Progesterone treatment reduces NADPH-diaphorase/nitric oxide synthase in Wobbler mouse motoneuron disease
Previous work demonstrated that progesterone (PROG) treatment attenuates morphological, molecular and functional abnormalities in the spinal cord of the Wobbler (Wr) mouse, a genetic model of motoneuron degeneration. Wr mice show a marked up-regulation of the nitric oxide synthesizing enzyme (NOS)....
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2004
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00068993_v1014_n1-2_p71_GonzalezDeniselle http://hdl.handle.net/20.500.12110/paper_00068993_v1014_n1-2_p71_GonzalezDeniselle |
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paper:paper_00068993_v1014_n1-2_p71_GonzalezDeniselle2023-06-08T14:31:28Z Progesterone treatment reduces NADPH-diaphorase/nitric oxide synthase in Wobbler mouse motoneuron disease Endocrine and autonomic regulation Motoneuron disease Neuroendocrine regulation: other Neuroprotection Nitric oxide synthase Progesterone Wobbler mouse 3alpha hydroxy 5alpha pregnan 20 one 5alpha pregnane 3,20 dione methylprednisolone nitric oxide synthase progesterone reduced nicotinamide adenine dinucleotide phosphate dehydrogenase animal model animal tissue article astrocyte cell vacuole controlled study disease course drug blood level drug effect drug potency enzyme activity female histochemistry male motoneuron motor neuron disease mouse neuroprotection nonhuman phenotype priority journal white matter Animals Astrocytes Cell Count Down-Regulation Female Male Mice Mice, Neurologic Mutants Motor Neuron Disease Motor Neurons NADPH Dehydrogenase Nitric Oxide Synthase Progesterone Previous work demonstrated that progesterone (PROG) treatment attenuates morphological, molecular and functional abnormalities in the spinal cord of the Wobbler (Wr) mouse, a genetic model of motoneuron degeneration. Wr mice show a marked up-regulation of the nitric oxide synthesizing enzyme (NOS). Since nitric oxide is a highly reactive species, it may play a role in neuropathology of Wr mice. We now studied if PROG neuroprotection involved changes of NOS activity in motoneurons and astrocytes, determined by the nicotinamide adenine dinucleotide phosphate-diaphorase (NADPHD) histochemical reaction. Two and four-month-old Wr mice at the progressive and stabilization stages of the disease, respectively, and their age-matched controls were left untreated or received a single 20-mg PROG pellet for 18 days. PROG reduced the high number of NADPHD-active motoneurons and white matter astrocytes in 2-month-old Wr mice but was unable to change the low number of NADPHD-active motoneurons in 4-month-old Wr mice or astrocytes in this age group. A large number of motoneurons in 2-month-old Wr mice showed a vacuolated phenotype, which was significantly reverted by PROG treatment. In summary, PROG treatment during the early symptomatic stage of the disease caused a significant reduction of NADPHD-active motoneurons and astrocytes and also reduced vacuolated degenerating cells, suggesting that blockade of NO synthesis and oxidative damage may contribute to steroid neuroprotection. © 2004 Elsevier B.V. All rights reserved. 2004 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00068993_v1014_n1-2_p71_GonzalezDeniselle http://hdl.handle.net/20.500.12110/paper_00068993_v1014_n1-2_p71_GonzalezDeniselle |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Endocrine and autonomic regulation Motoneuron disease Neuroendocrine regulation: other Neuroprotection Nitric oxide synthase Progesterone Wobbler mouse 3alpha hydroxy 5alpha pregnan 20 one 5alpha pregnane 3,20 dione methylprednisolone nitric oxide synthase progesterone reduced nicotinamide adenine dinucleotide phosphate dehydrogenase animal model animal tissue article astrocyte cell vacuole controlled study disease course drug blood level drug effect drug potency enzyme activity female histochemistry male motoneuron motor neuron disease mouse neuroprotection nonhuman phenotype priority journal white matter Animals Astrocytes Cell Count Down-Regulation Female Male Mice Mice, Neurologic Mutants Motor Neuron Disease Motor Neurons NADPH Dehydrogenase Nitric Oxide Synthase Progesterone |
| spellingShingle |
