δ-aminolevulinic acid transport in murine mammary adenocarcinoma cells is mediated by beta transporters
δ-aminolevulinic acid, the precursor of porphyrin biosynthesis has been used to induce the endogenous synthesis of the photosensitiser protoporphyrin IX for photodynamic therapy in the treatment of various tumours. The aim of this work was to characterise the δ-aminolevulinic acid transport system i...
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2002
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00070920_v87_n4_p471_BermudezMoretti http://hdl.handle.net/20.500.12110/paper_00070920_v87_n4_p471_BermudezMoretti |
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paper:paper_00070920_v87_n4_p471_BermudezMoretti2023-06-08T14:31:34Z δ-aminolevulinic acid transport in murine mammary adenocarcinoma cells is mediated by beta transporters Bermudez Moretti, Mariana Correa García, Susana Raquel Perotti, Christian Pablo Batlle, Alcira María del Carmen Casas, Adriana Gabriela BETA transporters Membrane transport Photodynamic therapy δ-aminolevulinic acid aminolevulinic acid carrier protein animal cell animal model article breast adenocarcinoma cancer cell culture cell membrane transport controlled study diffusion drug transport drug uptake mouse nonhuman priority journal transport kinetics Adenocarcinoma Aminolevulinic Acid Animals Biological Transport, Active Mammary Neoplasms, Experimental Mice Tumor Cells, Cultured δ-aminolevulinic acid, the precursor of porphyrin biosynthesis has been used to induce the endogenous synthesis of the photosensitiser protoporphyrin IX for photodynamic therapy in the treatment of various tumours. The aim of this work was to characterise the δ-aminolevulinic acid transport system in the murine mammary adenocarcinoma cell line LM3 using 14C-δ-aminolevulinic acid, to finally improve δ-aminolevulinic acid incorporation in mammalian cells. Our results showed that δ aminolevulinic acid is incorporated into these cells by two different mechanisms, passive diffusion which is important at the beginning of the incubation, and active transport. Specificity assays suggested that the transporter involved in δ-aminolevulinic acid incorporation is a BETA transporter, probably GAT-2. © 2002 Cancer Research UK. Fil:Bermúdez Moretti, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Correa García, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Perotti, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Batlle, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Casas, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2002 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00070920_v87_n4_p471_BermudezMoretti http://hdl.handle.net/20.500.12110/paper_00070920_v87_n4_p471_BermudezMoretti |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
BETA transporters Membrane transport Photodynamic therapy δ-aminolevulinic acid aminolevulinic acid carrier protein animal cell animal model article breast adenocarcinoma cancer cell culture cell membrane transport controlled study diffusion drug transport drug uptake mouse nonhuman priority journal transport kinetics Adenocarcinoma Aminolevulinic Acid Animals Biological Transport, Active Mammary Neoplasms, Experimental Mice Tumor Cells, Cultured |
| spellingShingle |
BETA transporters Membrane transport Photodynamic therapy δ-aminolevulinic acid aminolevulinic acid carrier protein animal cell animal model article breast adenocarcinoma cancer cell culture cell membrane transport controlled study diffusion drug transport drug uptake mouse nonhuman priority journal transport kinetics Adenocarcinoma Aminolevulinic Acid Animals Biological Transport, Active Mammary Neoplasms, Experimental Mice Tumor Cells, Cultured Bermudez Moretti, Mariana Correa García, Susana Raquel Perotti, Christian Pablo Batlle, Alcira María del Carmen Casas, Adriana Gabriela δ-aminolevulinic acid transport in murine mammary adenocarcinoma cells is mediated by beta transporters |
| topic_facet |
BETA transporters Membrane transport Photodynamic therapy δ-aminolevulinic acid aminolevulinic acid carrier protein animal cell animal model article breast adenocarcinoma cancer cell culture cell membrane transport controlled study diffusion drug transport drug uptake mouse nonhuman priority journal transport kinetics Adenocarcinoma Aminolevulinic Acid Animals Biological Transport, Active Mammary Neoplasms, Experimental Mice Tumor Cells, Cultured |
| description |
δ-aminolevulinic acid, the precursor of porphyrin biosynthesis has been used to induce the endogenous synthesis of the photosensitiser protoporphyrin IX for photodynamic therapy in the treatment of various tumours. The aim of this work was to characterise the δ-aminolevulinic acid transport system in the murine mammary adenocarcinoma cell line LM3 using 14C-δ-aminolevulinic acid, to finally improve δ-aminolevulinic acid incorporation in mammalian cells. Our results showed that δ aminolevulinic acid is incorporated into these cells by two different mechanisms, passive diffusion which is important at the beginning of the incubation, and active transport. Specificity assays suggested that the transporter involved in δ-aminolevulinic acid incorporation is a BETA transporter, probably GAT-2. © 2002 Cancer Research UK. |
| author |
Bermudez Moretti, Mariana Correa García, Susana Raquel Perotti, Christian Pablo Batlle, Alcira María del Carmen Casas, Adriana Gabriela |
| author_facet |
Bermudez Moretti, Mariana Correa García, Susana Raquel Perotti, Christian Pablo Batlle, Alcira María del Carmen Casas, Adriana Gabriela |
| author_sort |
Bermudez Moretti, Mariana |
| title |
δ-aminolevulinic acid transport in murine mammary adenocarcinoma cells is mediated by beta transporters |
| title_short |
δ-aminolevulinic acid transport in murine mammary adenocarcinoma cells is mediated by beta transporters |
| title_full |
δ-aminolevulinic acid transport in murine mammary adenocarcinoma cells is mediated by beta transporters |
| title_fullStr |
δ-aminolevulinic acid transport in murine mammary adenocarcinoma cells is mediated by beta transporters |
| title_full_unstemmed |
δ-aminolevulinic acid transport in murine mammary adenocarcinoma cells is mediated by beta transporters |
| title_sort |
δ-aminolevulinic acid transport in murine mammary adenocarcinoma cells is mediated by beta transporters |
| publishDate |
2002 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00070920_v87_n4_p471_BermudezMoretti http://hdl.handle.net/20.500.12110/paper_00070920_v87_n4_p471_BermudezMoretti |
| work_keys_str_mv |
AT bermudezmorettimariana daminolevulinicacidtransportinmurinemammaryadenocarcinomacellsismediatedbybetatransporters AT correagarciasusanaraquel daminolevulinicacidtransportinmurinemammaryadenocarcinomacellsismediatedbybetatransporters AT perottichristianpablo daminolevulinicacidtransportinmurinemammaryadenocarcinomacellsismediatedbybetatransporters AT batllealciramariadelcarmen daminolevulinicacidtransportinmurinemammaryadenocarcinomacellsismediatedbybetatransporters AT casasadrianagabriela daminolevulinicacidtransportinmurinemammaryadenocarcinomacellsismediatedbybetatransporters |
| _version_ |
1768543732516257792 |