testis), and significant improvements of serum testosterone levels were also achieved (N = 540 ± 64 ng/100 ml; D = 238 ± 37 ng/100 ml; D+I = 358 ± 18 ng/100 ml). Prostate cytosol of streptozotocin-diabetic rats had strongly lowered capacity for 3H-R1881 binding compared with controls (94 and 12 fmol/mg protein, respectively). Testosterone treatment produced a 3.3-fold improvement of this lowered value, whereas the increment seen with insulin was less (1.5-fold). It is emphasized that some of the improvements caused by insulin replacement therapy in diabetic animals are due to the partial restoration of testosterone secretion. Thus, the combined actions of insulin and testosterone (instead of insulin alone) seem to be of major importance in the maintenance and regulation of accessory sex glands function. © 1980 Springer-Verlag. Fil:Tesone, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Calvo, J.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Barañao, J.L.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Charreau, E.H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1980 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0012186X_v18_n5_p385_Tesone http://hdl.handle.net/20.500.12110/paper_0012186X_v18_n5_p385_Tesone |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Androgen receptors cytosol binding sites R1881 rat prostate streptozotocin-diabetes androgen receptor androstanolone androstanolone h 3 drug receptor insulin methyltrienolone h 3 metribolone radioisotope streptozocin testosterone unclassified drug animal experiment article diabetes mellitus endocrine system male genital system prostate rat Animals Body Weight Cytosol Diabetes Mellitus, Experimental Epididymis Estrenes Insulin, Long-Acting Male Metribolone Organ Size Prostate Rats Receptors, Androgen Receptors, Steroid Testis Testosterone Testosterone Congeners |
| spellingShingle |
Androgen receptors cytosol binding sites R1881 rat prostate streptozotocin-diabetes androgen receptor androstanolone androstanolone h 3 drug receptor insulin methyltrienolone h 3 metribolone radioisotope streptozocin testosterone unclassified drug animal experiment article diabetes mellitus endocrine system male genital system prostate rat Animals Body Weight Cytosol Diabetes Mellitus, Experimental Epididymis Estrenes Insulin, Long-Acting Male Metribolone Organ Size Prostate Rats Receptors, Androgen Receptors, Steroid Testis Testosterone Testosterone Congeners Tesone, Marta Calvo, Juan Carlos Barañao, José Lino S. Charreau, Eduardo Hernán Androgen receptors in the diabetic rat |
| topic_facet |
Androgen receptors cytosol binding sites R1881 rat prostate streptozotocin-diabetes androgen receptor androstanolone androstanolone h 3 drug receptor insulin methyltrienolone h 3 metribolone radioisotope streptozocin testosterone unclassified drug animal experiment article diabetes mellitus endocrine system male genital system prostate rat Animals Body Weight Cytosol Diabetes Mellitus, Experimental Epididymis Estrenes Insulin, Long-Acting Male Metribolone Organ Size Prostate Rats Receptors, Androgen Receptors, Steroid Testis Testosterone Testosterone Congeners |
| description |
Male rats rendered diabetic by IV streptozotocin (65mg/kg body weight) were treated with exogenous insulin or testosterone. Charcoal-coated dextran and polyacrylemide gel electrophoresis techniques were employed in studying the characteristics of androgen (R1881) binding to prostate cytosol protein. In comparison with normal (N) rats, the replacement therapy of diabetic (D) animals with insulin (D+I) or testosterone (D+T) was able to restore epididymal weight (N = 0.40 ±0.04 g; D = 0.18 ± 0.02 g; D+I = 0.42 ± 0.05 g; D+T = 0.40 ± 0.06 g) and total prostate weight (N = 0.24 ± 0.02 g; D = 0.15 ± 0.02 g; D+I = 0.24 ± 0.05 g; D+T = 0.35 ± 0.06 g). Testicular endogenous content of testosterone was restored after insulin treatment (N = 154 ± 13 ng/testis; D = 41 ± 5 ng/testis; D+I = 142 ± 9 ng/testis), and significant improvements of serum testosterone levels were also achieved (N = 540 ± 64 ng/100 ml; D = 238 ± 37 ng/100 ml; D+I = 358 ± 18 ng/100 ml). Prostate cytosol of streptozotocin-diabetic rats had strongly lowered capacity for 3H-R1881 binding compared with controls (94 and 12 fmol/mg protein, respectively). Testosterone treatment produced a 3.3-fold improvement of this lowered value, whereas the increment seen with insulin was less (1.5-fold). It is emphasized that some of the improvements caused by insulin replacement therapy in diabetic animals are due to the partial restoration of testosterone secretion. Thus, the combined actions of insulin and testosterone (instead of insulin alone) seem to be of major importance in the maintenance and regulation of accessory sex glands function. © 1980 Springer-Verlag. |
| author |
Tesone, Marta Calvo, Juan Carlos Barañao, José Lino S. Charreau, Eduardo Hernán |
| author_facet |
Tesone, Marta Calvo, Juan Carlos Barañao, José Lino S. Charreau, Eduardo Hernán |
| author_sort |
Tesone, Marta |
| title |
Androgen receptors in the diabetic rat |
| title_short |
Androgen receptors in the diabetic rat |
| title_full |
Androgen receptors in the diabetic rat |
| title_fullStr |
Androgen receptors in the diabetic rat |
| title_full_unstemmed |
Androgen receptors in the diabetic rat |
| title_sort |
androgen receptors in the diabetic rat |
| publishDate |
1980 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0012186X_v18_n5_p385_Tesone http://hdl.handle.net/20.500.12110/paper_0012186X_v18_n5_p385_Tesone |
| work_keys_str_mv |
AT tesonemarta androgenreceptorsinthediabeticrat AT calvojuancarlos androgenreceptorsinthediabeticrat AT baranaojoselinos androgenreceptorsinthediabeticrat AT charreaueduardohernan androgenreceptorsinthediabeticrat |
| _version_ |
1768546284285722624 |