Anti-adenovirus activity of epiandrosterone and dehydroepiandrosterone derivatives
Background: Dehydroepiandrosterone (DHEA) exhibits a wide range of biological functions including antiviral activity. In this work, we present in vitro anti-adenovirus (AdV) activity of seven DHEA and twelve epiandrosterone (EA) analogues. Methods: The cytotoxic effect of the compounds was determine...
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paper:paper_00093157_v56_n2_p158_Romanutti2023-06-08T14:34:00Z Anti-adenovirus activity of epiandrosterone and dehydroepiandrosterone derivatives Romanutti, Carina Bruttomesso, Andrea Claudia Castilla, Viviana Wachsman, Mónica Beatriz Adenovirus Anti-adenovirus activity Antiviral activity Dehydroepiandrosterone analogues DHEA 16alpha hydroxyprasterone 17 oxo 5alpha androstan 3beta yl acetate 17 oxo 5alpha androstan 3beta,16alpha diyl diacetate 17 oxo 5alpha androstan 3beta,16beta diyl diacetate 17 oxoandrost 5 en 3beta yl acetate 17 oxoandrost 5 en 3beta,16alpha diyl diacetate 17,17 ethylendioxy 5alpha androst 15 en 3beta ol 17,17 ethylendioxy 5alpha androstan 16alpha bromo 3beta ol 17,17 ethylendioxy 5alpha androstan 3beta ol 17,17 ethylendioxy 5alpha androstan 3beta yl acetate 17,17 ethylendioxyandrost 5 en 3beta ol 21 norpregna 5,17(20) dien 3beta,16alpha diyl diacetate 3beta hydroxy 5alpha androst 15 en 17 one 3beta,15alpha dihydroxy 5alpha androstan 17 one 3beta,15beta dihydroxy 5alpha androstan 17 one 3beta,16alpha dihydroxy 5alpha androstan 17 one 3beta,6alpha dihydroxy 5alpha androstan 17 one androsta 5,16 dien 3beta,17 diyl diacetate antivirus agent cidofovir epiandrosterone prasterone unclassified drug virus protein Adenovirus antiviral activity article controlled study drug cytotoxicity drug mechanism drug selectivity drug structure human human cell in vitro study priority journal protein synthesis regulation virus attachment virus entry virus inhibition virus particle virus replication Western blotting Adenoviridae Androsterone Animals Antiviral Agents Blotting, Western Cercopithecus aethiops Cytosine Dehydroepiandrosterone Humans Mice Phosphonic Acids Vero Cells Viral Proteins Virus Replication Background: Dehydroepiandrosterone (DHEA) exhibits a wide range of biological functions including antiviral activity. In this work, we present in vitro anti-adenovirus (AdV) activity of seven DHEA and twelve epiandrosterone (EA) analogues. Methods: The cytotoxic effect of the compounds was determined by the MTT assay and the antiviral activity by a virus yield inhibition assay. The mode of antiviral activity was examined using time-of-addition experiments, adsorption and internalization assays and Western blot analysis. Results: EA, DHEA, and two synthetic derivatives inhibit virus replication with selectivity indices ranging between 42 and 83. Virus adsorption and internalization are not the target of the inhibitory action; meanwhile, AdV protein synthesis was diminished in the presence of DHEA. Conclusions: DHEA and some synthetic derivatives present antiviral activity similar to cidofovir, which was used as reference drug. These steroidal compounds adversely affect virus protein synthesis and viral mature particle formation. Copyright © 2010 S. Karger AG, Basel. Fil:Romanutti, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bruttomesso, A.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Castilla, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Wachsman, M.