Mechanistic studies on δ-aminolevulinic acid uptake and efflux in a mammary adenocarcinoma cell line

δ-aminolevulinic acid (ALA) is the precursor in the biosynthesis of porphyrins. The knowledge of both the regulation of ALA entrance and efflux from the cells and the control of porphyrin biosynthesis is essential to improve ALA-mediated photodynamic therapy. In this work, we studied the regulation...

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Autores principales:	Correa García, Susana Raquel, Casas, Adriana Gabriela, Perotti, Christian Pablo, Batlle, Alcira María del Carmen, Bermudez Moretti, Mariana
Publicado:	2003
Materias:	δ-aminolevulinic acid ALA efflux ALA uptake Photodynamic therapy 4 aminobutyric acid aminolevulinic acid heme derivative porphobilinogen porphyrin succinylacetone animal cell article breast adenocarcinoma cancer cell culture cell transport controlled study diffusion heme synthesis mouse nonhuman photodynamic therapy priority journal protein function protein protein interaction protein transport temperature dependence Adenocarcinoma Aminolevulinic Acid Animals gamma-Aminobutyric Acid Mammary Neoplasms, Animal Mice Photochemotherapy Photosensitizing Agents Porphyrins Tumor Cells, Cultured
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00070920_v89_n1_p173_CorreaGarcia http://hdl.handle.net/20.500.12110/paper_00070920_v89_n1_p173_CorreaGarcia
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_00070920_v89_n1_p173_CorreaGarcia
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spelling	paper:paper_00070920_v89_n1_p173_CorreaGarcia2023-06-08T14:31:34Z Mechanistic studies on δ-aminolevulinic acid uptake and efflux in a mammary adenocarcinoma cell line Correa García, Susana Raquel Casas, Adriana Gabriela Perotti, Christian Pablo Batlle, Alcira María del Carmen Bermudez Moretti, Mariana δ-aminolevulinic acid ALA efflux ALA uptake Photodynamic therapy 4 aminobutyric acid aminolevulinic acid heme derivative porphobilinogen porphyrin succinylacetone animal cell article breast adenocarcinoma cancer cell culture cell transport controlled study diffusion heme synthesis mouse nonhuman photodynamic therapy priority journal protein function protein protein interaction protein transport temperature dependence Adenocarcinoma Aminolevulinic Acid Animals gamma-Aminobutyric Acid Mammary Neoplasms, Animal Mice Photochemotherapy Photosensitizing Agents Porphyrins Tumor Cells, Cultured δ-aminolevulinic acid (ALA) is the precursor in the biosynthesis of porphyrins. The knowledge of both the regulation of ALA entrance and efflux from the cells and the control of porphyrin biosynthesis is essential to improve ALA-mediated photodynamic therapy. In this work, we studied the regulation of ALA uptake and efflux by endogenously accumulated ALA and/or porphyrins in murine mammary adenocarcinoma cells. Under our set of conditions, the haem synthesis inhibitor succinyl acetone completely prevented porphobilinogen and porphyrin synthesis from ALA, and led to an increase in the intracellular ALA pool. However, neither intracellular ALA nor porphyrin pools regulate ALA uptake or efflux during the first 15 min of the process. Based on temperature dependence data, ALA but not γ-aminobutyric acid (GABA) efflux is mediated by a diffusion mechanism. Moreover, the addition of extracellular GABA not only did not influence the rate of ALA efflux but on the contrary it affected ALA uptake, showing the contribution of a saturable mechanism for the uptake, but not for the efflux of ALA from the cells. © 2003 Cancer Research UK. Fil:Correa García, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Casas, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Perotti, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Batlle, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bermúdez Moretti, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2003 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00070920_v89_n1_p173_CorreaGarcia http://hdl.handle.net/20.500.12110/paper_00070920_v89_n1_p173_CorreaGarcia
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	δ-aminolevulinic acid ALA efflux ALA uptake Photodynamic therapy 4 aminobutyric acid aminolevulinic acid heme derivative porphobilinogen porphyrin succinylacetone animal cell article breast adenocarcinoma cancer cell culture cell transport controlled study diffusion heme synthesis mouse nonhuman photodynamic therapy priority journal protein function protein protein interaction protein transport temperature dependence Adenocarcinoma Aminolevulinic Acid Animals gamma-Aminobutyric Acid Mammary Neoplasms, Animal Mice Photochemotherapy Photosensitizing Agents Porphyrins Tumor Cells, Cultured
