Physiological and genetic characterization of the osmotic stress response in Bacillus subtilis
Bacillus subtilis cultures submitted to an osmotic upshock (1.5 M NaCl) lysed unless stationary phase had been reached. Several physiological variations were observed, such as delayed growth (adaptation), a filamentous bacterial appearance, RecA-dependent osmoresistance (SOS), and cross-induction by...
Guardado en:
| Publicado: |
1994
|
|---|---|
| Materias: | |
| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00084166_v40_n2_p140_Ruzal http://hdl.handle.net/20.500.12110/paper_00084166_v40_n2_p140_Ruzal |
| Aporte de: |
| id |
paper:paper_00084166_v40_n2_p140_Ruzal |
|---|---|
| record_format |
dspace |
| spelling |
paper:paper_00084166_v40_n2_p140_Ruzal2023-06-08T14:31:59Z Physiological and genetic characterization of the osmotic stress response in Bacillus subtilis Bacillus subtilis osmotic stress sporulation adaptation article Bacillus subtilis bacterial growth bacterium mutant nonhuman osmotic stress priority journal sporogenesis Bacillus subtilis Bacteria (microorganisms) Octodon degus Bacillus subtilis cultures submitted to an osmotic upshock (1.5 M NaCl) lysed unless stationary phase had been reached. Several physiological variations were observed, such as delayed growth (adaptation), a filamentous bacterial appearance, RecA-dependent osmoresistance (SOS), and cross-induction by a previous stress (heat shock). Osmoresistance and sporulation seem to share pathways of regulation such as inhibition in the presence of glucose and glutamine and derepression in a catabolite-resistant mutant such as degUh. However, spores were not obtained on hypertonic media. Mutants of later sporulation stages (spoII, spoIII) presented a response similar to that of the wild-type parent, indicating that both processes probably shared early controls. Null mutations in any of the known key modulators of sporulation (spoOA or degU) resulted in similar levels of osmosensitivity. Sensor mutations in kinA and degS also led to strains with altered responses, the kinA mutant being even more osmosensitive than the degS mutant. Several spoOA mutant phenotypes are due to this gene's control of abrB, a regulator of stationary-phase events, and an abrB mutation relieved the osmosensitivity of the spoOA-containing mutant but had no effect on a wild-tytpe strain. 1994 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00084166_v40_n2_p140_Ruzal http://hdl.handle.net/20.500.12110/paper_00084166_v40_n2_p140_Ruzal |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Bacillus subtilis osmotic stress sporulation adaptation article Bacillus subtilis bacterial growth bacterium mutant nonhuman osmotic stress priority journal sporogenesis Bacillus subtilis Bacteria (microorganisms) Octodon degus |
| spellingShingle |
Bacillus subtilis osmotic stress sporulation adaptation article Bacillus subtilis bacterial growth bacterium mutant nonhuman osmotic stress priority journal sporogenesis Bacillus subtilis Bacteria (microorganisms) Octodon degus Physiological and genetic characterization of the osmotic stress response in Bacillus subtilis |
| topic_facet |
Bacillus subtilis osmotic stress sporulation adaptation article Bacillus subtilis bacterial growth bacterium mutant nonhuman osmotic stress priority journal sporogenesis Bacillus subtilis Bacteria (microorganisms) Octodon degus |
| description |
Bacillus subtilis cultures submitted to an osmotic upshock (1.5 M NaCl) lysed unless stationary phase had been reached. Several physiological variations were observed, such as delayed growth (adaptation), a filamentous bacterial appearance, RecA-dependent osmoresistance (SOS), and cross-induction by a previous stress (heat shock). Osmoresistance and sporulation seem to share pathways of regulation such as inhibition in the presence of glucose and glutamine and derepression in a catabolite-resistant mutant such as degUh. However, spores were not obtained on hypertonic media. Mutants of later sporulation stages (spoII, spoIII) presented a response similar to that of the wild-type parent, indicating that both processes probably shared early controls. Null mutations in any of the known key modulators of sporulation (spoOA or degU) resulted in similar levels of osmosensitivity. Sensor mutations in kinA and degS also led to strains with altered responses, the kinA mutant being even more osmosensitive than the degS mutant. Several spoOA mutant phenotypes are due to this gene's control of abrB, a regulator of stationary-phase events, and an abrB mutation relieved the osmosensitivity of the spoOA-containing mutant but had no effect on a wild-tytpe strain. |
| title |
Physiological and genetic characterization of the osmotic stress response in Bacillus subtilis |
| title_short |
Physiological and genetic characterization of the osmotic stress response in Bacillus subtilis |
| title_full |
Physiological and genetic characterization of the osmotic stress response in Bacillus subtilis |
| title_fullStr |
Physiological and genetic characterization of the osmotic stress response in Bacillus subtilis |
| title_full_unstemmed |
Physiological and genetic characterization of the osmotic stress response in Bacillus subtilis |
| title_sort |
physiological and genetic characterization of the osmotic stress response in bacillus subtilis |
| publishDate |
1994 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00084166_v40_n2_p140_Ruzal http://hdl.handle.net/20.500.12110/paper_00084166_v40_n2_p140_Ruzal |
| _version_ |
1768546049485438976 |