Endocrine and autonomic regulation Motoneuron disease Neuroendocrine regulation: other Neuroprotection Nitric oxide synthase Progesterone Wobbler mouse 3alpha hydroxy 5alpha pregnan 20 one 5alpha pregnane 3,20 dione methylprednisolone nitric oxide synthase progesterone reduced nicotinamide adenine dinucleotide phosphate dehydrogenase animal model animal tissue article astrocyte cell vacuole controlled study disease course drug blood level drug effect drug potency enzyme activity female histochemistry male motoneuron motor neuron disease mouse neuroprotection nonhuman phenotype priority journal white matter Animals Astrocytes Cell Count Down-Regulation Female Male Mice Mice, Neurologic Mutants Motor Neuron Disease Motor Neurons NADPH Dehydrogenase Nitric Oxide Synthase Progesterone Progesterone treatment reduces NADPH-diaphorase/nitric oxide synthase in Wobbler mouse motoneuron disease |
| topic_facet |
Endocrine and autonomic regulation Motoneuron disease Neuroendocrine regulation: other Neuroprotection Nitric oxide synthase Progesterone Wobbler mouse 3alpha hydroxy 5alpha pregnan 20 one 5alpha pregnane 3,20 dione methylprednisolone nitric oxide synthase progesterone reduced nicotinamide adenine dinucleotide phosphate dehydrogenase animal model animal tissue article astrocyte cell vacuole controlled study disease course drug blood level drug effect drug potency enzyme activity female histochemistry male motoneuron motor neuron disease mouse neuroprotection nonhuman phenotype priority journal white matter Animals Astrocytes Cell Count Down-Regulation Female Male Mice Mice, Neurologic Mutants Motor Neuron Disease Motor Neurons NADPH Dehydrogenase Nitric Oxide Synthase Progesterone |
| description |
Previous work demonstrated that progesterone (PROG) treatment attenuates morphological, molecular and functional abnormalities in the spinal cord of the Wobbler (Wr) mouse, a genetic model of motoneuron degeneration. Wr mice show a marked up-regulation of the nitric oxide synthesizing enzyme (NOS). Since nitric oxide is a highly reactive species, it may play a role in neuropathology of Wr mice. We now studied if PROG neuroprotection involved changes of NOS activity in motoneurons and astrocytes, determined by the nicotinamide adenine dinucleotide phosphate-diaphorase (NADPHD) histochemical reaction. Two and four-month-old Wr mice at the progressive and stabilization stages of the disease, respectively, and their age-matched controls were left untreated or received a single 20-mg PROG pellet for 18 days. PROG reduced the high number of NADPHD-active motoneurons and white matter astrocytes in 2-month-old Wr mice but was unable to change the low number of NADPHD-active motoneurons in 4-month-old Wr mice or astrocytes in this age group. A large number of motoneurons in 2-month-old Wr mice showed a vacuolated phenotype, which was significantly reverted by PROG treatment. In summary, PROG treatment during the early symptomatic stage of the disease caused a significant reduction of NADPHD-active motoneurons and astrocytes and also reduced vacuolated degenerating cells, suggesting that blockade of NO synthesis and oxidative damage may contribute to steroid neuroprotection. © 2004 Elsevier B.V. All rights reserved. |
| title |
Progesterone treatment reduces NADPH-diaphorase/nitric oxide synthase in Wobbler mouse motoneuron disease |
| title_short |
Progesterone treatment reduces NADPH-diaphorase/nitric oxide synthase in Wobbler mouse motoneuron disease |
| title_full |
Progesterone treatment reduces NADPH-diaphorase/nitric oxide synthase in Wobbler mouse motoneuron disease |
| title_fullStr |
Progesterone treatment reduces NADPH-diaphorase/nitric oxide synthase in Wobbler mouse motoneuron disease |
| title_full_unstemmed |
Progesterone treatment reduces NADPH-diaphorase/nitric oxide synthase in Wobbler mouse motoneuron disease |
| title_sort |
progesterone treatment reduces nadph-diaphorase/nitric oxide synthase in wobbler mouse motoneuron disease |
| publishDate |
2004 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00068993_v1014_n1-2_p71_GonzalezDeniselle http://hdl.handle.net/20.500.12110/paper_00068993_v1014_n1-2_p71_GonzalezDeniselle |
| _version_ |
1768544531647561728 |