Decreased Tamm-Horsfall protein in lithiasic patients
Objectives: Tamm-Horsfall glycoprotein (THP) is the most abundant substance of renal origin appearing in urine. It seems to be one of the major inhibitors of calcium oxalate crystal nucleation and/or aggregation, but its role in the pathogenesis of stone formation has not yet been clarified. The pre...
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1997
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00099120_v30_n1_p63_Romero http://hdl.handle.net/20.500.12110/paper_00099120_v30_n1_p63_Romero |
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paper:paper_00099120_v30_n1_p63_Romero2023-06-08T14:34:09Z Decreased Tamm-Horsfall protein in lithiasic patients Nesse, Alcira Beatriz ELISA lithiasic patients stone formers Tamm-Horsfall glycoprotein urolithiasis uromodulin tamm horsfall glycoprotein article enzyme linked immunosorbent assay human priority journal protein determination protein urine level risk assessment stone formation urolithiasis Adult Aged Animals Enzyme-Linked Immunosorbent Assay Female Humans Kidney Calculi Male Middle Aged Mucoproteins Rabbits Objectives: Tamm-Horsfall glycoprotein (THP) is the most abundant substance of renal origin appearing in urine. It seems to be one of the major inhibitors of calcium oxalate crystal nucleation and/or aggregation, but its role in the pathogenesis of stone formation has not yet been clarified. The present work was undertaken to quantify THP excretion in lithiasic (L) patients, so as to determine if these levels are different from those of control subjects (C). Design and Methods: THP was isolated from human urine by reprecipitation steps, rabbit antiTHP antibody was obtained and its specificity determined, an ELISA was developed and technical conditions standardized, and quantitative measurements of urinary THP were performed on samples from L patients, who had suffered more than one lithiasic episode, and from C subjects. Microtiter plates coated with THP or diluted urine samples were subjected to successive incubation with antiTHP and alkaline phosphatase antirabbit IgG. Results: A good correlation between measured absorbance and THP concentration in standard and urine samples was observed. Data, expressed as Median and Interquartile Range, are 388/209626 μg THP/mmol creatinine for C (n = 85) and 124/82-171 μg THP/mmol creatinine for L (n = 23). Conclusions: We have obtained an antiserum antiTHP that can be used in the ELISA technique to determine reliable urinary THP values. The results show a significant decrease of THP excretion in recurrent stone formers compared to controls (p < 0.001) and may have interesting implications in the pathogenesis of urolithiasis. Fil:Nesse, A.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1997 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00099120_v30_n1_p63_Romero http://hdl.handle.net/20.500.12110/paper_00099120_v30_n1_p63_Romero |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
ELISA lithiasic patients stone formers Tamm-Horsfall glycoprotein urolithiasis uromodulin tamm horsfall glycoprotein article enzyme linked immunosorbent assay human priority journal protein determination protein urine level risk assessment stone formation urolithiasis Adult Aged Animals Enzyme-Linked Immunosorbent Assay Female Humans Kidney Calculi Male Middle Aged Mucoproteins Rabbits |
| spellingShingle |
ELISA lithiasic patients stone formers Tamm-Horsfall glycoprotein urolithiasis uromodulin tamm horsfall glycoprotein article enzyme linked immunosorbent assay human priority journal protein determination protein urine level risk assessment stone formation urolithiasis Adult Aged Animals Enzyme-Linked Immunosorbent Assay Female Humans Kidney Calculi Male Middle Aged Mucoproteins Rabbits Nesse, Alcira Beatriz Decreased Tamm-Horsfall protein in lithiasic patients |
| topic_facet |
ELISA lithiasic patients stone formers Tamm-Horsfall glycoprotein urolithiasis uromodulin tamm horsfall glycoprotein article enzyme linked immunosorbent assay human priority journal protein determination protein urine level risk assessment stone formation urolithiasis Adult Aged Animals Enzyme-Linked Immunosorbent Assay Female Humans Kidney Calculi Male Middle Aged Mucoproteins Rabbits |
| description |
Objectives: Tamm-Horsfall glycoprotein (THP) is the most abundant substance of renal origin appearing in urine. It seems to be one of the major inhibitors of calcium oxalate crystal nucleation and/or aggregation, but its role in the pathogenesis of stone formation has not yet been clarified. The present work was undertaken to quantify THP excretion in lithiasic (L) patients, so as to determine if these levels are different from those of control subjects (C). Design and Methods: THP was isolated from human urine by reprecipitation steps, rabbit antiTHP antibody was obtained and its specificity determined, an ELISA was developed and technical conditions standardized, and quantitative measurements of urinary THP were performed on samples from L patients, who had suffered more than one lithiasic episode, and from C subjects. Microtiter plates coated with THP or diluted urine samples were subjected to successive incubation with antiTHP and alkaline phosphatase antirabbit IgG. Results: A good correlation between measured absorbance and THP concentration in standard and urine samples was observed. Data, expressed as Median and Interquartile Range, are 388/209626 μg THP/mmol creatinine for C (n = 85) and 124/82-171 μg THP/mmol creatinine for L (n = 23). Conclusions: We have obtained an antiserum antiTHP that can be used in the ELISA technique to determine reliable urinary THP values. The results show a significant decrease of THP excretion in recurrent stone formers compared to controls (p < 0.001) and may have interesting implications in the pathogenesis of urolithiasis. |
| author |
Nesse, Alcira Beatriz |
| author_facet |
Nesse, Alcira Beatriz |
| author_sort |
Nesse, Alcira Beatriz |
| title |
Decreased Tamm-Horsfall protein in lithiasic patients |
| title_short |
Decreased Tamm-Horsfall protein in lithiasic patients |
| title_full |
Decreased Tamm-Horsfall protein in lithiasic patients |
| title_fullStr |
Decreased Tamm-Horsfall protein in lithiasic patients |
| title_full_unstemmed |
Decreased Tamm-Horsfall protein in lithiasic patients |
| title_sort |
decreased tamm-horsfall protein in lithiasic patients |
| publishDate |
1997 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00099120_v30_n1_p63_Romero http://hdl.handle.net/20.500.12110/paper_00099120_v30_n1_p63_Romero |
| work_keys_str_mv |
AT nessealcirabeatriz decreasedtammhorsfallproteininlithiasicpatients |
| _version_ |
1768542249199599616 |