Unusual structures in the polysaccharides from the red seaweed Pterocladiella capillacea (Gelidiaceae, Gelidiales)
Sequential extraction of tetrasporic Pterocladiella capillacea with water at room temperature and then at 50°C led to the isolation of two products that were each fractionated with cetrimide to give a soluble fraction and a precipitate. The precipitates were then subjected to fractional solubilizati...
| Autores principales: | , |
|---|---|
| Publicado: |
2003
|
| Materias: | |
| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00086215_v338_n9_p943_Errea http://hdl.handle.net/20.500.12110/paper_00086215_v338_n9_p943_Errea |
| Aporte de: |
| id |
paper:paper_00086215_v338_n9_p943_Errea |
|---|---|
| record_format |
dspace |
| spelling |
paper:paper_00086215_v338_n9_p943_Errea2023-06-08T14:32:45Z Unusual structures in the polysaccharides from the red seaweed Pterocladiella capillacea (Gelidiaceae, Gelidiales) Errea, María Inés Matulewicz, María Cristina Agarans Galactans, D-galactose blocks Galactans, sulfated Gelidiales Polysaccharide, red algal Pterocladiella capillacea Fractionation Liquid chromatography Molecular structure Nuclear magnetic resonance spectroscopy Plants (botany) Substitution reactions Weed control Sequential extraction Polysaccharides 2,6 di o methylgalactose 3,6 anhydrogalactose cetrimide polysaccharide sodium chloride unclassified drug alga anion exchange chromatography article carbohydrate analysis carbohydrate synthesis carbon nuclear magnetic resonance desulfurization fractionation isolation procedure methylation molecular weight nonhuman precipitation priority journal pterocladiella capillacea room temperature seaweed solubilization structure analysis algae Gelidiaceae Gelidiales Pterocladiella Pterocladiella capillacea Pterocladiella capillacea Sequential extraction of tetrasporic Pterocladiella capillacea with water at room temperature and then at 50°C led to the isolation of two products that were each fractionated with cetrimide to give a soluble fraction and a precipitate. The precipitates were then subjected to fractional solubilization in solutions of increasing sodium chloride concentration. The whole treatment yielded two major fractions in each case, one soluble in the cetrimide medium and the other soluble in 0.5 M NaCl, which were further fractionated by anion-exchange chromatography. Structural analysis, carried out by methylation, desulfation-methylation, 13C NMR spectroscopy and determination of the absolute configuration of the 2,6-di-O-methylgalactose units in the permethylated products, indicated the presence of xylogalactans, with low content of 3,6-anhydrogalactose and low molecular weight. These polysaccharides varied in the level of xylopyranosyl and sulfate substitution, primarily on the 6-position of the 3-linked β-D-galactopyranosyl and on the 3-position of the 4-linked α-galactopyranosyl units. Moreover, herein we report, for the first time, the presence of 3-substituted, 4-linked D-galactopyranosyl residues in an alga belonging to the Gelidiales. © 2003 Elsevier Science Ltd. All rights reserved. Fil:Errea, M.I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Matulewicz, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2003 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00086215_v338_n9_p943_Errea http://hdl.handle.net/20.500.12110/paper_00086215_v338_n9_p943_Errea |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Agarans Galactans, D-galactose blocks Galactans, sulfated Gelidiales Polysaccharide, red algal Pterocladiella capillacea Fractionation Liquid chromatography Molecular structure Nuclear magnetic resonance spectroscopy Plants (botany) Substitution reactions Weed control Sequential extraction Polysaccharides 2,6 di o methylgalactose 3,6 anhydrogalactose cetrimide polysaccharide sodium chloride unclassified drug alga anion exchange chromatography article carbohydrate analysis carbohydrate synthesis carbon nuclear magnetic resonance desulfurization fractionation isolation procedure methylation molecular weight nonhuman precipitation priority journal pterocladiella capillacea room temperature seaweed solubilization structure analysis algae Gelidiaceae Gelidiales Pterocladiella Pterocladiella capillacea Pterocladiella capillacea |
| spellingShingle |