spellingShingle	δ-aminolevulinic acid ALA efflux ALA uptake Photodynamic therapy 4 aminobutyric acid aminolevulinic acid heme derivative porphobilinogen porphyrin succinylacetone animal cell article breast adenocarcinoma cancer cell culture cell transport controlled study diffusion heme synthesis mouse nonhuman photodynamic therapy priority journal protein function protein protein interaction protein transport temperature dependence Adenocarcinoma Aminolevulinic Acid Animals gamma-Aminobutyric Acid Mammary Neoplasms, Animal Mice Photochemotherapy Photosensitizing Agents Porphyrins Tumor Cells, Cultured Correa García, Susana Raquel Casas, Adriana Gabriela Perotti, Christian Pablo Batlle, Alcira María del Carmen Bermudez Moretti, Mariana Mechanistic studies on δ-aminolevulinic acid uptake and efflux in a mammary adenocarcinoma cell line
topic_facet	δ-aminolevulinic acid ALA efflux ALA uptake Photodynamic therapy 4 aminobutyric acid aminolevulinic acid heme derivative porphobilinogen porphyrin succinylacetone animal cell article breast adenocarcinoma cancer cell culture cell transport controlled study diffusion heme synthesis mouse nonhuman photodynamic therapy priority journal protein function protein protein interaction protein transport temperature dependence Adenocarcinoma Aminolevulinic Acid Animals gamma-Aminobutyric Acid Mammary Neoplasms, Animal Mice Photochemotherapy Photosensitizing Agents Porphyrins Tumor Cells, Cultured
description	δ-aminolevulinic acid (ALA) is the precursor in the biosynthesis of porphyrins. The knowledge of both the regulation of ALA entrance and efflux from the cells and the control of porphyrin biosynthesis is essential to improve ALA-mediated photodynamic therapy. In this work, we studied the regulation of ALA uptake and efflux by endogenously accumulated ALA and/or porphyrins in murine mammary adenocarcinoma cells. Under our set of conditions, the haem synthesis inhibitor succinyl acetone completely prevented porphobilinogen and porphyrin synthesis from ALA, and led to an increase in the intracellular ALA pool. However, neither intracellular ALA nor porphyrin pools regulate ALA uptake or efflux during the first 15 min of the process. Based on temperature dependence data, ALA but not γ-aminobutyric acid (GABA) efflux is mediated by a diffusion mechanism. Moreover, the addition of extracellular GABA not only did not influence the rate of ALA efflux but on the contrary it affected ALA uptake, showing the contribution of a saturable mechanism for the uptake, but not for the efflux of ALA from the cells. © 2003 Cancer Research UK.
author	Correa García, Susana Raquel Casas, Adriana Gabriela Perotti, Christian Pablo Batlle, Alcira María del Carmen Bermudez Moretti, Mariana
author_facet	Correa García, Susana Raquel Casas, Adriana Gabriela Perotti, Christian Pablo Batlle, Alcira María del Carmen Bermudez Moretti, Mariana
author_sort	Correa García, Susana Raquel
title	Mechanistic studies on δ-aminolevulinic acid uptake and efflux in a mammary adenocarcinoma cell line
title_short	Mechanistic studies on δ-aminolevulinic acid uptake and efflux in a mammary adenocarcinoma cell line
title_full	Mechanistic studies on δ-aminolevulinic acid uptake and efflux in a mammary adenocarcinoma cell line
title_fullStr	Mechanistic studies on δ-aminolevulinic acid uptake and efflux in a mammary adenocarcinoma cell line
title_full_unstemmed	Mechanistic studies on δ-aminolevulinic acid uptake and efflux in a mammary adenocarcinoma cell line
title_sort	mechanistic studies on δ-aminolevulinic acid uptake and efflux in a mammary adenocarcinoma cell line
publishDate	2003
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00070920_v89_n1_p173_CorreaGarcia http://hdl.handle.net/20.500.12110/paper_00070920_v89_n1_p173_CorreaGarcia
work_keys_str_mv	AT correagarciasusanaraquel mechanisticstudiesondaminolevulinicaciduptakeandeffluxinamammaryadenocarcinomacellline AT casasadrianagabriela mechanisticstudiesondaminolevulinicaciduptakeandeffluxinamammaryadenocarcinomacellline AT perottichristianpablo mechanisticstudiesondaminolevulinicaciduptakeandeffluxinamammaryadenocarcinomacellline AT batllealciramariadelcarmen mechanisticstudiesondaminolevulinicaciduptakeandeffluxinamammaryadenocarcinomacellline AT bermudezmorettimariana mechanisticstudiesondaminolevulinicaciduptakeandeffluxinamammaryadenocarcinomacellline
_version_	1768546424477188096

Mechanistic studies on δ-aminolevulinic acid uptake and efflux in a mammary adenocarcinoma cell line

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