Influence of exo β-D-galactofuranosidase inhibitors in cultures of Penicillium fellutanum and modifications in hyphal cell structure
We have examined β-D-galactofuranosidase production by Penicillium fellutanum in the presence of D-galactono-1,4-lactone or 4-aminophenyl 1-thio-β-D-galactofuranoside, two potent in vitro inhibitors of the enzyme. Activity of the enzyme in the culture filtrate was increased by 35% when glucose was r...
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2002
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00086215_v337_n10_p891_Marino http://hdl.handle.net/20.500.12110/paper_00086215_v337_n10_p891_Marino |
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paper:paper_00086215_v337_n10_p891_Marino2023-06-08T14:32:40Z Influence of exo β-D-galactofuranosidase inhibitors in cultures of Penicillium fellutanum and modifications in hyphal cell structure Galactofuranose Galactofuranosidase Inhibitors Penicillium fellutanum Cell culture Enzymes Filtration Glucose Proteins Transmission electron microscopy Penicillium fellutanum Carbohydrates beta dextro galactofuranosidase inhibitor carbon enzyme galactomannan galactose glucose ribose sugar unclassified drug article cell structure cell wall controlled study enzyme activity enzyme synthesis fungus culture fungus growth fungus hyphae microscopy mycelium nonhuman Penicillium priority journal protein aggregation transmission electron microscopy beta-Galactosidase Cell Wall Enzyme Inhibitors Galactose Glycoside Hydrolases Hyphae Microscopy, Electron Penicillium Penicillium Penicillium fellutanum We have examined β-D-galactofuranosidase production by Penicillium fellutanum in the presence of D-galactono-1,4-lactone or 4-aminophenyl 1-thio-β-D-galactofuranoside, two potent in vitro inhibitors of the enzyme. Activity of the enzyme in the culture filtrate was increased by 35% when glucose was replaced by D-galactose as the carbon source, and the activity diminished 80% of the control value when the inhibitors were added. Significant alterations of the culture were observed: (a) the medium became increasingly opalescent due to the secretion of a protein aggregate (PA) which contained 15% neutral sugar, mainly ribose; (b) the peptidophosphogalactomannan (pPGM) containing galactofuranose, normally produced by P. fellutanum, could not be obtained from the culture medium in the presence of the inhibitors; (c) the content of galactofuranose in the cell wall was significantly decreased in the presence of D-galactono-1,4-lactone. The influence on the mycelia growth was investigated by light microscopy (LM) and transmission electron microscopy (TEM) showing important alterations. © 2002 Elsevier Science Ltd. All rights reserved. 2002 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00086215_v337_n10_p891_Marino http://hdl.handle.net/20.500.12110/paper_00086215_v337_n10_p891_Marino |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Galactofuranose Galactofuranosidase Inhibitors Penicillium fellutanum Cell culture Enzymes Filtration Glucose Proteins Transmission electron microscopy Penicillium fellutanum Carbohydrates beta dextro galactofuranosidase inhibitor carbon enzyme galactomannan galactose glucose ribose sugar unclassified drug article cell structure cell wall controlled study enzyme activity enzyme synthesis fungus culture fungus growth fungus hyphae microscopy mycelium nonhuman Penicillium priority journal protein aggregation transmission electron microscopy beta-Galactosidase Cell Wall Enzyme Inhibitors Galactose Glycoside Hydrolases Hyphae Microscopy, Electron Penicillium Penicillium Penicillium fellutanum |
| spellingShingle |
Galactofuranose Galactofuranosidase Inhibitors Penicillium fellutanum Cell culture Enzymes Filtration Glucose Proteins Transmission electron microscopy Penicillium fellutanum Carbohydrates beta dextro galactofuranosidase inhibitor carbon enzyme galactomannan galactose glucose ribose sugar unclassified drug article cell structure cell wall controlled study enzyme activity enzyme synthesis fungus culture fungus growth fungus hyphae microscopy mycelium nonhuman Penicillium priority journal protein aggregation transmission electron microscopy beta-Galactosidase Cell Wall Enzyme Inhibitors Galactose Glycoside Hydrolases Hyphae Microscopy, Electron Penicillium Penicillium Penicillium fellutanum Influence of exo β-D-galactofuranosidase inhibitors in cultures of Penicillium fellutanum and modifications in hyphal cell structure |
| topic_facet |
Galactofuranose Galactofuranosidase Inhibitors Penicillium fellutanum Cell culture Enzymes Filtration Glucose Proteins Transmission electron microscopy Penicillium fellutanum Carbohydrates beta dextro galactofuranosidase inhibitor carbon enzyme galactomannan galactose glucose ribose sugar unclassified drug article cell structure cell wall controlled study enzyme activity enzyme synthesis fungus culture fungus growth fungus hyphae microscopy mycelium nonhuman Penicillium priority journal protein aggregation transmission electron microscopy beta-Galactosidase Cell Wall Enzyme Inhibitors Galactose Glycoside Hydrolases Hyphae Microscopy, Electron Penicillium Penicillium Penicillium fellutanum |
| description |
We have examined β-D-galactofuranosidase production by Penicillium fellutanum in the presence of D-galactono-1,4-lactone or 4-aminophenyl 1-thio-β-D-galactofuranoside, two potent in vitro inhibitors of the enzyme. Activity of the enzyme in the culture filtrate was increased by 35% when glucose was replaced by D-galactose as the carbon source, and the activity diminished 80% of the control value when the inhibitors were added. Significant alterations of the culture were observed: (a) the medium became increasingly opalescent due to the secretion of a protein aggregate (PA) which contained 15% neutral sugar, mainly ribose; (b) the peptidophosphogalactomannan (pPGM) containing galactofuranose, normally produced by P. fellutanum, could not be obtained from the culture medium in the presence of the inhibitors; (c) the content of galactofuranose in the cell wall was significantly decreased in the presence of D-galactono-1,4-lactone. The influence on the mycelia growth was investigated by light microscopy (LM) and transmission electron microscopy (TEM) showing important alterations. © 2002 Elsevier Science Ltd. All rights reserved. |
| title |
Influence of exo β-D-galactofuranosidase inhibitors in cultures of Penicillium fellutanum and modifications in hyphal cell structure |
| title_short |
Influence of exo β-D-galactofuranosidase inhibitors in cultures of Penicillium fellutanum and modifications in hyphal cell structure |
| title_full |
Influence of exo β-D-galactofuranosidase inhibitors in cultures of Penicillium fellutanum and modifications in hyphal cell structure |
| title_fullStr |
Influence of exo β-D-galactofuranosidase inhibitors in cultures of Penicillium fellutanum and modifications in hyphal cell structure |
| title_full_unstemmed |
Influence of exo β-D-galactofuranosidase inhibitors in cultures of Penicillium fellutanum and modifications in hyphal cell structure |
| title_sort |
influence of exo β-d-galactofuranosidase inhibitors in cultures of penicillium fellutanum and modifications in hyphal cell structure |
| publishDate |
2002 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00086215_v337_n10_p891_Marino http://hdl.handle.net/20.500.12110/paper_00086215_v337_n10_p891_Marino |
| _version_ |
1768546378330406912 |