Agarans Galactans, D-galactose blocks Galactans, sulfated Gelidiales Polysaccharide, red algal Pterocladiella capillacea Fractionation Liquid chromatography Molecular structure Nuclear magnetic resonance spectroscopy Plants (botany) Substitution reactions Weed control Sequential extraction Polysaccharides 2,6 di o methylgalactose 3,6 anhydrogalactose cetrimide polysaccharide sodium chloride unclassified drug alga anion exchange chromatography article carbohydrate analysis carbohydrate synthesis carbon nuclear magnetic resonance desulfurization fractionation isolation procedure methylation molecular weight nonhuman precipitation priority journal pterocladiella capillacea room temperature seaweed solubilization structure analysis algae Gelidiaceae Gelidiales Pterocladiella Pterocladiella capillacea Pterocladiella capillacea Errea, María Inés Matulewicz, María Cristina Unusual structures in the polysaccharides from the red seaweed Pterocladiella capillacea (Gelidiaceae, Gelidiales) |
| topic_facet |
Agarans Galactans, D-galactose blocks Galactans, sulfated Gelidiales Polysaccharide, red algal Pterocladiella capillacea Fractionation Liquid chromatography Molecular structure Nuclear magnetic resonance spectroscopy Plants (botany) Substitution reactions Weed control Sequential extraction Polysaccharides 2,6 di o methylgalactose 3,6 anhydrogalactose cetrimide polysaccharide sodium chloride unclassified drug alga anion exchange chromatography article carbohydrate analysis carbohydrate synthesis carbon nuclear magnetic resonance desulfurization fractionation isolation procedure methylation molecular weight nonhuman precipitation priority journal pterocladiella capillacea room temperature seaweed solubilization structure analysis algae Gelidiaceae Gelidiales Pterocladiella Pterocladiella capillacea Pterocladiella capillacea |
| description |
Sequential extraction of tetrasporic Pterocladiella capillacea with water at room temperature and then at 50°C led to the isolation of two products that were each fractionated with cetrimide to give a soluble fraction and a precipitate. The precipitates were then subjected to fractional solubilization in solutions of increasing sodium chloride concentration. The whole treatment yielded two major fractions in each case, one soluble in the cetrimide medium and the other soluble in 0.5 M NaCl, which were further fractionated by anion-exchange chromatography. Structural analysis, carried out by methylation, desulfation-methylation, 13C NMR spectroscopy and determination of the absolute configuration of the 2,6-di-O-methylgalactose units in the permethylated products, indicated the presence of xylogalactans, with low content of 3,6-anhydrogalactose and low molecular weight. These polysaccharides varied in the level of xylopyranosyl and sulfate substitution, primarily on the 6-position of the 3-linked β-D-galactopyranosyl and on the 3-position of the 4-linked α-galactopyranosyl units. Moreover, herein we report, for the first time, the presence of 3-substituted, 4-linked D-galactopyranosyl residues in an alga belonging to the Gelidiales. © 2003 Elsevier Science Ltd. All rights reserved. |
| author |
Errea, María Inés Matulewicz, María Cristina |
| author_facet |
Errea, María Inés Matulewicz, María Cristina |
| author_sort |
Errea, María Inés |
| title |
Unusual structures in the polysaccharides from the red seaweed Pterocladiella capillacea (Gelidiaceae, Gelidiales) |
| title_short |
Unusual structures in the polysaccharides from the red seaweed Pterocladiella capillacea (Gelidiaceae, Gelidiales) |
| title_full |
Unusual structures in the polysaccharides from the red seaweed Pterocladiella capillacea (Gelidiaceae, Gelidiales) |
| title_fullStr |
Unusual structures in the polysaccharides from the red seaweed Pterocladiella capillacea (Gelidiaceae, Gelidiales) |
| title_full_unstemmed |
Unusual structures in the polysaccharides from the red seaweed Pterocladiella capillacea (Gelidiaceae, Gelidiales) |
| title_sort |
unusual structures in the polysaccharides from the red seaweed pterocladiella capillacea (gelidiaceae, gelidiales) |
| publishDate |
2003 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00086215_v338_n9_p943_Errea http://hdl.handle.net/20.500.12110/paper_00086215_v338_n9_p943_Errea |
| work_keys_str_mv |
AT erreamariaines unusualstructuresinthepolysaccharidesfromtheredseaweedpterocladiellacapillaceagelidiaceaegelidiales AT matulewiczmariacristina unusualstructuresinthepolysaccharidesfromtheredseaweedpterocladiellacapillaceagelidiaceaegelidiales |
| _version_ |
1768542345319415808 |