Anomalous dynamics of melanosomes driven by myosin-V in Xenopus laevis melanophores
The organization of the cytoplasm is regulated by molecular motors, which transport organelles and other cargoes along cytoskeleton tracks. In this work, we use single particle tracking to study the in vivo regulation of the transport driven by myosin-V along actin filaments in Xenopus laevis melano...
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2009
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00063495_v97_n6_p1548_Brunstein http://hdl.handle.net/20.500.12110/paper_00063495_v97_n6_p1548_Brunstein |
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paper:paper_00063495_v97_n6_p1548_Brunstein2023-06-08T14:31:19Z Anomalous dynamics of melanosomes driven by myosin-V in Xenopus laevis melanophores Bruno, Luciana Despósito, Marcelo Arnaldo Levi, Valeria actin molecular motor myosin V actin filament active transport animal cell article cell organelle controlled study diffusion melanophore melanosome microtubule assembly nonhuman prediction transport diffusion model Xenopus laevis Xenopus laevis The organization of the cytoplasm is regulated by molecular motors, which transport organelles and other cargoes along cytoskeleton tracks. In this work, we use single particle tracking to study the in vivo regulation of the transport driven by myosin-V along actin filaments in Xenopus laevis melanophores. Melanophores have pigment organelles or melanosomes, which, in response to hormones, disperse in the cytoplasm or aggregate in the perinuclear region. We followed the motion of melanosomes in cells treated to depolymerize microtubules during aggregation and dispersion, focusing the analysis on the dynamics of these organelles in a time window not explored before to our knowledge. These data could not be explained by previous models that only consider active transport. We proposed a transport-diffusion model in which melanosomes may detach from actin tracks and reattach to nearby filaments to resume the active motion after a given time of diffusion. This model predicts that organelles spend -70% and 10% of the total time in active transport during dispersion and aggregation, respectively. Our results suggest that the transport along actin filaments and the switching from actin to microtubule networks are regulated by changes in the diffusion time between periods of active motion driven by myosin-V. © 2009 by the Biophysical Society. Fil:Bruno, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Desposito, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Levi, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2009 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00063495_v97_n6_p1548_Brunstein http://hdl.handle.net/20.500.12110/paper_00063495_v97_n6_p1548_Brunstein |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
actin molecular motor myosin V actin filament active transport animal cell article cell organelle controlled study diffusion melanophore melanosome microtubule assembly nonhuman prediction transport diffusion model Xenopus laevis Xenopus laevis |
| spellingShingle |
actin molecular motor myosin V actin filament active transport animal cell article cell organelle controlled study diffusion melanophore melanosome microtubule assembly nonhuman prediction transport diffusion model Xenopus laevis Xenopus laevis Bruno, Luciana Despósito, Marcelo Arnaldo Levi, Valeria Anomalous dynamics of melanosomes driven by myosin-V in Xenopus laevis melanophores |
| topic_facet |
actin molecular motor myosin V actin filament active transport animal cell article cell organelle controlled study diffusion melanophore melanosome microtubule assembly nonhuman prediction transport diffusion model Xenopus laevis Xenopus laevis |
| description |
The organization of the cytoplasm is regulated by molecular motors, which transport organelles and other cargoes along cytoskeleton tracks. In this work, we use single particle tracking to study the in vivo regulation of the transport driven by myosin-V along actin filaments in Xenopus laevis melanophores. Melanophores have pigment organelles or melanosomes, which, in response to hormones, disperse in the cytoplasm or aggregate in the perinuclear region. We followed the motion of melanosomes in cells treated to depolymerize microtubules during aggregation and dispersion, focusing the analysis on the dynamics of these organelles in a time window not explored before to our knowledge. These data could not be explained by previous models that only consider active transport. We proposed a transport-diffusion model in which melanosomes may detach from actin tracks and reattach to nearby filaments to resume the active motion after a given time of diffusion. This model predicts that organelles spend -70% and 10% of the total time in active transport during dispersion and aggregation, respectively. Our results suggest that the transport along actin filaments and the switching from actin to microtubule networks are regulated by changes in the diffusion time between periods of active motion driven by myosin-V. © 2009 by the Biophysical Society. |
| author |
Bruno, Luciana Despósito, Marcelo Arnaldo Levi, Valeria |
| author_facet |
Bruno, Luciana Despósito, Marcelo Arnaldo Levi, Valeria |
| author_sort |
Bruno, Luciana |
| title |
Anomalous dynamics of melanosomes driven by myosin-V in Xenopus laevis melanophores |
| title_short |
Anomalous dynamics of melanosomes driven by myosin-V in Xenopus laevis melanophores |
| title_full |
Anomalous dynamics of melanosomes driven by myosin-V in Xenopus laevis melanophores |
| title_fullStr |
Anomalous dynamics of melanosomes driven by myosin-V in Xenopus laevis melanophores |
| title_full_unstemmed |
Anomalous dynamics of melanosomes driven by myosin-V in Xenopus laevis melanophores |
| title_sort |
anomalous dynamics of melanosomes driven by myosin-v in xenopus laevis melanophores |
| publishDate |
2009 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00063495_v97_n6_p1548_Brunstein http://hdl.handle.net/20.500.12110/paper_00063495_v97_n6_p1548_Brunstein |
| work_keys_str_mv |
AT brunoluciana anomalousdynamicsofmelanosomesdrivenbymyosinvinxenopuslaevismelanophores AT despositomarceloarnaldo anomalousdynamicsofmelanosomesdrivenbymyosinvinxenopuslaevismelanophores AT levivaleria anomalousdynamicsofmelanosomesdrivenbymyosinvinxenopuslaevismelanophores |
| _version_ |
1768545719695704064 |