Galactans from cystocarpic plants of the red seaweed Callophyllis variegata (Kallymeniaceae, Gigartinales)
The polysaccharide extracted from cystocarpic Callophyllis variegata was fractionated with potassium chloride yielding three small fractions that precipitated in the ranges of 0-0.05 M KCl, 1.20-1.25 M KCl, and 1.80-2.00 M KCl, and a main product soluble in 2.00 M KCl. These fractions were analyzed,...
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2005
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Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00086215_v340_n18_p2742_Rodriguez http://hdl.handle.net/20.500.12110/paper_00086215_v340_n18_p2742_Rodriguez |
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paper:paper_00086215_v340_n18_p2742_Rodriguez2023-06-08T14:32:49Z Galactans from cystocarpic plants of the red seaweed Callophyllis variegata (Kallymeniaceae, Gigartinales) Rodriguez, María Cecilia Pujol, Carlos Alberto Damonte, Elsa Beatriz Cerezo, Alberto Saúl Matulewicz, María Cristina Antiviral activity Callophyllis variegata Carrageenans Kallymeniaceae Red algal polysaccharides Sulfated galactans Antiviral activity Galactans Nuclear magnetic resonance spectroscopy Organic compounds Potassium compounds Carbohydrates antivirus agent carrageenan galactan galactose polysaccharide potassium chloride protein sulfate antiviral activity article carbon nuclear magnetic resonance chemical analysis Dengue virus drug isolation fractionation Herpes simplex virus 1 Herpes simplex virus 2 infrared spectroscopy methylation nuclear magnetic resonance spectroscopy plant precipitation priority journal seaweed structure analysis sulfation Antiviral Agents Carbon Isotopes Galactans Magnetic Resonance Spectroscopy Plant Extracts Seaweed Sodium Hydroxide Spectrophotometry, Infrared algae Callophyllis variegata Dengue virus Gigartinales Herpes Human herpesvirus 1 Human herpesvirus 2 Kallymeniaceae The polysaccharide extracted from cystocarpic Callophyllis variegata was fractionated with potassium chloride yielding three small fractions that precipitated in the ranges of 0-0.05 M KCl, 1.20-1.25 M KCl, and 1.80-2.00 M KCl, and a main product soluble in 2.00 M KCl. These fractions were analyzed, and as the first one contained very high amounts of protein, it was not studied further. Structural analysis of the rest of the fractions (F1-F3) was carried out by methylation, desulfation-methylation, IR, and 13C NMR spectroscopy. The results are consistent for F1 with a carrageenan-type backbone mainly constituted by β-D-galactose 2-sulfate linked to α-D-galactose 2,3,6-trisulfate and β-D-galactose 2,4-disulfate linked to 3,6-anhydro-D-galactose 2-sulfate as dominant diads. In F2 these diads are present together with low amounts of β-D-galactose 2-sulfate linked to 3,6-anhydro-D-galactose 2-sulfate, whose contribution becomes higher in F3. In addition, minor but significant amounts of L-galactose were detected. F1-F3 showed potent antiviral activity against herpes simplex types 1 and 2 and dengue type 2. © 2005 Elsevier Ltd. All rights reserved. Fil:Rodríguez, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pujol, C.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Damonte, E.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cerezo, A.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Matulewicz, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2005 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00086215_v340_n18_p2742_Rodriguez http://hdl.handle.net/20.500.12110/paper_00086215_v340_n18_p2742_Rodriguez |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
Antiviral activity Callophyllis variegata Carrageenans Kallymeniaceae Red algal polysaccharides Sulfated galactans Antiviral activity Galactans Nuclear magnetic resonance spectroscopy Organic compounds Potassium compounds Carbohydrates antivirus agent carrageenan galactan galactose polysaccharide potassium chloride protein sulfate antiviral activity article carbon nuclear magnetic resonance chemical analysis Dengue virus drug isolation fractionation Herpes simplex virus 1 Herpes simplex virus 2 infrared spectroscopy methylation nuclear magnetic resonance spectroscopy plant precipitation priority journal seaweed structure analysis sulfation Antiviral Agents Carbon Isotopes Galactans Magnetic Resonance Spectroscopy Plant Extracts Seaweed Sodium Hydroxide Spectrophotometry, Infrared algae Callophyllis variegata Dengue virus Gigartinales Herpes Human herpesvirus 1 Human herpesvirus 2 Kallymeniaceae |