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2010 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00093157_v56_n2_p158_Romanutti http://hdl.handle.net/20.500.12110/paper_00093157_v56_n2_p158_Romanutti |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Adenovirus Anti-adenovirus activity Antiviral activity Dehydroepiandrosterone analogues DHEA 16alpha hydroxyprasterone 17 oxo 5alpha androstan 3beta yl acetate 17 oxo 5alpha androstan 3beta,16alpha diyl diacetate 17 oxo 5alpha androstan 3beta,16beta diyl diacetate 17 oxoandrost 5 en 3beta yl acetate 17 oxoandrost 5 en 3beta,16alpha diyl diacetate 17,17 ethylendioxy 5alpha androst 15 en 3beta ol 17,17 ethylendioxy 5alpha androstan 16alpha bromo 3beta ol 17,17 ethylendioxy 5alpha androstan 3beta ol 17,17 ethylendioxy 5alpha androstan 3beta yl acetate 17,17 ethylendioxyandrost 5 en 3beta ol 21 norpregna 5,17(20) dien 3beta,16alpha diyl diacetate 3beta hydroxy 5alpha androst 15 en 17 one 3beta,15alpha dihydroxy 5alpha androstan 17 one 3beta,15beta dihydroxy 5alpha androstan 17 one 3beta,16alpha dihydroxy 5alpha androstan 17 one 3beta,6alpha dihydroxy 5alpha androstan 17 one androsta 5,16 dien 3beta,17 diyl diacetate antivirus agent cidofovir epiandrosterone prasterone unclassified drug virus protein Adenovirus antiviral activity article controlled study drug cytotoxicity drug mechanism drug selectivity drug structure human human cell in vitro study priority journal protein synthesis regulation virus attachment virus entry virus inhibition virus particle virus replication Western blotting Adenoviridae Androsterone Animals Antiviral Agents Blotting, Western Cercopithecus aethiops Cytosine Dehydroepiandrosterone Humans Mice Phosphonic Acids Vero Cells Viral Proteins Virus Replication |
| spellingShingle |
Adenovirus Anti-adenovirus activity Antiviral activity Dehydroepiandrosterone analogues DHEA 16alpha hydroxyprasterone 17 oxo 5alpha androstan 3beta yl acetate 17 oxo 5alpha androstan 3beta,16alpha diyl diacetate 17 oxo 5alpha androstan 3beta,16beta diyl diacetate 17 oxoandrost 5 en 3beta yl acetate 17 oxoandrost 5 en 3beta,16alpha diyl diacetate 17,17 ethylendioxy 5alpha androst 15 en 3beta ol 17,17 ethylendioxy 5alpha androstan 16alpha bromo 3beta ol 17,17 ethylendioxy 5alpha androstan 3beta ol 17,17 ethylendioxy 5alpha androstan 3beta yl acetate 17,17 ethylendioxyandrost 5 en 3beta ol 21 norpregna 5,17(20) dien 3beta,16alpha diyl diacetate 3beta hydroxy 5alpha androst 15 en 17 one 3beta,15alpha dihydroxy 5alpha androstan 17 one 3beta,15beta dihydroxy 5alpha androstan 17 one 3beta,16alpha dihydroxy 5alpha androstan 17 one 3beta,6alpha dihydroxy 5alpha androstan 17 one androsta 5,16 dien 3beta,17 diyl diacetate antivirus agent cidofovir epiandrosterone prasterone unclassified drug virus protein Adenovirus antiviral activity article controlled study drug cytotoxicity drug mechanism drug selectivity drug structure human human cell in vitro study priority journal protein synthesis regulation virus attachment virus entry virus inhibition virus particle virus replication Western blotting Adenoviridae Androsterone Animals Antiviral Agents Blotting, Western Cercopithecus aethiops Cytosine Dehydroepiandrosterone Humans Mice Phosphonic Acids Vero Cells Viral Proteins Virus Replication Romanutti, Carina Bruttomesso, Andrea Claudia Castilla, Viviana Wachsman, Mónica Beatriz Anti-adenovirus activity of epiandrosterone and dehydroepiandrosterone derivatives |
| topic_facet |
