Studies on uroporphyrinogen decarboxylase from Chlorella kessleri (Trebouxiophyceae, Chlorophyta)

Uroporphyrinogen decarboxylase (UroD) (EC 4.1.1.37) is an enzyme from the tetrapyrrole biosynthetic pathway, in which chlorophyll is the main final product in algae. This is the first time that a study on UroD activity has been performed in a green alga (Chlorella). We isolated and partially purifie...

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Autores principales:	Juárez, Angela Beatriz, Vigna, María Susana, Ríos de Molina, María del Carmen
Publicado:	2007
Materias:	Chlorella kessleri Chlorophyll Chlorophyta Porphyrins Uroporphyrinogen decarboxylase Bioassay Biosynthesis Chromatographic analysis Molecular mass Reaction kinetics Biosynthetic pathway Decarboxylation Enzyme kinetics chlorophyll uroporphyrinogen decarboxylase alga bioassay decarboxylation enzyme activity growth rate porphyrin article Chlorella controlled study enzyme purification gel filtration green alga incubation time nonhuman priority journal Western blotting Molecular Weight Uroporphyrinogen Decarboxylase algae Chlorella (Chlorellales) Chlorella (Prasiolales) Chlorella (unclassified Chlorophyceae) Chlorella (unclassified Trebouxiophyceae) Parachlorella kessleri Trebouxiophyceae
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00084166_v53_n2_p303_Juarez http://hdl.handle.net/20.500.12110/paper_00084166_v53_n2_p303_Juarez
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00084166_v53_n2_p303_Juarez
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spelling	paper:paper_00084166_v53_n2_p303_Juarez2023-06-08T14:31:59Z Studies on uroporphyrinogen decarboxylase from Chlorella kessleri (Trebouxiophyceae, Chlorophyta) Juárez, Angela Beatriz Vigna, María Susana Ríos de Molina, María del Carmen Chlorella kessleri Chlorophyll Chlorophyta Porphyrins Uroporphyrinogen decarboxylase Bioassay Biosynthesis Chlorophyll Chromatographic analysis Molecular mass Porphyrins Reaction kinetics Biosynthetic pathway Chlorella kessleri Chlorophyta Decarboxylation Enzyme kinetics chlorophyll uroporphyrinogen decarboxylase alga bioassay decarboxylation enzyme activity growth rate porphyrin article Chlorella controlled study decarboxylation enzyme activity enzyme purification gel filtration green alga incubation time nonhuman priority journal Western blotting Chlorella Molecular Weight Uroporphyrinogen Decarboxylase algae Chlorella (Chlorellales) Chlorella (Prasiolales) Chlorella (unclassified Chlorophyceae) Chlorella (unclassified Trebouxiophyceae) Chlorophyta Parachlorella kessleri Trebouxiophyceae Uroporphyrinogen decarboxylase (UroD) (EC 4.1.1.37) is an enzyme from the tetrapyrrole biosynthetic pathway, in which chlorophyll is the main final product in algae. This is the first time that a study on UroD activity has been performed in a green alga (Chlorella). We isolated and partially purified the enzyme from a Chlorella kessleri (Trebouxiophyceae, Chlorophyta) strain (Copahue, Neuquen, Argentina), and describe for the first time some of its properties. In C. kessleri, the decarboxylation of uroporphyrinogen III occurs in two stages, via 7 COOH and then 6 and 5 COOH intermediates, with the decarboxylation of the 7 COOH compound being the rate-limiting step for the reaction. Cultures in the exponential growth phase showed the highest specific activity values. The most suitable conditions to measure UroD activity in C. kessleri were as follows: 0.23-0.3 mg protein/mL, ≈6-8 μmol/L uroporphyrinogen III, and 20 min incubation time. Gel filtration chromatography and Western blot assays indicated that UroD from C. kessleri is a dimer of approximately 90 kDa formed by species of lower molecular mass, which conserves enzymatic activity. © 2007 NRC. Fil:Juárez, A.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Vigna, M.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ríos De Molina, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2007 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00084166_v53_n2_p303_Juarez http://hdl.handle.net/20.500.12110/paper_00084166_v53_n2_p303_Juarez
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Chlorella kessleri Chlorophyll Chlorophyta Porphyrins Uroporphyrinogen decarboxylase Bioassay Biosynthesis Chlorophyll Chromatographic analysis Molecular mass Porphyrins Reaction kinetics Biosynthetic pathway Chlorella kessleri Chlorophyta Decarboxylation Enzyme kinetics chlorophyll uroporphyrinogen decarboxylase alga bioassay decarboxylation enzyme activity growth rate porphyrin article Chlorella controlled study decarboxylation enzyme activity enzyme purification gel filtration green alga incubation time nonhuman priority journal Western blotting Chlorella Molecular Weight Uroporphyrinogen Decarboxylase algae Chlorella (Chlorellales) Chlorella (Prasiolales) Chlorella (unclassified Chlorophyceae) Chlorella (unclassified Trebouxiophyceae) Chlorophyta Parachlorella kessleri Trebouxiophyceae
