Galactans from cystocarpic plants of the red seaweed Callophyllis variegata (Kallymeniaceae, Gigartinales)

The polysaccharide extracted from cystocarpic Callophyllis variegata was fractionated with potassium chloride yielding three small fractions that precipitated in the ranges of 0-0.05 M KCl, 1.20-1.25 M KCl, and 1.80-2.00 M KCl, and a main product soluble in 2.00 M KCl. These fractions were analyzed,...

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Autores principales: Rodriguez, María Cecilia, Pujol, Carlos Alberto, Damonte, Elsa Beatriz, Cerezo, Alberto Saúl, Matulewicz, María Cristina
Publicado: 2005
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Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00086215_v340_n18_p2742_Rodriguez
http://hdl.handle.net/20.500.12110/paper_00086215_v340_n18_p2742_Rodriguez
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spelling paper:paper_00086215_v340_n18_p2742_Rodriguez2023-06-08T14:32:49Z Galactans from cystocarpic plants of the red seaweed Callophyllis variegata (Kallymeniaceae, Gigartinales) Rodriguez, María Cecilia Pujol, Carlos Alberto Damonte, Elsa Beatriz Cerezo, Alberto Saúl Matulewicz, María Cristina Antiviral activity Callophyllis variegata Carrageenans Kallymeniaceae Red algal polysaccharides Sulfated galactans Antiviral activity Galactans Nuclear magnetic resonance spectroscopy Organic compounds Potassium compounds Carbohydrates antivirus agent carrageenan galactan galactose polysaccharide potassium chloride protein sulfate antiviral activity article carbon nuclear magnetic resonance chemical analysis Dengue virus drug isolation fractionation Herpes simplex virus 1 Herpes simplex virus 2 infrared spectroscopy methylation nuclear magnetic resonance spectroscopy plant precipitation priority journal seaweed structure analysis sulfation Antiviral Agents Carbon Isotopes Galactans Magnetic Resonance Spectroscopy Plant Extracts Seaweed Sodium Hydroxide Spectrophotometry, Infrared algae Callophyllis variegata Dengue virus Gigartinales Herpes Human herpesvirus 1 Human herpesvirus 2 Kallymeniaceae The polysaccharide extracted from cystocarpic Callophyllis variegata was fractionated with potassium chloride yielding three small fractions that precipitated in the ranges of 0-0.05 M KCl, 1.20-1.25 M KCl, and 1.80-2.00 M KCl, and a main product soluble in 2.00 M KCl. These fractions were analyzed, and as the first one contained very high amounts of protein, it was not studied further. Structural analysis of the rest of the fractions (F1-F3) was carried out by methylation, desulfation-methylation, IR, and 13C NMR spectroscopy. The results are consistent for F1 with a carrageenan-type backbone mainly constituted by β-D-galactose 2-sulfate linked to α-D-galactose 2,3,6-trisulfate and β-D-galactose 2,4-disulfate linked to 3,6-anhydro-D-galactose 2-sulfate as dominant diads. In F2 these diads are present together with low amounts of β-D-galactose 2-sulfate linked to 3,6-anhydro-D-galactose 2-sulfate, whose contribution becomes higher in F3. In addition, minor but significant amounts of L-galactose were detected. F1-F3 showed potent antiviral activity against herpes simplex types 1 and 2 and dengue type 2. © 2005 Elsevier Ltd. All rights reserved. Fil:Rodríguez, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pujol, C.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Damonte, E.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cerezo, A.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Matulewicz, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2005 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00086215_v340_n18_p2742_Rodriguez http://hdl.handle.net/20.500.12110/paper_00086215_v340_n18_p2742_Rodriguez
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Antiviral activity
Callophyllis variegata
Carrageenans
Kallymeniaceae
Red algal polysaccharides
Sulfated galactans
Antiviral activity
Galactans
Nuclear magnetic resonance spectroscopy
Organic compounds
Potassium compounds
Carbohydrates
antivirus agent
carrageenan
galactan
galactose
polysaccharide
potassium chloride
protein
sulfate
antiviral activity
article
carbon nuclear magnetic resonance
chemical analysis
Dengue virus
drug isolation
fractionation
Herpes simplex virus 1
Herpes simplex virus 2
infrared spectroscopy
methylation
nuclear magnetic resonance spectroscopy
plant
precipitation
priority journal
seaweed
structure analysis
sulfation
Antiviral Agents
Carbon Isotopes
Galactans
Magnetic Resonance Spectroscopy
Plant Extracts
Seaweed
Sodium Hydroxide
Spectrophotometry, Infrared
algae
