Studies on the cationization of agarose

Cationized agaroses with different degrees of substitution (0.04-0.77) were synthesized, employing 3-chloro-2-hydroxypropyltrimethylammonium chloride (CHPTAC). The influence of different reaction parameters on the substitution degree and molecular weight was evaluated. The investigated parameters we...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Prado, Héctor Juan, Matulewicz, María Cristina, Bonelli, Pablo R., Cukierman, Ana Lea
Publicado:	2011
Materias:	3-Chloro-2- hydroxypropyltrimethylammonium chloride Agarose Cationic polysaccharide Cationization 3-chloro-2-hydroxypropyltrimethylammonium chloride Alkoxides Cationized Degrees of substitution Electrospray ionization mass spectrometry Higher-degree Large-scale applications Maximum degree Methanolysis NMR spectroscopy Optimum concentration Reaction parameters Reaction time Scanning electronic microscopy Substitution degree Viscosimetry Chlorine compounds Electrospray ionization Infrared spectroscopy Mass spectrometry Molecular weight Nuclear magnetic resonance spectroscopy Scales (weighing instruments) agarose epoxide galactose polysaccharide article carbon nuclear magnetic resonance chemical structure electrospray mass spectrometry infrared spectroscopy methanolysis priority journal proton nuclear magnetic resonance scanning electron microscopy substitution reaction viscometry Borohydrides Cations Infrared Rays Magnetic Resonance Spectroscopy Microscopy, Electron, Scanning Propanols Quaternary Ammonium Compounds Sepharose Spectrometry, Mass, Electrospray Ionization Spectrophotometry, Infrared Viscosity
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00086215_v346_n2_p311_Prado http://hdl.handle.net/20.500.12110/paper_00086215_v346_n2_p311_Prado
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00086215_v346_n2_p311_Prado
record_format	dspace
spelling	paper:paper_00086215_v346_n2_p311_Prado2023-06-08T14:33:00Z Studies on the cationization of agarose Prado, Héctor Juan Matulewicz, María Cristina Bonelli, Pablo R. Cukierman, Ana Lea 3-Chloro-2- hydroxypropyltrimethylammonium chloride Agarose Cationic polysaccharide Cationization 3-chloro-2-hydroxypropyltrimethylammonium chloride Agarose Alkoxides Cationic polysaccharide Cationization Cationized Degrees of substitution Electrospray ionization mass spectrometry Higher-degree Large-scale applications Maximum degree Methanolysis NMR spectroscopy Optimum concentration Reaction parameters Reaction time Scanning electronic microscopy Substitution degree Viscosimetry Chlorine compounds Electrospray ionization Infrared spectroscopy Mass spectrometry Molecular weight Nuclear magnetic resonance spectroscopy Scales (weighing instruments) agarose epoxide galactose polysaccharide article carbon nuclear magnetic resonance chemical structure electrospray mass spectrometry infrared spectroscopy methanolysis priority journal proton nuclear magnetic resonance scanning electron microscopy substitution reaction viscometry Borohydrides Cations Infrared Rays Magnetic Resonance Spectroscopy Microscopy, Electron, Scanning Propanols Quaternary Ammonium Compounds Sepharose Spectrometry, Mass, Electrospray Ionization Spectrophotometry, Infrared Viscosity Cationized agaroses with different degrees of substitution (0.04-0.77) were synthesized, employing 3-chloro-2-hydroxypropyltrimethylammonium chloride (CHPTAC). The influence of different reaction parameters on the substitution degree and molecular weight was evaluated. The investigated parameters were concentration of reagents, temperature, time, and addition of NaBH4. The products were characterized by means of scanning electronic microscopy, infrared spectroscopy, viscosimetry, and NMR spectroscopy. Methanolysis products were studied by electrospray ionization mass spectrometry. The higher the concentration of CHPTAC employed, a higher degree of substitution was obtained, if the optimum concentration of NaOH in each case was employed. Insufficient quantities of NaOH reduced epoxide formation and the reacting alkoxides of the polysaccharide, whereas an excess of NaOH favored degradation of the epoxide and decrease in the molecular weight of the product. A reaction time of 2 h was sufficient to obtain products with the maximum degree of substitution for each case. The addition of NaBH4 gave products with a slightly higher molecular weight, but the extra cost involved should not justify its use for large-scale application. © 2010 Elsevier Ltd. All rights reserved. Fil:Prado, H.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Matulewicz, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bonelli, P.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cukierman, A.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2011 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00086215_v346_n2_p311_Prado http://hdl.handle.net/20.500.12110/paper_00086215_v346_n2_p311_Prado
