Herpes simplex virus-inhibitory sulfated xylogalactans from the red seaweed Nothogenia fastigiata
Two sulfated xylogalactans (F1 and F7), isolated from the red seaweed Nothogenia fastigiata, achieved a dose-dependent inhibition of the replication of herpes simplex virus type 1 (HSV-1) in Vero cells, with 50% effective doses in the range of 15.0-32.6 μg/ml, and without affecting cell viability at...
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1996
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00093157_v42_n1_p57_Damonte http://hdl.handle.net/20.500.12110/paper_00093157_v42_n1_p57_Damonte |
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paper:paper_00093157_v42_n1_p57_Damonte2023-06-08T14:33:59Z Herpes simplex virus-inhibitory sulfated xylogalactans from the red seaweed Nothogenia fastigiata Damonte, Elsa Beatriz Matulewicz, María Cristina Cerezo, Alberto Saúl Coto, Celia Esther Antiviral properties Herpesviruses Red seaweed Sulfated xylogalactans antivirus agent galactan polysaccharide sulfate antiviral activity article cell culture cell viability controlled study dose response herpes simplex virus herpes simplex virus 1 host cell internalization nonhuman priority journal seaweed vero cell virus adsorption virus replication Animal Antiviral Agents Cercopithecus aethiops Galactans Herpesvirus 1, Human Seaweed Support, Non-U.S. Gov't Vero Cells Two sulfated xylogalactans (F1 and F7), isolated from the red seaweed Nothogenia fastigiata, achieved a dose-dependent inhibition of the replication of herpes simplex virus type 1 (HSV-1) in Vero cells, with 50% effective doses in the range of 15.0-32.6 μg/ml, and without affecting cell viability at concentrations up to 200 μg/ml. The presence of sulfate groups in the molecule was essential for the antiviral properties of these polysaccharides. F7 afforded significant inhibition in HSV-1 yield if added to the cell cultures simultaneously with virus inoculum, but had no effect when it was added after I h of infection. Analysis of the early events of the viral replicative cycle showed that the anti-HSV effect of F7 was due to a specific inhibition of virus attachment to the host cell, whereas virus internalization was not impaired. Fil:Damonte, E.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Matulewicz, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cerezo, A.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Coto, C.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1996 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00093157_v42_n1_p57_Damonte http://hdl.handle.net/20.500.12110/paper_00093157_v42_n1_p57_Damonte |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Antiviral properties Herpesviruses Red seaweed Sulfated xylogalactans antivirus agent galactan polysaccharide sulfate antiviral activity article cell culture cell viability controlled study dose response herpes simplex virus herpes simplex virus 1 host cell internalization nonhuman priority journal seaweed vero cell virus adsorption virus replication Animal Antiviral Agents Cercopithecus aethiops Galactans Herpesvirus 1, Human Seaweed Support, Non-U.S. Gov't Vero Cells |
| spellingShingle |
Antiviral properties Herpesviruses Red seaweed Sulfated xylogalactans antivirus agent galactan polysaccharide sulfate antiviral activity article cell culture cell viability controlled study dose response herpes simplex virus herpes simplex virus 1 host cell internalization nonhuman priority journal seaweed vero cell virus adsorption virus replication Animal Antiviral Agents Cercopithecus aethiops Galactans Herpesvirus 1, Human Seaweed Support, Non-U.S. Gov't Vero Cells Damonte, Elsa Beatriz Matulewicz, María Cristina Cerezo, Alberto Saúl Coto, Celia Esther Herpes simplex virus-inhibitory sulfated xylogalactans from the red seaweed Nothogenia fastigiata |
| topic_facet |
Antiviral properties Herpesviruses Red seaweed Sulfated xylogalactans antivirus agent galactan polysaccharide sulfate antiviral activity article cell culture cell viability controlled study dose response herpes simplex virus herpes simplex virus 1 host cell internalization nonhuman priority journal seaweed vero cell virus adsorption virus replication Animal Antiviral Agents Cercopithecus aethiops Galactans Herpesvirus 1, Human Seaweed Support, Non-U.S. Gov't Vero Cells |
| description |
Two sulfated xylogalactans (F1 and F7), isolated from the red seaweed Nothogenia fastigiata, achieved a dose-dependent inhibition of the replication of herpes simplex virus type 1 (HSV-1) in Vero cells, with 50% effective doses in the range of 15.0-32.6 μg/ml, and without affecting cell viability at concentrations up to 200 μg/ml. The presence of sulfate groups in the molecule was essential for the antiviral properties of these polysaccharides. F7 afforded significant inhibition in HSV-1 yield if added to the cell cultures simultaneously with virus inoculum, but had no effect when it was added after I h of infection. Analysis of the early events of the viral replicative cycle showed that the anti-HSV effect of F7 was due to a specific inhibition of virus attachment to the host cell, whereas virus internalization was not impaired. |
| author |
Damonte, Elsa Beatriz Matulewicz, María Cristina Cerezo, Alberto Saúl Coto, Celia Esther |
| author_facet |
Damonte, Elsa Beatriz Matulewicz, María Cristina Cerezo, Alberto Saúl Coto, Celia Esther |
| author_sort |
Damonte, Elsa Beatriz |
| title |
Herpes simplex virus-inhibitory sulfated xylogalactans from the red seaweed Nothogenia fastigiata |
| title_short |
Herpes simplex virus-inhibitory sulfated xylogalactans from the red seaweed Nothogenia fastigiata |
| title_full |
Herpes simplex virus-inhibitory sulfated xylogalactans from the red seaweed Nothogenia fastigiata |
| title_fullStr |
Herpes simplex virus-inhibitory sulfated xylogalactans from the red seaweed Nothogenia fastigiata |
| title_full_unstemmed |
Herpes simplex virus-inhibitory sulfated xylogalactans from the red seaweed Nothogenia fastigiata |
| title_sort |
herpes simplex virus-inhibitory sulfated xylogalactans from the red seaweed nothogenia fastigiata |
| publishDate |
1996 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00093157_v42_n1_p57_Damonte http://hdl.handle.net/20.500.12110/paper_00093157_v42_n1_p57_Damonte |
| work_keys_str_mv |
AT damonteelsabeatriz herpessimplexvirusinhibitorysulfatedxylogalactansfromtheredseaweednothogeniafastigiata AT matulewiczmariacristina herpessimplexvirusinhibitorysulfatedxylogalactansfromtheredseaweednothogeniafastigiata AT cerezoalbertosaul herpessimplexvirusinhibitorysulfatedxylogalactansfromtheredseaweednothogeniafastigiata AT cotoceliaesther herpessimplexvirusinhibitorysulfatedxylogalactansfromtheredseaweednothogeniafastigiata |
| _version_ |
1768546521178963968 |