Adrenal 11-beta hydroxysteroid dehydrogenase activity in response to stress

This work studied the effect of stresses produced by simulated gavage or gavage with 200 mmol/L HCl two hours before adrenal extraction, on the activities of the 11β-hydroxysteroid dehydrogenase 1 and 11β-hydroxysteroid dehydrogenase 2 isoforms present in the rat adrenal gland. These activities were...

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Autor principal:	Zallocchi, Marisa Laura
Publicado:	2004
Materias:	Acidosis Adrenal HSD Stress 11beta hydroxysteroid dehydrogenase adrenalin corticosterone dehydrocorticosterone glucocorticoid hydrochloric acid isoenzyme nicotinamide adenine dinucleotide nicotinamide adenine dinucleotide phosphate phenylethanolamine n methyltransferase adrenal gland animal experiment animal tissue article controlled study enzyme activation enzyme activity experimental rat feeding homeostasis male measurement microsome nonhuman priority journal protein synthesis rat simulation stress thin layer chromatography
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00084212_v82_n6_p422_Zallocchi http://hdl.handle.net/20.500.12110/paper_00084212_v82_n6_p422_Zallocchi
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00084212_v82_n6_p422_Zallocchi
record_format	dspace
spelling	paper:paper_00084212_v82_n6_p422_Zallocchi2023-06-08T14:32:02Z Adrenal 11-beta hydroxysteroid dehydrogenase activity in response to stress Zallocchi, Marisa Laura Acidosis Adrenal HSD Stress 11beta hydroxysteroid dehydrogenase adrenalin corticosterone dehydrocorticosterone glucocorticoid hydrochloric acid isoenzyme nicotinamide adenine dinucleotide nicotinamide adenine dinucleotide phosphate phenylethanolamine n methyltransferase adrenal gland animal experiment animal tissue article controlled study enzyme activation enzyme activity experimental rat feeding homeostasis male measurement microsome nonhuman priority journal protein synthesis rat simulation stress thin layer chromatography This work studied the effect of stresses produced by simulated gavage or gavage with 200 mmol/L HCl two hours before adrenal extraction, on the activities of the 11β-hydroxysteroid dehydrogenase 1 and 11β-hydroxysteroid dehydrogenase 2 isoforms present in the rat adrenal gland. These activities were determined on immediately prepared adrenal microsomes following incubations with 3H-corticosterone and NAD + or NADP+. 11-dehydrocorticosterone was measured as an end-product by TLC, and controls were adrenal microsomes from rats kept under basal (unstressed) conditions. 11β-hydroxysteroid dehydrogenase 1 activity, but not 11β-hydroxysteroid dehydrogenase 2 activity, was increased under both stress-conditions. Homeostatically, the stimulation of 11β- hydroxysteroid dehydrogenase 1 activity would increase the supply of glucocorticoids. These, in turn, would activate the enzyme phenylethanolamine N-methyl transferase, thereby improving the synthesis of epinephrine as part of the stress-response. Fil:Zallocchi, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2004 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00084212_v82_n6_p422_Zallocchi http://hdl.handle.net/20.500.12110/paper_00084212_v82_n6_p422_Zallocchi
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Acidosis Adrenal HSD Stress 11beta hydroxysteroid dehydrogenase adrenalin corticosterone dehydrocorticosterone glucocorticoid hydrochloric acid isoenzyme nicotinamide adenine dinucleotide nicotinamide adenine dinucleotide phosphate phenylethanolamine n methyltransferase adrenal gland animal experiment animal tissue article controlled study enzyme activation enzyme activity experimental rat feeding homeostasis male measurement microsome nonhuman priority journal protein synthesis rat simulation stress thin layer chromatography
spellingShingle	Acidosis Adrenal HSD Stress 11beta hydroxysteroid dehydrogenase adrenalin corticosterone dehydrocorticosterone glucocorticoid hydrochloric acid isoenzyme nicotinamide adenine dinucleotide nicotinamide adenine dinucleotide phosphate phenylethanolamine n methyltransferase adrenal gland animal experiment animal tissue article controlled study enzyme activation enzyme activity experimental rat feeding homeostasis male measurement microsome nonhuman priority journal protein synthesis rat simulation stress thin layer chromatography Zallocchi, Marisa Laura Adrenal 11-beta hydroxysteroid dehydrogenase activity in response to stress
topic_facet	Acidosis Adrenal HSD Stress 11beta hydroxysteroid dehydrogenase adrenalin corticosterone dehydrocorticosterone glucocorticoid hydrochloric acid isoenzyme nicotinamide adenine dinucleotide nicotinamide adenine dinucleotide phosphate phenylethanolamine n methyltransferase adrenal gland animal experiment animal tissue article controlled study enzyme activation enzyme activity experimental rat feeding homeostasis male measurement microsome nonhuman priority journal protein synthesis rat simulation stress thin layer chromatography
description	This work studied the effect of stresses produced by simulated gavage or gavage with 200 mmol/L HCl two hours before adrenal extraction, on the activities of the 11β-hydroxysteroid dehydrogenase 1 and 11β-hydroxysteroid dehydrogenase 2 isoforms present in the rat adrenal gland. These activities were determined on immediately prepared adrenal microsomes following incubations with 3H-corticosterone and NAD + or NADP+. 11-dehydrocorticosterone was measured as an end-product by TLC, and controls were adrenal microsomes from rats kept under basal (unstressed) conditions. 11β-hydroxysteroid dehydrogenase 1 activity, but not 11β-hydroxysteroid dehydrogenase 2 activity, was increased under both stress-conditions. Homeostatically, the stimulation of 11β- hydroxysteroid dehydrogenase 1 activity would increase the supply of glucocorticoids. These, in turn, would activate the enzyme phenylethanolamine N-methyl transferase, thereby improving the synthesis of epinephrine as part of the stress-response.
author	Zallocchi, Marisa Laura
author_facet	Zallocchi, Marisa Laura
author_sort	Zallocchi, Marisa Laura
title	Adrenal 11-beta hydroxysteroid dehydrogenase activity in response to stress
title_short	Adrenal 11-beta hydroxysteroid dehydrogenase activity in response to stress
title_full	Adrenal 11-beta hydroxysteroid dehydrogenase activity in response to stress
title_fullStr	Adrenal 11-beta hydroxysteroid dehydrogenase activity in response to stress
title_full_unstemmed	Adrenal 11-beta hydroxysteroid dehydrogenase activity in response to stress
title_sort	adrenal 11-beta hydroxysteroid dehydrogenase activity in response to stress
publishDate	2004
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00084212_v82_n6_p422_Zallocchi http://hdl.handle.net/20.500.12110/paper_00084212_v82_n6_p422_Zallocchi
work_keys_str_mv	AT zallocchimarisalaura adrenal11betahydroxysteroiddehydrogenaseactivityinresponsetostress
_version_	1768545032720089088

Adrenal 11-beta hydroxysteroid dehydrogenase activity in response to stress

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