Lipase activity sensitive to dopamine, Glucagon and cyclic amp in the Hepatopancreas of the Euryhaline burrowing crab Neohelice Granulata (Dana, 1851) (Decapoda, Grapsidae)

We studied the biochemical characteristics and modulation by dopamine, glucagon and cAMP of lipase activity in hepatopancreas of the euryhaline crab Neohelice granulata (Dana, 1851), considered to be an emergent model for biochemical, physiological and ecological research. Lipase activity was maximu...

Descripción completa

Guardado en:

Detalles Bibliográficos
Publicado:	2015
Materias:	burrowing organism catabolism crab emergence enzyme activity identification key incubation temperature effect Decapoda (Crustacea) Grapsidae
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0011216X_v88_n1_p51_LopezMananes http://hdl.handle.net/20.500.12110/paper_0011216X_v88_n1_p51_LopezMananes
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_0011216X_v88_n1_p51_LopezMananes
record_format	dspace
spelling	paper:paper_0011216X_v88_n1_p51_LopezMananes2023-06-08T14:34:48Z Lipase activity sensitive to dopamine, Glucagon and cyclic amp in the Hepatopancreas of the Euryhaline burrowing crab Neohelice Granulata (Dana, 1851) (Decapoda, Grapsidae) burrowing organism catabolism crab emergence enzyme activity identification key incubation temperature effect Decapoda (Crustacea) Grapsidae We studied the biochemical characteristics and modulation by dopamine, glucagon and cAMP of lipase activity in hepatopancreas of the euryhaline crab Neohelice granulata (Dana, 1851), considered to be an emergent model for biochemical, physiological and ecological research. Lipase activity was maximum at pH 8.5; it exhibited Michaelis-Menten kinetics (apparent Km = 0.018 mM), was highest at 37°C but appeared to be cold- and heat-tolerant, since it was high at 4°C and at 45°C. Lipase activity was enhanced upon incubation of hepatopancreas with 10?4 M dopamine (about 100%), 2 × 10?3 M glucagon (about 250%) and 10?4 M cAMP (about 150%) suggesting a role of these chemical messengers in mechanisms of regulation of lipolytic activities and its direct effect on the hepatopancreas. The concomitant decrease in triglycerides content upon dopamine and cAMP treatment suggests a link between enhanced lipase activity by these messengers and triglycerides catabolism. © 2015 Koninklijke Brill NV, Leiden. 2015 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0011216X_v88_n1_p51_LopezMananes http://hdl.handle.net/20.500.12110/paper_0011216X_v88_n1_p51_LopezMananes
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	burrowing organism catabolism crab emergence enzyme activity identification key incubation temperature effect Decapoda (Crustacea) Grapsidae
spellingShingle	burrowing organism catabolism crab emergence enzyme activity identification key incubation temperature effect Decapoda (Crustacea) Grapsidae Lipase activity sensitive to dopamine, Glucagon and cyclic amp in the Hepatopancreas of the Euryhaline burrowing crab Neohelice Granulata (Dana, 1851) (Decapoda, Grapsidae)
topic_facet	burrowing organism catabolism crab emergence enzyme activity identification key incubation temperature effect Decapoda (Crustacea) Grapsidae
description	We studied the biochemical characteristics and modulation by dopamine, glucagon and cAMP of lipase activity in hepatopancreas of the euryhaline crab Neohelice granulata (Dana, 1851), considered to be an emergent model for biochemical, physiological and ecological research. Lipase activity was maximum at pH 8.5; it exhibited Michaelis-Menten kinetics (apparent Km = 0.018 mM), was highest at 37°C but appeared to be cold- and heat-tolerant, since it was high at 4°C and at 45°C. Lipase activity was enhanced upon incubation of hepatopancreas with 10?4 M dopamine (about 100%), 2 × 10?3 M glucagon (about 250%) and 10?4 M cAMP (about 150%) suggesting a role of these chemical messengers in mechanisms of regulation of lipolytic activities and its direct effect on the hepatopancreas. The concomitant decrease in triglycerides content upon dopamine and cAMP treatment suggests a link between enhanced lipase activity by these messengers and triglycerides catabolism. © 2015 Koninklijke Brill NV, Leiden.
title	Lipase activity sensitive to dopamine, Glucagon and cyclic amp in the Hepatopancreas of the Euryhaline burrowing crab Neohelice Granulata (Dana, 1851) (Decapoda, Grapsidae)
title_short	Lipase activity sensitive to dopamine, Glucagon and cyclic amp in the Hepatopancreas of the Euryhaline burrowing crab Neohelice Granulata (Dana, 1851) (Decapoda, Grapsidae)
title_full	Lipase activity sensitive to dopamine, Glucagon and cyclic amp in the Hepatopancreas of the Euryhaline burrowing crab Neohelice Granulata (Dana, 1851) (Decapoda, Grapsidae)
title_fullStr	Lipase activity sensitive to dopamine, Glucagon and cyclic amp in the Hepatopancreas of the Euryhaline burrowing crab Neohelice Granulata (Dana, 1851) (Decapoda, Grapsidae)
title_full_unstemmed	Lipase activity sensitive to dopamine, Glucagon and cyclic amp in the Hepatopancreas of the Euryhaline burrowing crab Neohelice Granulata (Dana, 1851) (Decapoda, Grapsidae)
title_sort	lipase activity sensitive to dopamine, glucagon and cyclic amp in the hepatopancreas of the euryhaline burrowing crab neohelice granulata (dana, 1851) (decapoda, grapsidae)
publishDate	2015
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0011216X_v88_n1_p51_LopezMananes http://hdl.handle.net/20.500.12110/paper_0011216X_v88_n1_p51_LopezMananes
_version_	1768543877528027136

Lipase activity sensitive to dopamine, Glucagon and cyclic amp in the Hepatopancreas of the Euryhaline burrowing crab Neohelice Granulata (Dana, 1851) (Decapoda, Grapsidae)

Ejemplares similares