Structural studies on a glycopeptide from the tree fungus Cyttaria harioti Fischer
A glycopeptide (In1) was isolated by phenol-water extraction from Cyttaria harioti Fischer, parasite of Nothofagus sps. Neutral sugars account for 89% of In1 and were characterized as glucose, mannose, and galactose. Glucosamine, identified by g.l.c., was colorimetrically estimated (5.8%). The molar...
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1985
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Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00086215_v138_n2_p257_Oliva http://hdl.handle.net/20.500.12110/paper_00086215_v138_n2_p257_Oliva |
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paper:paper_00086215_v138_n2_p257_Oliva2023-06-08T14:32:14Z Structural studies on a glycopeptide from the tree fungus Cyttaria harioti Fischer Oliva, Elisa María Muchnik de Lederkremer, Rosa María A glycopeptide (In1) was isolated by phenol-water extraction from Cyttaria harioti Fischer, parasite of Nothofagus sps. Neutral sugars account for 89% of In1 and were characterized as glucose, mannose, and galactose. Glucosamine, identified by g.l.c., was colorimetrically estimated (5.8%). The molar ratio of Glc:Man:Gal:GlcNAc was 17:11:3:2. The linkages between the various monosaccharide residues were established through methylation analysis and periodate oxidation studies. The anomeric configurations of the various glycosyl groups were determined by chromium trioxide oxidation of the acetylated polysaccharide. The results were confirmed by 13C-n.m.r. spectroscopy. The sugar chain is N-glycosyl-linked to the peptide. Structural features of the carbohydrate moiety of glycopeptide In1 are described. © 1985. Fil:Oliva, E.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:de Lederkremer, R.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1985 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00086215_v138_n2_p257_Oliva http://hdl.handle.net/20.500.12110/paper_00086215_v138_n2_p257_Oliva |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
description |
A glycopeptide (In1) was isolated by phenol-water extraction from Cyttaria harioti Fischer, parasite of Nothofagus sps. Neutral sugars account for 89% of In1 and were characterized as glucose, mannose, and galactose. Glucosamine, identified by g.l.c., was colorimetrically estimated (5.8%). The molar ratio of Glc:Man:Gal:GlcNAc was 17:11:3:2. The linkages between the various monosaccharide residues were established through methylation analysis and periodate oxidation studies. The anomeric configurations of the various glycosyl groups were determined by chromium trioxide oxidation of the acetylated polysaccharide. The results were confirmed by 13C-n.m.r. spectroscopy. The sugar chain is N-glycosyl-linked to the peptide. Structural features of the carbohydrate moiety of glycopeptide In1 are described. © 1985. |
author |
Oliva, Elisa María Muchnik de Lederkremer, Rosa María |
spellingShingle |
Oliva, Elisa María Muchnik de Lederkremer, Rosa María Structural studies on a glycopeptide from the tree fungus Cyttaria harioti Fischer |
author_facet |
Oliva, Elisa María Muchnik de Lederkremer, Rosa María |
author_sort |
Oliva, Elisa María |
title |
Structural studies on a glycopeptide from the tree fungus Cyttaria harioti Fischer |
title_short |
Structural studies on a glycopeptide from the tree fungus Cyttaria harioti Fischer |
title_full |
Structural studies on a glycopeptide from the tree fungus Cyttaria harioti Fischer |
title_fullStr |
Structural studies on a glycopeptide from the tree fungus Cyttaria harioti Fischer |
title_full_unstemmed |
Structural studies on a glycopeptide from the tree fungus Cyttaria harioti Fischer |
title_sort |
structural studies on a glycopeptide from the tree fungus cyttaria harioti fischer |
publishDate |
1985 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00086215_v138_n2_p257_Oliva http://hdl.handle.net/20.500.12110/paper_00086215_v138_n2_p257_Oliva |
work_keys_str_mv |
AT olivaelisamaria structuralstudiesonaglycopeptidefromthetreefunguscyttariahariotifischer AT muchnikdelederkremerrosamaria structuralstudiesonaglycopeptidefromthetreefunguscyttariahariotifischer |
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1768544620523814912 |