Callophyllis variegata
Dengue virus
Gigartinales
Herpes
Human herpesvirus 1
Human herpesvirus 2
Kallymeniaceae
spellingShingle Antiviral activity
Callophyllis variegata
Carrageenans
Kallymeniaceae
Red algal polysaccharides
Sulfated galactans
Antiviral activity
Galactans
Nuclear magnetic resonance spectroscopy
Organic compounds
Potassium compounds
Carbohydrates
antivirus agent
carrageenan
galactan
galactose
polysaccharide
potassium chloride
protein
sulfate
antiviral activity
article
carbon nuclear magnetic resonance
chemical analysis
Dengue virus
drug isolation
fractionation
Herpes simplex virus 1
Herpes simplex virus 2
infrared spectroscopy
methylation
nuclear magnetic resonance spectroscopy
plant
precipitation
priority journal
seaweed
structure analysis
sulfation
Antiviral Agents
Carbon Isotopes
Galactans
Magnetic Resonance Spectroscopy
Plant Extracts
Seaweed
Sodium Hydroxide
Spectrophotometry, Infrared
algae
Callophyllis variegata
Dengue virus
Gigartinales
Herpes
Human herpesvirus 1
Human herpesvirus 2
Kallymeniaceae
Rodriguez, María Cecilia
Pujol, Carlos Alberto
Damonte, Elsa Beatriz
Cerezo, Alberto Saúl
Matulewicz, María Cristina
Galactans from cystocarpic plants of the red seaweed Callophyllis variegata (Kallymeniaceae, Gigartinales)
topic_facet Antiviral activity
Callophyllis variegata
Carrageenans
Kallymeniaceae
Red algal polysaccharides
Sulfated galactans
Antiviral activity
Galactans
Nuclear magnetic resonance spectroscopy
Organic compounds
Potassium compounds
Carbohydrates
antivirus agent
carrageenan
galactan
galactose
polysaccharide
potassium chloride
protein
sulfate
antiviral activity
article
carbon nuclear magnetic resonance
chemical analysis
Dengue virus
drug isolation
fractionation
Herpes simplex virus 1
Herpes simplex virus 2
infrared spectroscopy
methylation
nuclear magnetic resonance spectroscopy
plant
precipitation
priority journal
seaweed
structure analysis
sulfation
Antiviral Agents
Carbon Isotopes
Galactans
Magnetic Resonance Spectroscopy
Plant Extracts
Seaweed
Sodium Hydroxide
Spectrophotometry, Infrared
algae
Callophyllis variegata
Dengue virus
Gigartinales
Herpes
Human herpesvirus 1
Human herpesvirus 2
Kallymeniaceae
description The polysaccharide extracted from cystocarpic Callophyllis variegata was fractionated with potassium chloride yielding three small fractions that precipitated in the ranges of 0-0.05 M KCl, 1.20-1.25 M KCl, and 1.80-2.00 M KCl, and a main product soluble in 2.00 M KCl. These fractions were analyzed, and as the first one contained very high amounts of protein, it was not studied further. Structural analysis of the rest of the fractions (F1-F3) was carried out by methylation, desulfation-methylation, IR, and 13C NMR spectroscopy. The results are consistent for F1 with a carrageenan-type backbone mainly constituted by β-D-galactose 2-sulfate linked to α-D-galactose 2,3,6-trisulfate and β-D-galactose 2,4-disulfate linked to 3,6-anhydro-D-galactose 2-sulfate as dominant diads. In F2 these diads are present together with low amounts of β-D-galactose 2-sulfate linked to 3,6-anhydro-D-galactose 2-sulfate, whose contribution becomes higher in F3. In addition, minor but significant amounts of L-galactose were detected. F1-F3 showed potent antiviral activity against herpes simplex types 1 and 2 and dengue type 2. © 2005 Elsevier Ltd. All rights reserved.
author Rodriguez, María Cecilia
Pujol, Carlos Alberto
Damonte, Elsa Beatriz
Cerezo, Alberto Saúl
Matulewicz, María Cristina
author_facet Rodriguez, María Cecilia
Pujol, Carlos Alberto
Damonte, Elsa Beatriz
Cerezo, Alberto Saúl
Matulewicz, María Cristina
author_sort Rodriguez, María Cecilia
title Galactans from cystocarpic plants of the red seaweed Callophyllis variegata (Kallymeniaceae, Gigartinales)
title_short Galactans from cystocarpic plants of the red seaweed Callophyllis variegata (Kallymeniaceae, Gigartinales)
title_full Galactans from cystocarpic plants of the red seaweed Callophyllis variegata (Kallymeniaceae, Gigartinales)
title_fullStr Galactans from cystocarpic plants of the red seaweed Callophyllis variegata (Kallymeniaceae, Gigartinales)
title_full_unstemmed Galactans from cystocarpic plants of the red seaweed Callophyllis variegata (Kallymeniaceae, Gigartinales)
title_sort galactans from cystocarpic plants of the red seaweed callophyllis variegata (kallymeniaceae, gigartinales)
publishDate 2005
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00086215_v340_n18_p2742_Rodriguez
http://hdl.handle.net/20.500.12110/paper_00086215_v340_n18_p2742_Rodriguez
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