Arsenic-hypertolerant and arsenic-reducing bacteria isolated from wells in Tucumán, Argentina
Arsenic-hypertolerant bacteria were isolated from arsenic-contaminated well water from the village of Los Pereyra in Tucumán province, Argentina. Microorganisms that biotransform arsenic are a major factor in arsenic mobilization in contaminated aquifers. Groundwater analyses showed a level of arsen...
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2018
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paper:paper_00084166_v64_n11_p876_Maizel2023-06-08T14:32:00Z Arsenic-hypertolerant and arsenic-reducing bacteria isolated from wells in Tucumán, Argentina Arsenic contamination Arsenic-hypertolerant bacteria Arsenic-reducing bacteria Domestic water wells arsenic cadmium chromium copper ground water well water arsenic bacterial DNA drinking water anion aquifer pollution arsenate arsenic bacterium biotransformation concentration (composition) detection method drinking water microbial activity mobilization speciation (chemistry) well water Actinobacteria Alphaproteobacteria Argentina Article bacterial growth bacterial strain bacterium bacterium identification bacterium isolation Betaproteobacteria biotransformation Brevibacterium concentration (parameters) controlled study enrichment culture Gammaproteobacteria high performance liquid chromatography inductively coupled plasma mass spectrometry Microbacterium nonhuman nucleotide sequence phylogeny physical chemistry priority journal water analysis water contamination bacterium biotransformation environmental monitoring genetics isolation and purification metabolism microbiology polymerase chain reaction water pollutant Argentina Tucuman Actinobacteria Bacteria (microorganisms) Brevibacterium sp. Microbacterium sp. Argentina Arsenic Bacteria Biotransformation DNA, Bacterial Drinking Water Environmental Monitoring Groundwater Polymerase Chain Reaction Water Microbiology Water Pollutants, Chemical Arsenic-hypertolerant bacteria were isolated from arsenic-contaminated well water from the village of Los Pereyra in Tucumán province, Argentina. Microorganisms that biotransform arsenic are a major factor in arsenic mobilization in contaminated aquifers. Groundwater analyses showed a level of arsenic contamination (mean concentration of 978 μg·L−1) that exceeds the safe drinking water limit of 10 μg·L−1 recommended by the World Health Organization and the Argentine Food Code. There was considerable spatial variability in the concentration of arsenic in each of the wells analyzed and in the distribution of the major anions HCO3 –, SO4 2–, and Cl–. Eighteen bacterial strains were characterized. Six strains belonging to the Actinobacteria phylum were able to grow in media with 20 mmol·L–1 As(III) or 200 mmol·L–1 As(V) and were also highly resistant to Cr, Cd, and Cu. Their ability to biotransform arsenic was examined by speciation of the products by high-performance liquid chromatography inductively coupled plasma mass spectrometry. In addition, two strains, Brevibacterium sp. strain AE038-4 and Microbacterium sp. strain AE038-20, were capable of aerobic arsenate reduction, which suggests that these strains could increase the mobility of arsenic by formation of more mobile As(III). © 2018, Canadian Science Publishing. All rights reserved. 2018 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00084166_v64_n11_p876_Maizel http://hdl.handle.net/20.500.12110/paper_00084166_v64_n11_p876_Maizel |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
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R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Arsenic contamination Arsenic-hypertolerant bacteria Arsenic-reducing bacteria Domestic water wells arsenic cadmium chromium copper ground water well water arsenic bacterial DNA drinking water anion aquifer pollution arsenate arsenic bacterium biotransformation concentration (composition) detection method drinking water microbial activity mobilization speciation (chemistry) well water Actinobacteria Alphaproteobacteria Argentina Article bacterial growth bacterial strain bacterium bacterium identification bacterium isolation Betaproteobacteria biotransformation Brevibacterium concentration (parameters) controlled study enrichment culture Gammaproteobacteria high performance liquid chromatography inductively coupled plasma mass spectrometry Microbacterium nonhuman nucleotide sequence phylogeny physical chemistry priority journal water analysis water contamination bacterium biotransformation environmental monitoring genetics isolation and purification metabolism microbiology polymerase chain reaction water pollutant Argentina Tucuman Actinobacteria Bacteria (microorganisms) Brevibacterium sp. Microbacterium sp. Argentina Arsenic Bacteria Biotransformation DNA, Bacterial Drinking Water Environmental Monitoring Groundwater Polymerase Chain Reaction Water Microbiology Water Pollutants, Chemical |
| spellingShingle |