Adenovirus Anti-adenovirus activity Antiviral activity Dehydroepiandrosterone analogues DHEA 16alpha hydroxyprasterone 17 oxo 5alpha androstan 3beta yl acetate 17 oxo 5alpha androstan 3beta,16alpha diyl diacetate 17 oxo 5alpha androstan 3beta,16beta diyl diacetate 17 oxoandrost 5 en 3beta yl acetate 17 oxoandrost 5 en 3beta,16alpha diyl diacetate 17,17 ethylendioxy 5alpha androst 15 en 3beta ol 17,17 ethylendioxy 5alpha androstan 16alpha bromo 3beta ol 17,17 ethylendioxy 5alpha androstan 3beta ol 17,17 ethylendioxy 5alpha androstan 3beta yl acetate 17,17 ethylendioxyandrost 5 en 3beta ol 21 norpregna 5,17(20) dien 3beta,16alpha diyl diacetate 3beta hydroxy 5alpha androst 15 en 17 one 3beta,15alpha dihydroxy 5alpha androstan 17 one 3beta,15beta dihydroxy 5alpha androstan 17 one 3beta,16alpha dihydroxy 5alpha androstan 17 one 3beta,6alpha dihydroxy 5alpha androstan 17 one androsta 5,16 dien 3beta,17 diyl diacetate antivirus agent cidofovir epiandrosterone prasterone unclassified drug virus protein Adenovirus antiviral activity article controlled study drug cytotoxicity drug mechanism drug selectivity drug structure human human cell in vitro study priority journal protein synthesis regulation virus attachment virus entry virus inhibition virus particle virus replication Western blotting Adenoviridae Androsterone Animals Antiviral Agents Blotting, Western Cercopithecus aethiops Cytosine Dehydroepiandrosterone Humans Mice Phosphonic Acids Vero Cells Viral Proteins Virus Replication |
| description |
Background: Dehydroepiandrosterone (DHEA) exhibits a wide range of biological functions including antiviral activity. In this work, we present in vitro anti-adenovirus (AdV) activity of seven DHEA and twelve epiandrosterone (EA) analogues. Methods: The cytotoxic effect of the compounds was determined by the MTT assay and the antiviral activity by a virus yield inhibition assay. The mode of antiviral activity was examined using time-of-addition experiments, adsorption and internalization assays and Western blot analysis. Results: EA, DHEA, and two synthetic derivatives inhibit virus replication with selectivity indices ranging between 42 and 83. Virus adsorption and internalization are not the target of the inhibitory action; meanwhile, AdV protein synthesis was diminished in the presence of DHEA. Conclusions: DHEA and some synthetic derivatives present antiviral activity similar to cidofovir, which was used as reference drug. These steroidal compounds adversely affect virus protein synthesis and viral mature particle formation. Copyright © 2010 S. Karger AG, Basel. |
| author |
Romanutti, Carina Bruttomesso, Andrea Claudia Castilla, Viviana Wachsman, Mónica Beatriz |
| author_facet |
Romanutti, Carina Bruttomesso, Andrea Claudia Castilla, Viviana Wachsman, Mónica Beatriz |
| author_sort |
Romanutti, Carina |
| title |
Anti-adenovirus activity of epiandrosterone and dehydroepiandrosterone derivatives |
| title_short |
Anti-adenovirus activity of epiandrosterone and dehydroepiandrosterone derivatives |
| title_full |
Anti-adenovirus activity of epiandrosterone and dehydroepiandrosterone derivatives |
| title_fullStr |
Anti-adenovirus activity of epiandrosterone and dehydroepiandrosterone derivatives |
| title_full_unstemmed |
Anti-adenovirus activity of epiandrosterone and dehydroepiandrosterone derivatives |
| title_sort |
anti-adenovirus activity of epiandrosterone and dehydroepiandrosterone derivatives |
| publishDate |
2010 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00093157_v56_n2_p158_Romanutti http://hdl.handle.net/20.500.12110/paper_00093157_v56_n2_p158_Romanutti |
| work_keys_str_mv |
AT romanutticarina antiadenovirusactivityofepiandrosteroneanddehydroepiandrosteronederivatives AT bruttomessoandreaclaudia antiadenovirusactivityofepiandrosteroneanddehydroepiandrosteronederivatives AT castillaviviana antiadenovirusactivityofepiandrosteroneanddehydroepiandrosteronederivatives AT wachsmanmonicabeatriz antiadenovirusactivityofepiandrosteroneanddehydroepiandrosteronederivatives |
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1768545170295357440 |