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	3-Chloro-2- hydroxypropyltrimethylammonium chloride Agarose Cationic polysaccharide Cationization 3-chloro-2-hydroxypropyltrimethylammonium chloride Agarose Alkoxides Cationic polysaccharide Cationization Cationized Degrees of substitution Electrospray ionization mass spectrometry Higher-degree Large-scale applications Maximum degree Methanolysis NMR spectroscopy Optimum concentration Reaction parameters Reaction time Scanning electronic microscopy Substitution degree Viscosimetry Chlorine compounds Electrospray ionization Infrared spectroscopy Mass spectrometry Molecular weight Nuclear magnetic resonance spectroscopy Scales (weighing instruments) agarose epoxide galactose polysaccharide article carbon nuclear magnetic resonance chemical structure electrospray mass spectrometry infrared spectroscopy methanolysis priority journal proton nuclear magnetic resonance scanning electron microscopy substitution reaction viscometry Borohydrides Cations Infrared Rays Magnetic Resonance Spectroscopy Microscopy, Electron, Scanning Propanols Quaternary Ammonium Compounds Sepharose Spectrometry, Mass, Electrospray Ionization Spectrophotometry, Infrared Viscosity
spellingShingle	3-Chloro-2- hydroxypropyltrimethylammonium chloride Agarose Cationic polysaccharide Cationization 3-chloro-2-hydroxypropyltrimethylammonium chloride Agarose Alkoxides Cationic polysaccharide Cationization Cationized Degrees of substitution Electrospray ionization mass spectrometry Higher-degree Large-scale applications Maximum degree Methanolysis NMR spectroscopy Optimum concentration Reaction parameters Reaction time Scanning electronic microscopy Substitution degree Viscosimetry Chlorine compounds Electrospray ionization Infrared spectroscopy Mass spectrometry Molecular weight Nuclear magnetic resonance spectroscopy Scales (weighing instruments) agarose epoxide galactose polysaccharide article carbon nuclear magnetic resonance chemical structure electrospray mass spectrometry infrared spectroscopy methanolysis priority journal proton nuclear magnetic resonance scanning electron microscopy substitution reaction viscometry Borohydrides Cations Infrared Rays Magnetic Resonance Spectroscopy Microscopy, Electron, Scanning Propanols Quaternary Ammonium Compounds Sepharose Spectrometry, Mass, Electrospray Ionization Spectrophotometry, Infrared Viscosity Prado, Héctor Juan Matulewicz, María Cristina Bonelli, Pablo R. Cukierman, Ana Lea Studies on the cationization of agarose
topic_facet	3-Chloro-2- hydroxypropyltrimethylammonium chloride Agarose Cationic polysaccharide Cationization 3-chloro-2-hydroxypropyltrimethylammonium chloride Agarose Alkoxides Cationic polysaccharide Cationization Cationized Degrees of substitution Electrospray ionization mass spectrometry Higher-degree Large-scale applications Maximum degree Methanolysis NMR spectroscopy Optimum concentration Reaction parameters Reaction time Scanning electronic microscopy Substitution degree Viscosimetry Chlorine compounds Electrospray ionization Infrared spectroscopy Mass spectrometry Molecular weight Nuclear magnetic resonance spectroscopy Scales (weighing instruments) agarose epoxide galactose polysaccharide article carbon nuclear magnetic resonance chemical structure electrospray mass spectrometry infrared spectroscopy methanolysis priority journal proton nuclear magnetic resonance scanning electron microscopy substitution reaction viscometry Borohydrides Cations Infrared Rays Magnetic Resonance Spectroscopy Microscopy, Electron, Scanning Propanols Quaternary Ammonium Compounds Sepharose Spectrometry, Mass, Electrospray Ionization Spectrophotometry, Infrared Viscosity
description	Cationized agaroses with different degrees of substitution (0.04-0.77) were synthesized, employing 3-chloro-2-hydroxypropyltrimethylammonium chloride (CHPTAC). The influence of different reaction parameters on the substitution degree and molecular weight was evaluated. The investigated parameters were concentration of reagents, temperature, time, and addition of NaBH4. The products were characterized by means of scanning electronic microscopy, infrared spectroscopy, viscosimetry, and NMR spectroscopy. Methanolysis products were studied by electrospray ionization mass spectrometry. The higher the concentration of CHPTAC employed, a higher degree of substitution was obtained, if the optimum concentration of NaOH in each case was employed. Insufficient quantities of NaOH reduced epoxide formation and the reacting alkoxides of the polysaccharide, whereas an excess of NaOH favored degradation of the epoxide and decrease in the molecular weight of the product. A reaction time of 2 h was sufficient to obtain products with the maximum degree of substitution for each case. The addition of NaBH4 gave products with a slightly higher molecular weight, but the extra cost involved should not justify its use for large-scale application. © 2010 Elsevier Ltd. All rights reserved.
author	Prado, Héctor Juan Matulewicz, María Cristina Bonelli, Pablo R. Cukierman, Ana Lea
author_facet	Prado, Héctor Juan Matulewicz, María Cristina Bonelli, Pablo R. Cukierman, Ana Lea
author_sort	Prado, Héctor Juan
title	Studies on the cationization of agarose
title_short	Studies on the cationization of agarose
title_full	Studies on the cationization of agarose
title_fullStr	Studies on the cationization of agarose
title_full_unstemmed	Studies on the cationization of agarose
title_sort	studies on the cationization of agarose
publishDate	2011
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00086215_v346_n2_p311_Prado http://hdl.handle.net/20.500.12110/paper_00086215_v346_n2_p311_Prado
work_keys_str_mv	AT pradohectorjuan studiesonthecationizationofagarose AT matulewiczmariacristina studiesonthecationizationofagarose AT bonellipablor studiesonthecationizationofagarose AT cukiermananalea studiesonthecationizationofagarose
_version_	1768545033078702080

Studies on the cationization of agarose

Ejemplares similares