spellingShingle |
Antiviral activity Callophyllis variegata Carrageenans Kallymeniaceae Red algal polysaccharides Sulfated galactans Antiviral activity Galactans Nuclear magnetic resonance spectroscopy Organic compounds Potassium compounds Carbohydrates antivirus agent carrageenan galactan galactose polysaccharide potassium chloride protein sulfate antiviral activity article carbon nuclear magnetic resonance chemical analysis Dengue virus drug isolation fractionation Herpes simplex virus 1 Herpes simplex virus 2 infrared spectroscopy methylation nuclear magnetic resonance spectroscopy plant precipitation priority journal seaweed structure analysis sulfation Antiviral Agents Carbon Isotopes Galactans Magnetic Resonance Spectroscopy Plant Extracts Seaweed Sodium Hydroxide Spectrophotometry, Infrared algae Callophyllis variegata Dengue virus Gigartinales Herpes Human herpesvirus 1 Human herpesvirus 2 Kallymeniaceae Rodriguez, María Cecilia Pujol, Carlos Alberto Damonte, Elsa Beatriz Cerezo, Alberto Saúl Matulewicz, María Cristina Galactans from cystocarpic plants of the red seaweed Callophyllis variegata (Kallymeniaceae, Gigartinales) |
topic_facet |
Antiviral activity Callophyllis variegata Carrageenans Kallymeniaceae Red algal polysaccharides Sulfated galactans Antiviral activity Galactans Nuclear magnetic resonance spectroscopy Organic compounds Potassium compounds Carbohydrates antivirus agent carrageenan galactan galactose polysaccharide potassium chloride protein sulfate antiviral activity article carbon nuclear magnetic resonance chemical analysis Dengue virus drug isolation fractionation Herpes simplex virus 1 Herpes simplex virus 2 infrared spectroscopy methylation nuclear magnetic resonance spectroscopy plant precipitation priority journal seaweed structure analysis sulfation Antiviral Agents Carbon Isotopes Galactans Magnetic Resonance Spectroscopy Plant Extracts Seaweed Sodium Hydroxide Spectrophotometry, Infrared algae Callophyllis variegata Dengue virus Gigartinales Herpes Human herpesvirus 1 Human herpesvirus 2 Kallymeniaceae |
description |
The polysaccharide extracted from cystocarpic Callophyllis variegata was fractionated with potassium chloride yielding three small fractions that precipitated in the ranges of 0-0.05 M KCl, 1.20-1.25 M KCl, and 1.80-2.00 M KCl, and a main product soluble in 2.00 M KCl. These fractions were analyzed, and as the first one contained very high amounts of protein, it was not studied further. Structural analysis of the rest of the fractions (F1-F3) was carried out by methylation, desulfation-methylation, IR, and 13C NMR spectroscopy. The results are consistent for F1 with a carrageenan-type backbone mainly constituted by β-D-galactose 2-sulfate linked to α-D-galactose 2,3,6-trisulfate and β-D-galactose 2,4-disulfate linked to 3,6-anhydro-D-galactose 2-sulfate as dominant diads. In F2 these diads are present together with low amounts of β-D-galactose 2-sulfate linked to 3,6-anhydro-D-galactose 2-sulfate, whose contribution becomes higher in F3. In addition, minor but significant amounts of L-galactose were detected. F1-F3 showed potent antiviral activity against herpes simplex types 1 and 2 and dengue type 2. © 2005 Elsevier Ltd. All rights reserved. |
author |
Rodriguez, María Cecilia Pujol, Carlos Alberto Damonte, Elsa Beatriz Cerezo, Alberto Saúl Matulewicz, María Cristina |
author_facet |
Rodriguez, María Cecilia Pujol, Carlos Alberto Damonte, Elsa Beatriz Cerezo, Alberto Saúl Matulewicz, María Cristina |
author_sort |
Rodriguez, María Cecilia |
title |
Galactans from cystocarpic plants of the red seaweed Callophyllis variegata (Kallymeniaceae, Gigartinales) |
title_short |
Galactans from cystocarpic plants of the red seaweed Callophyllis variegata (Kallymeniaceae, Gigartinales) |
title_full |
Galactans from cystocarpic plants of the red seaweed Callophyllis variegata (Kallymeniaceae, Gigartinales) |
title_fullStr |
Galactans from cystocarpic plants of the red seaweed Callophyllis variegata (Kallymeniaceae, Gigartinales) |
title_full_unstemmed |
Galactans from cystocarpic plants of the red seaweed Callophyllis variegata (Kallymeniaceae, Gigartinales) |
title_sort |
galactans from cystocarpic plants of the red seaweed callophyllis variegata (kallymeniaceae, gigartinales) |
publishDate |
2005 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00086215_v340_n18_p2742_Rodriguez http://hdl.handle.net/20.500.12110/paper_00086215_v340_n18_p2742_Rodriguez |
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1768542767415296000 |