Nuclear translocation of haeme oxygenase-1 is associated to prostate cancer

The role of oxidative stress in prostate cancer has been increasingly recognised. Acute and chronic inflammations generate reactive oxygen species that result in damage to cellular structures. Haeme oxygenase-1 (HO-1) has cytoprotective effects against oxidative damage. We hypothesise that modulatio...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Sacca, Paula Alejandra, Calvo, Juan Carlos, Vázquez, Elba Susana
Publicado:	2007
Materias:	Haeme oxygenase-1 Nuclear localisation Oxidative stress Prostate cancer heme oxygenase 1 hemin adult aged article cancer cell culture cancer growth carcinogenesis cell compartmentalization cell nucleus cell protection controlled study disease association disease course enzyme localization enzyme regulation Gleason score human human cell human tissue immunohistochemistry male oxidative stress priority journal prostate cancer prostate hypertrophy prostatectomy protein expression Western blotting Active Transport, Cell Nucleus Adult Aged Aged, 80 and over Cell Nucleus Heme Oxygenase-1 Hemin Humans Male Middle Aged Prostatic Hyperplasia Prostatic Neoplasms Tumor Cells, Cultured
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00070920_v97_n12_p1683_Sacca http://hdl.handle.net/20.500.12110/paper_00070920_v97_n12_p1683_Sacca
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00070920_v97_n12_p1683_Sacca
record_format	dspace
spelling	paper:paper_00070920_v97_n12_p1683_Sacca2023-06-08T14:31:35Z Nuclear translocation of haeme oxygenase-1 is associated to prostate cancer Sacca, Paula Alejandra Calvo, Juan Carlos Vázquez, Elba Susana Haeme oxygenase-1 Nuclear localisation Oxidative stress Prostate cancer heme oxygenase 1 hemin adult aged article cancer cell culture cancer growth carcinogenesis cell compartmentalization cell nucleus cell protection controlled study disease association disease course enzyme localization enzyme regulation Gleason score human human cell human tissue immunohistochemistry male oxidative stress priority journal prostate cancer prostate hypertrophy prostatectomy protein expression Western blotting Active Transport, Cell Nucleus Adult Aged Aged, 80 and over Cell Nucleus Heme Oxygenase-1 Hemin Humans Male Middle Aged Prostatic Hyperplasia Prostatic Neoplasms Tumor Cells, Cultured The role of oxidative stress in prostate cancer has been increasingly recognised. Acute and chronic inflammations generate reactive oxygen species that result in damage to cellular structures. Haeme oxygenase-1 (HO-1) has cytoprotective effects against oxidative damage. We hypothesise that modulation of HO-1 expression may be involved in the process of prostate carcinogenesis and prostate cancer progression. We thus studied HO-1 expression and localisation in 85 samples of organ-confined primary prostate cancer obtained via radical prostatectomy (Gleason grades 4-9) and in 39 specimens of benign prostatic hyperplasia (BPH). We assessed HO-1 expression by immunohistochemical staining. No significant difference was observed in the cytoplasmic positive reactivity among tumours (84%), non-neoplastic surrounding parenchyma (89%), or BPH samples (87%) (P=0.53). Haeme oxygenase-1 immunostaining was detected in the nuclei of prostate cancer cells in 55 of 85 (65%) patients but less often in non-neoplastic surrounding parenchyma (30 of 85, 35%) or in BPH (9 of 39, 23%) (P<0.0001). Immunocytochemical and western blot analysis showed HO-1 only in the cytoplasmic compartment of PC3 and LNCaP prostate cancer cell lines. Treatment with hemin, a well-known specific inducer of HO-1, led to clear nuclear localisation of HO-1 in both cell lines and highly induced HO-1 expression in both cellular compartments. These findings have demonstrated, for the first time, that HO-1 expression and nuclear localisation can define a new subgroup of prostate cancer primary tumours and that the modulation of HO-1 expression and its nuclear translocation could represent new avenues for therapy. © 2007 Cancer Research UK. Fil:Sacca, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Calvo, J.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Vazquez, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2007 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00070920_v97_n12_p1683_Sacca http://hdl.handle.net/20.500.12110/paper_00070920_v97_n12_p1683_Sacca
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Haeme oxygenase-1 Nuclear localisation Oxidative stress Prostate cancer heme oxygenase 1 hemin adult aged article cancer cell culture cancer growth carcinogenesis cell compartmentalization cell nucleus cell protection controlled study disease association disease course enzyme localization enzyme regulation Gleason score human human cell human tissue immunohistochemistry male oxidative stress priority journal prostate cancer prostate hypertrophy prostatectomy protein expression Western blotting Active Transport, Cell Nucleus Adult Aged Aged, 80 and over Cell Nucleus Heme Oxygenase-1 Hemin Humans Male Middle Aged Prostatic Hyperplasia Prostatic Neoplasms Tumor Cells, Cultured