Arsenic contamination Arsenic-hypertolerant bacteria Arsenic-reducing bacteria Domestic water wells arsenic cadmium chromium copper ground water well water arsenic bacterial DNA drinking water anion aquifer pollution arsenate arsenic bacterium biotransformation concentration (composition) detection method drinking water microbial activity mobilization speciation (chemistry) well water Actinobacteria Alphaproteobacteria Argentina Article bacterial growth bacterial strain bacterium bacterium identification bacterium isolation Betaproteobacteria biotransformation Brevibacterium concentration (parameters) controlled study enrichment culture Gammaproteobacteria high performance liquid chromatography inductively coupled plasma mass spectrometry Microbacterium nonhuman nucleotide sequence phylogeny physical chemistry priority journal water analysis water contamination bacterium biotransformation environmental monitoring genetics isolation and purification metabolism microbiology polymerase chain reaction water pollutant Argentina Tucuman Actinobacteria Bacteria (microorganisms) Brevibacterium sp. Microbacterium sp. Argentina Arsenic Bacteria Biotransformation DNA, Bacterial Drinking Water Environmental Monitoring Groundwater Polymerase Chain Reaction Water Microbiology Water Pollutants, Chemical Arsenic-hypertolerant and arsenic-reducing bacteria isolated from wells in Tucumán, Argentina |
| topic_facet |
Arsenic contamination Arsenic-hypertolerant bacteria Arsenic-reducing bacteria Domestic water wells arsenic cadmium chromium copper ground water well water arsenic bacterial DNA drinking water anion aquifer pollution arsenate arsenic bacterium biotransformation concentration (composition) detection method drinking water microbial activity mobilization speciation (chemistry) well water Actinobacteria Alphaproteobacteria Argentina Article bacterial growth bacterial strain bacterium bacterium identification bacterium isolation Betaproteobacteria biotransformation Brevibacterium concentration (parameters) controlled study enrichment culture Gammaproteobacteria high performance liquid chromatography inductively coupled plasma mass spectrometry Microbacterium nonhuman nucleotide sequence phylogeny physical chemistry priority journal water analysis water contamination bacterium biotransformation environmental monitoring genetics isolation and purification metabolism microbiology polymerase chain reaction water pollutant Argentina Tucuman Actinobacteria Bacteria (microorganisms) Brevibacterium sp. Microbacterium sp. Argentina Arsenic Bacteria Biotransformation DNA, Bacterial Drinking Water Environmental Monitoring Groundwater Polymerase Chain Reaction Water Microbiology Water Pollutants, Chemical |
| description |
Arsenic-hypertolerant bacteria were isolated from arsenic-contaminated well water from the village of Los Pereyra in Tucumán province, Argentina. Microorganisms that biotransform arsenic are a major factor in arsenic mobilization in contaminated aquifers. Groundwater analyses showed a level of arsenic contamination (mean concentration of 978 μg·L−1) that exceeds the safe drinking water limit of 10 μg·L−1 recommended by the World Health Organization and the Argentine Food Code. There was considerable spatial variability in the concentration of arsenic in each of the wells analyzed and in the distribution of the major anions HCO3 –, SO4 2–, and Cl–. Eighteen bacterial strains were characterized. Six strains belonging to the Actinobacteria phylum were able to grow in media with 20 mmol·L–1 As(III) or 200 mmol·L–1 As(V) and were also highly resistant to Cr, Cd, and Cu. Their ability to biotransform arsenic was examined by speciation of the products by high-performance liquid chromatography inductively coupled plasma mass spectrometry. In addition, two strains, Brevibacterium sp. strain AE038-4 and Microbacterium sp. strain AE038-20, were capable of aerobic arsenate reduction, which suggests that these strains could increase the mobility of arsenic by formation of more mobile As(III). © 2018, Canadian Science Publishing. All rights reserved. |
| title |
Arsenic-hypertolerant and arsenic-reducing bacteria isolated from wells in Tucumán, Argentina |
| title_short |
Arsenic-hypertolerant and arsenic-reducing bacteria isolated from wells in Tucumán, Argentina |
| title_full |
Arsenic-hypertolerant and arsenic-reducing bacteria isolated from wells in Tucumán, Argentina |
| title_fullStr |
Arsenic-hypertolerant and arsenic-reducing bacteria isolated from wells in Tucumán, Argentina |
| title_full_unstemmed |
Arsenic-hypertolerant and arsenic-reducing bacteria isolated from wells in Tucumán, Argentina |
| title_sort |
arsenic-hypertolerant and arsenic-reducing bacteria isolated from wells in tucumán, argentina |
| publishDate |
2018 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00084166_v64_n11_p876_Maizel http://hdl.handle.net/20.500.12110/paper_00084166_v64_n11_p876_Maizel |
| _version_ |
1768543305557082112 |