spellingShingle	Chlorella kessleri Chlorophyll Chlorophyta Porphyrins Uroporphyrinogen decarboxylase Bioassay Biosynthesis Chlorophyll Chromatographic analysis Molecular mass Porphyrins Reaction kinetics Biosynthetic pathway Chlorella kessleri Chlorophyta Decarboxylation Enzyme kinetics chlorophyll uroporphyrinogen decarboxylase alga bioassay decarboxylation enzyme activity growth rate porphyrin article Chlorella controlled study decarboxylation enzyme activity enzyme purification gel filtration green alga incubation time nonhuman priority journal Western blotting Chlorella Molecular Weight Uroporphyrinogen Decarboxylase algae Chlorella (Chlorellales) Chlorella (Prasiolales) Chlorella (unclassified Chlorophyceae) Chlorella (unclassified Trebouxiophyceae) Chlorophyta Parachlorella kessleri Trebouxiophyceae Juárez, Angela Beatriz Vigna, María Susana Ríos de Molina, María del Carmen Studies on uroporphyrinogen decarboxylase from Chlorella kessleri (Trebouxiophyceae, Chlorophyta)
topic_facet	Chlorella kessleri Chlorophyll Chlorophyta Porphyrins Uroporphyrinogen decarboxylase Bioassay Biosynthesis Chlorophyll Chromatographic analysis Molecular mass Porphyrins Reaction kinetics Biosynthetic pathway Chlorella kessleri Chlorophyta Decarboxylation Enzyme kinetics chlorophyll uroporphyrinogen decarboxylase alga bioassay decarboxylation enzyme activity growth rate porphyrin article Chlorella controlled study decarboxylation enzyme activity enzyme purification gel filtration green alga incubation time nonhuman priority journal Western blotting Chlorella Molecular Weight Uroporphyrinogen Decarboxylase algae Chlorella (Chlorellales) Chlorella (Prasiolales) Chlorella (unclassified Chlorophyceae) Chlorella (unclassified Trebouxiophyceae) Chlorophyta Parachlorella kessleri Trebouxiophyceae
description	Uroporphyrinogen decarboxylase (UroD) (EC 4.1.1.37) is an enzyme from the tetrapyrrole biosynthetic pathway, in which chlorophyll is the main final product in algae. This is the first time that a study on UroD activity has been performed in a green alga (Chlorella). We isolated and partially purified the enzyme from a Chlorella kessleri (Trebouxiophyceae, Chlorophyta) strain (Copahue, Neuquen, Argentina), and describe for the first time some of its properties. In C. kessleri, the decarboxylation of uroporphyrinogen III occurs in two stages, via 7 COOH and then 6 and 5 COOH intermediates, with the decarboxylation of the 7 COOH compound being the rate-limiting step for the reaction. Cultures in the exponential growth phase showed the highest specific activity values. The most suitable conditions to measure UroD activity in C. kessleri were as follows: 0.23-0.3 mg protein/mL, ≈6-8 μmol/L uroporphyrinogen III, and 20 min incubation time. Gel filtration chromatography and Western blot assays indicated that UroD from C. kessleri is a dimer of approximately 90 kDa formed by species of lower molecular mass, which conserves enzymatic activity. © 2007 NRC.
author	Juárez, Angela Beatriz Vigna, María Susana Ríos de Molina, María del Carmen
author_facet	Juárez, Angela Beatriz Vigna, María Susana Ríos de Molina, María del Carmen
author_sort	Juárez, Angela Beatriz
title	Studies on uroporphyrinogen decarboxylase from Chlorella kessleri (Trebouxiophyceae, Chlorophyta)
title_short	Studies on uroporphyrinogen decarboxylase from Chlorella kessleri (Trebouxiophyceae, Chlorophyta)
title_full	Studies on uroporphyrinogen decarboxylase from Chlorella kessleri (Trebouxiophyceae, Chlorophyta)
title_fullStr	Studies on uroporphyrinogen decarboxylase from Chlorella kessleri (Trebouxiophyceae, Chlorophyta)
title_full_unstemmed	Studies on uroporphyrinogen decarboxylase from Chlorella kessleri (Trebouxiophyceae, Chlorophyta)
title_sort	studies on uroporphyrinogen decarboxylase from chlorella kessleri (trebouxiophyceae, chlorophyta)
publishDate	2007
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00084166_v53_n2_p303_Juarez http://hdl.handle.net/20.500.12110/paper_00084166_v53_n2_p303_Juarez
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Studies on uroporphyrinogen decarboxylase from Chlorella kessleri (Trebouxiophyceae, Chlorophyta)

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