spellingShingle	Haeme oxygenase-1 Nuclear localisation Oxidative stress Prostate cancer heme oxygenase 1 hemin adult aged article cancer cell culture cancer growth carcinogenesis cell compartmentalization cell nucleus cell protection controlled study disease association disease course enzyme localization enzyme regulation Gleason score human human cell human tissue immunohistochemistry male oxidative stress priority journal prostate cancer prostate hypertrophy prostatectomy protein expression Western blotting Active Transport, Cell Nucleus Adult Aged Aged, 80 and over Cell Nucleus Heme Oxygenase-1 Hemin Humans Male Middle Aged Prostatic Hyperplasia Prostatic Neoplasms Tumor Cells, Cultured Sacca, Paula Alejandra Calvo, Juan Carlos Vázquez, Elba Susana Nuclear translocation of haeme oxygenase-1 is associated to prostate cancer
topic_facet	Haeme oxygenase-1 Nuclear localisation Oxidative stress Prostate cancer heme oxygenase 1 hemin adult aged article cancer cell culture cancer growth carcinogenesis cell compartmentalization cell nucleus cell protection controlled study disease association disease course enzyme localization enzyme regulation Gleason score human human cell human tissue immunohistochemistry male oxidative stress priority journal prostate cancer prostate hypertrophy prostatectomy protein expression Western blotting Active Transport, Cell Nucleus Adult Aged Aged, 80 and over Cell Nucleus Heme Oxygenase-1 Hemin Humans Male Middle Aged Prostatic Hyperplasia Prostatic Neoplasms Tumor Cells, Cultured
description	The role of oxidative stress in prostate cancer has been increasingly recognised. Acute and chronic inflammations generate reactive oxygen species that result in damage to cellular structures. Haeme oxygenase-1 (HO-1) has cytoprotective effects against oxidative damage. We hypothesise that modulation of HO-1 expression may be involved in the process of prostate carcinogenesis and prostate cancer progression. We thus studied HO-1 expression and localisation in 85 samples of organ-confined primary prostate cancer obtained via radical prostatectomy (Gleason grades 4-9) and in 39 specimens of benign prostatic hyperplasia (BPH). We assessed HO-1 expression by immunohistochemical staining. No significant difference was observed in the cytoplasmic positive reactivity among tumours (84%), non-neoplastic surrounding parenchyma (89%), or BPH samples (87%) (P=0.53). Haeme oxygenase-1 immunostaining was detected in the nuclei of prostate cancer cells in 55 of 85 (65%) patients but less often in non-neoplastic surrounding parenchyma (30 of 85, 35%) or in BPH (9 of 39, 23%) (P<0.0001). Immunocytochemical and western blot analysis showed HO-1 only in the cytoplasmic compartment of PC3 and LNCaP prostate cancer cell lines. Treatment with hemin, a well-known specific inducer of HO-1, led to clear nuclear localisation of HO-1 in both cell lines and highly induced HO-1 expression in both cellular compartments. These findings have demonstrated, for the first time, that HO-1 expression and nuclear localisation can define a new subgroup of prostate cancer primary tumours and that the modulation of HO-1 expression and its nuclear translocation could represent new avenues for therapy. © 2007 Cancer Research UK.
author	Sacca, Paula Alejandra Calvo, Juan Carlos Vázquez, Elba Susana
author_facet	Sacca, Paula Alejandra Calvo, Juan Carlos Vázquez, Elba Susana
author_sort	Sacca, Paula Alejandra
title	Nuclear translocation of haeme oxygenase-1 is associated to prostate cancer
title_short	Nuclear translocation of haeme oxygenase-1 is associated to prostate cancer
title_full	Nuclear translocation of haeme oxygenase-1 is associated to prostate cancer
title_fullStr	Nuclear translocation of haeme oxygenase-1 is associated to prostate cancer
title_full_unstemmed	Nuclear translocation of haeme oxygenase-1 is associated to prostate cancer
title_sort	nuclear translocation of haeme oxygenase-1 is associated to prostate cancer
publishDate	2007
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00070920_v97_n12_p1683_Sacca http://hdl.handle.net/20.500.12110/paper_00070920_v97_n12_p1683_Sacca
work_keys_str_mv	AT saccapaulaalejandra nucleartranslocationofhaemeoxygenase1isassociatedtoprostatecancer AT calvojuancarlos nucleartranslocationofhaemeoxygenase1isassociatedtoprostatecancer AT vazquezelbasusana nucleartranslocationofhaemeoxygenase1isassociatedtoprostatecancer
_version_	1768546609937776640

Nuclear translocation of haeme oxygenase-1 is associated to prostate cancer

Ejemplares similares