Comparison of the determination of superoxide dismutase and antioxidant capacity in neurological patients using two different procedures
As oxidative stress in relation with neurological diseases has become an important point in recent research, simple methods to be used in epidemiological studies and clinical practice are required. The hypothesis that the analytical methods used in research laboratories (RLM) can be used interchange...
Guardado en:
| Autor principal: | |
|---|---|
| Publicado: |
2000
|
| Materias: | |
| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00098981_v301_n1-2_p87_Serra http://hdl.handle.net/20.500.12110/paper_00098981_v301_n1-2_p87_Serra |
| Aporte de: |
| id |
paper:paper_00098981_v301_n1-2_p87_Serra |
|---|---|
| record_format |
dspace |
| spelling |
paper:paper_00098981_v301_n1-2_p87_Serra2023-06-08T14:34:05Z Comparison of the determination of superoxide dismutase and antioxidant capacity in neurological patients using two different procedures Marschoff, Enrique R. Analytical methods Antioxidant capacity Neurological diseases Oxidative stress Superoxide dismutase antioxidant superoxide dismutase Alzheimer disease analytic method article clinical practice controlled study correlation function disease marker female human major clinical study male medical research multiinfarct dementia neurologic disease oxidative stress Parkinson disease patient selection priority journal Alzheimer Disease Antioxidants Case-Control Studies Comparative Study Dementia, Vascular Human Parkinson Disease Superoxide Dismutase Support, Non-U.S. Gov't As oxidative stress in relation with neurological diseases has become an important point in recent research, simple methods to be used in epidemiological studies and clinical practice are required. The hypothesis that the analytical methods used in research laboratories (RLM) can be used interchangeably with commercial kits (CKM) for SOD and TRAP is tested. Both methods were compared using linear transformations of the RLM measurements into the CKM scales. Data were obtained from Alzheimer's, Parkinson's, and vascular dementia patients and controls. The lack of fit and the run's test of residuals were not significant, but the same sign method detected significant nonlinearities (P < 0.000001 for SOD, P < 0.01 for TRAP). The intragroup CVs of both methods were comparable for TRAP, while in the RLM determinations of SOD resulted in <50% of those obtained with the CKM. The ANCOVA comparison of the regression parameters across the clinical groups resulted significant for SOD (P < 0.0001) and not significant for TRAP. Both methods agree in describing the features of the clinical groups, but the degree of agreement at the individual concentration was poor and they could not be readily intercalibrated. Normal and pathological values should be obtained independently for the CKM to insure their applicability to large populations. (C) 2000 Elsevier Science B.V. Fil:Marschoff, E.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2000 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00098981_v301_n1-2_p87_Serra http://hdl.handle.net/20.500.12110/paper_00098981_v301_n1-2_p87_Serra |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Analytical methods Antioxidant capacity Neurological diseases Oxidative stress Superoxide dismutase antioxidant superoxide dismutase Alzheimer disease analytic method article clinical practice controlled study correlation function disease marker female human major clinical study male medical research multiinfarct dementia neurologic disease oxidative stress Parkinson disease patient selection priority journal Alzheimer Disease Antioxidants Case-Control Studies Comparative Study Dementia, Vascular Human Parkinson Disease Superoxide Dismutase Support, Non-U.S. Gov't |
| spellingShingle |
Analytical methods Antioxidant capacity Neurological diseases Oxidative stress Superoxide dismutase antioxidant superoxide dismutase Alzheimer disease analytic method article clinical practice controlled study correlation function disease marker female human major clinical study male medical research multiinfarct dementia neurologic disease oxidative stress Parkinson disease patient selection priority journal Alzheimer Disease Antioxidants Case-Control Studies Comparative Study Dementia, Vascular Human Parkinson Disease Superoxide Dismutase Support, Non-U.S. Gov't Marschoff, Enrique R. Comparison of the determination of superoxide dismutase and antioxidant capacity in neurological patients using two different procedures |
| topic_facet |
Analytical methods Antioxidant capacity Neurological diseases Oxidative stress Superoxide dismutase antioxidant superoxide dismutase Alzheimer disease analytic method article clinical practice controlled study correlation function disease marker female human major clinical study male medical research multiinfarct dementia neurologic disease oxidative stress Parkinson disease patient selection priority journal Alzheimer Disease Antioxidants Case-Control Studies Comparative Study Dementia, Vascular Human Parkinson Disease Superoxide Dismutase Support, Non-U.S. Gov't |
| description |
As oxidative stress in relation with neurological diseases has become an important point in recent research, simple methods to be used in epidemiological studies and clinical practice are required. The hypothesis that the analytical methods used in research laboratories (RLM) can be used interchangeably with commercial kits (CKM) for SOD and TRAP is tested. Both methods were compared using linear transformations of the RLM measurements into the CKM scales. Data were obtained from Alzheimer's, Parkinson's, and vascular dementia patients and controls. The lack of fit and the run's test of residuals were not significant, but the same sign method detected significant nonlinearities (P < 0.000001 for SOD, P < 0.01 for TRAP). The intragroup CVs of both methods were comparable for TRAP, while in the RLM determinations of SOD resulted in <50% of those obtained with the CKM. The ANCOVA comparison of the regression parameters across the clinical groups resulted significant for SOD (P < 0.0001) and not significant for TRAP. Both methods agree in describing the features of the clinical groups, but the degree of agreement at the individual concentration was poor and they could not be readily intercalibrated. Normal and pathological values should be obtained independently for the CKM to insure their applicability to large populations. (C) 2000 Elsevier Science B.V. |
| author |
Marschoff, Enrique R. |
| author_facet |
Marschoff, Enrique R. |
| author_sort |
Marschoff, Enrique R. |
| title |
Comparison of the determination of superoxide dismutase and antioxidant capacity in neurological patients using two different procedures |
| title_short |
Comparison of the determination of superoxide dismutase and antioxidant capacity in neurological patients using two different procedures |
| title_full |
Comparison of the determination of superoxide dismutase and antioxidant capacity in neurological patients using two different procedures |
| title_fullStr |
Comparison of the determination of superoxide dismutase and antioxidant capacity in neurological patients using two different procedures |
| title_full_unstemmed |
Comparison of the determination of superoxide dismutase and antioxidant capacity in neurological patients using two different procedures |
| title_sort |
comparison of the determination of superoxide dismutase and antioxidant capacity in neurological patients using two different procedures |
| publishDate |
2000 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00098981_v301_n1-2_p87_Serra http://hdl.handle.net/20.500.12110/paper_00098981_v301_n1-2_p87_Serra |
| work_keys_str_mv |
AT marschoffenriquer comparisonofthedeterminationofsuperoxidedismutaseandantioxidantcapacityinneurologicalpatientsusingtwodifferentprocedures |
| _version_ |
1768544667867021312 |